FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813611
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9765312.002418846352864No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA204812.5172963492381495No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG121451.4927280973339838No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG70920.871669630818659No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA66290.8147628289194714No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.6877979771659921No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49230.6050803147941707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.5664869329446136No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC44330.5448549736913586No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC36400.4473882481923179No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA32810.4032639676700536No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG29930.36786621616472737No Hit
CTCCAGCTTGTGGTTGATTTGAGACACAGACTGGGCTTTGTGGCAAAGCT28600.35151933786539263No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA26670.32779792800244834No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA26630.32730629256487437No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC22750.2796176551201987No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC22560.27728238679172235No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22070.2712598526814411No Hit
CTCTCGGTGAGCGCACGTCCCGTGCTCCCCTCTGGCGGGTGCGCGCGGGC19030.23389555942581897No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.2255377569870614Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18180.223448306377372No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC18110.2225879443616175No Hit
GCACGTGGCAGCGCGCCTGGCCCTGGGCTTGGAGGCGCCGGCGCCCTGGA17460.21459886850104046No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATG17130.21054287614105513No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT17030.20931378754712018No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA16880.2074701546562178No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC16650.2046432508901674No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15580.19149200293506358No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT14970.18399456251206042No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA14790.18178220304297754No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT14390.17686584866723778No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC14250.17514512463572887No Hit
GCATGTTACCTTCAACCAAGTGAAAGGCATTTTCGGCTTCACTGACAGCG14210.17465348919815488No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG14210.17465348919815488No Hit
ACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC13680.1681393196502997No Hit
GGAGAGGAGGCCCCGCAGATGAACTACATCCAGGTGACGCCGCAGGAGAA13320.16371460071213392No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13110.16113351466487055No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC13040.1602731526491161No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12840.15781497546124623No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12690.1559713425703438No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12640.15535679827337634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12600.15486516283580237No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC12430.15277571222611297No Hit
GCCTGGAATGCTCTTGTCCAGGTGGAGATATGCCACATGGCTCTCTCACC12280.15093207933521055No Hit
GAATCTGCTCTCGGAATGGATGACTCTTCTCTGACAGTAGACAATGCAAA12210.1500717173194561No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC11970.14712190469401226No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11840.14552408952189683No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11810.14515536294371634No Hit
GTTGTACCACTGTCAGAGCCAGGCTCCTCTTTGCCTTCCATCCCTGGCAC11780.14478663636553585No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11350.13950155541161563No Hit
GTGCATCCAGCGCACGCACTTGAAGACTTCTGCAGCACTGGGACCCCGCT10920.13421647445769538No Hit
TTCCGAACCCGCTGCTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTT10910.13409356559830188No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA10890.1338477478795149No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10820.13298738586376044No Hit
GCCCAGGGGCAGCGCATAGTGGGACTCGTACCACTGTCGGTACGGTGTGC10270.12622739859711826No Hit
CTCTCAGCCCTCCACACACACTCACCCCCACTCCCACACACATACACACA10090.12401503912803538No Hit
GGTTTAGGAGTGGGCAGAGTTTATCACCAGCTGGTCACAGTTGAAAAGTG9940.12217140623713298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9890.12155686194016552No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG9760.11995904676805008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.11971322904926311No Hit
GTGACTGTCGTCTCCTGGACTTCACGTGGACGTTCTTCTCCGTAGGGCCC9710.11934450247108262No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC9660.11872995817411515No Hit
GGACTTGCTCACGCTTCCTGGTACCACAGAGCACTTGCGGTTTACCCGGC9580.11774668729896719No Hit
TCCATGAGTGCACATTTCTCCTCCACCTCTAGGTCAATGCACCAAATGAC9450.11614887212685178No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT9360.11504269239231034No Hit
GAGAAGAAACTTTGGATTTGCTGTTTCCAGCAAAAGCCTTGAAGTCTGAC9200.11307615064201441No Hit
CTCCATGAGTGCACATTTCTCCTCCACCTCTAGGTCAATGCACCAAATGA9160.11258451520444045No Hit
GTGTCATATTTCCTCTTGCTGGAGTACCACAATAGCAGGGGGATGCCGAT9080.1116012443292925No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC9020.11086379117293153No Hit
GTCCAGCCCCGTGCAGGCCACGTTTGAGGTTCTTGATTTCATCACGCACC8890.10926597600081611No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC8760.1076681608287007No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8740.1074223431099137No Hit
CAGAAAAGGCATCAGGGCTCACAGTCCGAAGAGTTTGGTTACGGAGTCTC8620.10594743679719178No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8440.1037350773281089No Hit
GTACATGGGGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACC8330.10238307987478046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8310.10213726215599346No Hit
GCAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA8240.101276900140239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)