FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648879
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT486597.498932774831671No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA130672.0137806894659867No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59550.9177365887939045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43950.6773219660368112No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA42820.6599073170806884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.5555735352816165No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC32890.5068741629795386No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG32730.5044083719769017No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC30330.46742150693734885No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG24900.3837387247853606No Hit
GAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGG21540.3319571137299866No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT20870.32163161390644485No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19490.30036416650870196No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC18730.28865165924617686No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA18460.28449063692922716No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG17970.2769391519836518No Hit
GACCAGACTGGTTTTGGAGGTGGCCCAGCATTTGGGTGAGAGCACAGTAA16370.25228124195728324No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT16260.25058601064297037No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT15850.24426742119871345No Hit
CTCCGCCTCCCCCCGCCCGCCAGCCGAGGCAGCTCGAGCCCAGTCCGCGG14750.22731510805558508No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14400.22192119023731696No Hit
ATCTGCACACACCTCCGAGACAGTCCAGTGTCACCTCTCTCAGAGCATCT14110.21745194404503768No Hit
ATGTCATAGATGGCCTCATTGTCTACCATGAAGGCACAATCAGAGTGCTC13530.20851345166047908No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13290.20481476515652378No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC13180.20311953384221093No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.20219486221622215Clontech SMART CDS Primer II A (100% over 21bp)
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC12770.19680094439795398No Hit
GGTCATGAAAGCGTGACGGCCACCACATCACAAGCGTGATGGCACCTGCT12350.19032824301603227No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11310.1743006014988927No Hit
GATCGCTCCTGCTGCTCCTCTCCTCTCGGGGCGGCGGCGGCGGGGGCGCC11230.1730677059975743No Hit
CTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGT11210.17275948212224468No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11130.17152658662092624No Hit
CGAGTAAGTGCTTCAGTTTCTCCACCTGGCCACACTGCAGAGGATAAGCC10970.1690607956182894No Hit
GTACATGGGGATTGCGAGCCGAACCCGGCAGCTGGCGCCATGGTGCTGTT10890.16782790011697096No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10450.1610469748597196No Hit
ATGTAAGTCATGTTAGTGGGGTCCAGCTCCTTGGCTTTGTCGTAATGCTT10380.159968191296066No Hit
AGATTACACTGGTGGGCCACAGTACTGCCACCACACAGAAGTTCAGGTTA10130.1561153928544459No Hit
GCATCTGATGGCCTTTGTTCTCTTCTCCGAGCCTTGAGTCAGGGATGGGA10110.15580716897911628No Hit
TGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGA10070.15519072122845706No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10050.15488249735312748No Hit
GAAGCTGACGGCGGTGTGCATGGAGTGCTTCCGGGAAGCCGCCTATACCA9870.152108482475161No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9830.1514920347245018No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9770.15056736309851299No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA9580.1476392362828817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.1467145646568929No Hit
CGTCTCACATCGGAGTTGGCTCAGACCCTGGCTGTGCATCCATCAGAAAG9480.14609811690623367No Hit
AGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAAC9370.14440288559192083No Hit
TCTATATCTTGAGCCTTAGACAGCTCCACCAGCATTCTGGCTGCTGGATG9370.14440288559192083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9300.1433241020282672No Hit
CCACCAAGGTGGTGACGGTGTTAACTCCTGCTCGAAGGACAGGTGGTCTC9150.14101242296329516No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9030.13916307971131753No Hit
AGTCTGTGAAGCTTTAATGCAAATTGTTGTCCTTCTTCATTTGCAACAAT8810.13577261708269184No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA8780.13531028126969744No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8720.1343856096437086No Hit
TTCCTTACCACATTGTGAATATTGTCTCAGGTTCTTTGAATCATGCTGCC8620.13284449026706058No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8480.13068692313975333No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8450.13022458732675893No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8410.1296081395760997No Hit
ACTTCCAGCTCCTTGACGTTGTGGACCAGGAACTTCCGGAAGCCACTGGG8360.1288375798877757No Hit
CTGGAGCAGTGTGTCTGGGAAAGCCTCAGACATATAATGCCACCCTCTGA8360.1288375798877757No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTG8300.12791290826178686No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC8230.12683412469813324No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC8160.12575534113447961No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8140.12544711725915003No Hit
CTTTTCGTCGTCTCCATGGGAACCAATTCAGGACAATCCTCCTCCGCAGG8020.12359777400717237No Hit
GAATAATAAAGACGGTGCGTGCAATGGCATCTGCCTTGGTACAGGTCAAC7740.11928263975255787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7720.11897441587722826No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT7710.11882030393956346No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC7640.11774152037590983No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC7580.11681684874992102No Hit
CACAGGCCTCGTGCCTCCGTCGGAGCGCAGAGGCGGCACTTACTCTGCAG7580.11681684874992102No Hit
GCCATTCGTCGTAAACATTGCGTCCACTTGGCATCGGAGGAGGTGCAACA6950.10710779667703839No Hit
TCCACGTGGACAGTTGTGCCGTTGGCCTTCTCACGCTGCACCCGCTCGAT6910.10649134892637918No Hit
GTACATGGGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG6800.10479611761206635No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6790.10464200567440154No Hit
TTCTAGATGGTCTGCTTCTTTCACAGTAACTAAAGGCAAAAGTAGTACAC6660.10263855048475909No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG6630.1021762146717647No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6570.10125154304577587No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG6540.10078920723278145No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC6520.10048098335745186No Hit
GGATTGTTCCTATGTTTTTTCAACGTGTCTTCTGCTTTTCCACTCGAGGA6500.10017275948212225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)