FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696015
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT364335.234513623987989No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA101811.4627558314116793No Hit
CCTGCGAAGATGCTTCCCTGTGCAAGAGGTGCCTGTCGGGCGCGGGGTTC49150.7061629418906202No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG48530.6972550878932207No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.5790105098309662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.4793000150858818No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA33110.47570813847402715No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC29320.421255289038311No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26100.37499191827762335No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC24540.3525786082196504No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24370.3501361321235893No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT20890.30013720968657287No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20000.28735012894837036No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA19800.28447662765888665No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG16430.23605813093108627No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC15340.22039754890340008No Hit
AGTAGAACTCCACCCGCATTATGCGCACGTTGTACATGCGTGTCAGTCGC15330.2202538738389259No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15110.21709302242049383No Hit
GTACATGGGGGGCGGCGGCCTGGGCGAGCTCGAATCCGGCCTTGGGCAAG14940.21465054632443265No Hit
GCTAAAGAGAGAGTTCGTCAGGATCCTTGTGAGGATATTTCTCGTATCCA14930.2145068712599585No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13930.20013936481253994No Hit
GTAGACAGCAGCCAGCACCTTCTCGGTCACATACTGGCAGCGCTTCAAGT13710.19697851339410788No Hit
GCAATGGAGTGACACAGCACAAAGCCCTCTAGACTGTCACTACCATCTGC13510.19410501210462416No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT13180.1893637349769761No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13120.18850168459013095No Hit
TCTGCGAAGATGCTTCCCTGTGCAAGAGGTGCCTGTCGGGCGCGGGGTTC13060.18763963420328583No Hit
GTAGGCATCCAACCAGTGGACTCGGTTAGATTTGCTTTTGATGAAAGAAA12700.18246733188221517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.18246733188221517Clontech SMART CDS Primer II A (100% over 21bp)
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT12540.18016853085062823No Hit
AACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCA12520.17988118072167983No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12500.17959383059273146No Hit
GGTTTCGGCCAGCCAGGCTGGACCCTGGAGCCGAGGTTGGGGTCTCCTCA12080.1735594778848157No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG12020.1726974274979706No Hit
GTATTAAGAGGGGGGCAGCACATTGGAAGCCCTCATGAGTGCAGGGCCCG11910.17111700178875455No Hit
CTGCGGTGCTTTCCTACCAGGGTAGAGTTCCAAACTCCAAGACTGAAGTA11860.17039862646638362No Hit
CTTAAACACCGTGTATCCCCGCTCCAGGTCGCTCTCTTCATAGCGAAAGG11700.16809982543479668No Hit
GACTAAGTGGAGATGTTGAATGGATAGTTGAATAACAGAGTCAGATGTTC11670.1676688002413741No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10740.1543070192452749No Hit
GCTATGACCCTTGCGAAGCAGTGCTGGTGGAAAGCATTCCTGAGGGCCTG10700.15373231898737813No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC10660.1531576187294814No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10360.14884736679525584No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT10350.14870369173078166No Hit
GGATTGATGGGAAGGGACAGATGTAGTTGGCAACCATGGTTAGCATCGGA10280.14769796627946236No Hit
GATTCTGTGCTTGTGACATTCCCTTGTACCAGGCCCTCCACAAAGAGCTG10230.14697959095709143No Hit
CTTGACTCTTTTTTCTTCTGAGAGGCCCAACCCACCGTGGAAAGTTTATC10190.14640489069919468No Hit
GGTAAAGGGGAACCAGTGCTGGCACCAATGGAAGTCCAAAGAGGGGATCC10100.145111815118927No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC10010.14381873953865937No Hit
ACTCTGGAGTTCCAAAGATGTCAGAGATATGGATGACGCCTATGACAGAC9720.13965216266890798No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA9610.13807173695969197No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9600.13792806189521778No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9410.13519823567020828No Hit
GTCTAAGCTATGGAAAAGCTCCCCTTATCCAACAGAGTTTAAAAGTAGTG9410.13519823567020828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.13289943463862128No Hit
ATCTAGTTCAAGTCACACCTACTTCACTCTTAGACTTCTTCCTTCAAGTG9140.13131900892940526No Hit
TGCTGGTGTACTTCTACACAAGGCCTCTTAGTGACGTCAATCAATTGGAG9100.13074430867150852No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8950.12858918270439573No Hit
AATCCCTTCCATGTCGAAGAACCGAGTCCCCGACTCCTGCTGCATTAATG8940.12844550763992157No Hit
GAACACAGATGATGAAGCCTGATGCCGGGCAATGTCGCGGTGATTCATGG8910.128014482446499No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT8510.1222674798675316No Hit
GTGACAGTTACGCAAATCAGCTTGAATTCCATATGTCCCTGAGTTATTTT8360.12011235390041881No Hit
CTCTCTGTCTGTGTCCTGGACGCAGCACTGAGCACCTGGGGAAGATGGCT8280.11896295338462533No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8240.11838825312672858No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8200.11781355286883184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8180.11752620273988348No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8130.11680782741751254No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG8090.11623312715961581No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8060.11580210196619327No Hit
GCCTATGGGTACTAGTTTTATTGTTTTTGTAACCCCTCAACTCATTTGCA7980.11465270145039977No Hit
CTCACTTTCTGTCCAGTTTTGTTCCCTTGCTGGCGAGGAGTTGTGATCCT7950.11422167625697723No Hit
GGCTCTGGGTGAAGAGCGGCGCAGCGGCGGCGGCGGCGGGCGGGGGGCGC7830.11249757548328698No Hit
CTAAACAACTTCTTGCCATTCCCAAATACACCACTGTCTCCTCCTGCATC7730.11106082483854514No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG7710.11077347470959678No Hit
GAGCTGCAGAAATCCTAGCTTGGGGGTTTCTCTCTCTCCAGGCTTTCTGC7480.10746894822669052No Hit
AAACTGTAGTTTGGCTTCATACCTTTAATTCCTCTTTCAGCATGACTACT7460.10718159809774215No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7460.10718159809774215No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7410.10646322277537122No Hit
GTCCCATCTTCAGAAAGTTAAAAGGGTCCATTTGGGATCCCAGAGCCTAG7390.10617587264642285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7210.1035897214858875No Hit
TAACTGGGGCGTACACAGTGACCCTGGATGGACGAGGGCCCTCAAGGGTC7190.10330237135693913No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA7140.10258399603456823No Hit
GAGTTGGGGCTGACATCTAGCCCAAGCTCCAATCGCCAGGCCATGCACAC7050.10129092045430056No Hit
GTCTGAGACTCCCCCGGACCGCTGGGTGCTGCTTGGAGCCCCTCCTGGCC6970.10014151993850708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)