FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658611
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA167292.5400426048152855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53540.8129229545209539No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48030.7292620378341692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47120.7154450806318146No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA40670.6175117026590811No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC36460.5535892962613743No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25170.3821679261354578No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT23260.3531674994799662No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG21110.32052304015572164No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA21000.3188528585158766No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20620.31308314012368454No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC19830.30108819925570635No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT18950.2877267461369458No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC17480.2654070460408344No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA17290.2625221868447384No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.2569043031470777Clontech SMART CDS Primer II A (100% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15870.2409616602212839No Hit
GTAAAGTGCCAACCATATCCATCAACAAAACAGATGGCTGCCATGCTTAC13750.2087727049806335No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13420.20376216006109826No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC12610.19146354980405733No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12580.19100804572046323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11970.18174612935404966No Hit
GATGAGCTCATCTGCCAGGCACTCAGCAATGGTCTTAATGTTCCGGAAGG11810.17931677424154774No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA11780.17886127015795364No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11670.17719108851810855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.17719108851810855No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC11230.1705103619587283No Hit
GTCAAACACACTCCTGGGGATCAGGACTGTGATGAGCTTGGCCGCGAGAC11160.1694475190970087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10800.1639814700938794No Hit
AGTCACATGTCTCGGTATCTTGCTCACAAAGTGCACCAACATAACTTGGA10600.16094477620325198No Hit
ATCTACTACCACATAAAGGTGGCGCATCATTCCAAGTCGAACTTGTCCAT10450.15866725578528143No Hit
ACTCTGAAGTTAGATCCTATCACAGGGCGATCAAGGGGTTTTGGCTTTGT10350.15714890883996774No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10170.1544158843384031No Hit
GTGAATGCTTGCATGCATTCACACGTGTGTGTGTCCCAGCTAGTTCACTC9810.14894983533527378No Hit
GTGCAGGGTAGACAACAGGTCCTTGTTGAAGATGCTCTCCACGCTCCACT9760.14819066186261692No Hit
GCAATCGCAGGCACTCAGCAATGGTCTTAATGTTCCGGAAGGCAGCCTCA9730.14773515777902282No Hit
GTATAAAGCGCCGCTCAGTTGTCTCCTGGAAGACGGGCGTCTTCCGAAAC9510.14439479449933268No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC9480.14393929041573858No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC9320.14150993530323666No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG9140.138776910801672No Hit
CTAGGTGATCTGTGAAAATGGTTCGCTATTCACTTGACCCGGAGAACCCC9000.13665122507823282No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8910.1352847128274505No Hit
ACCAAGGACTTAAAGGAGAAGAAGGAAGTTGTGGAAGAGGCAGAAAATGG8790.13346269649307405No Hit
TAAGAGAACTTTCCCCGTGTCTCACAGGTAGGTAGAGGCAGAGCTGGAAC8740.1327035230204172No Hit
GTGCAGTTCGTCTGTGACAGACAGGAGGCAGCTGCTTCTCTTGTGACCTC8700.13209618424229172No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT8670.13164068015869762No Hit
AAACAAGAGTTATTTGGTTCCAGAGGTTCAACGCCAGTTATTGTCAGTTC8650.1313370107696349No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8590.13042600260244666No Hit
AGACAGGGTTTCACCATGTTGACCAGGCTCATCTTGAACTCCTGACCTCA8490.12890765565713297No Hit
CTCCAGCCCGTGCAGTCCGCTCTTCACTGTTCCACGGCCTCCCAGTGCCT8290.12587096176650556No Hit
GTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCACCCACCTC8240.12511178829384872No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG8220.12480811890478599No Hit
GATGACACAGTGATAGAGCTCATTCCCTTCCCGGGTGACCAGCTGGTTCT8200.12450444951572323No Hit
CTCTAAGATCTACACTGGTGGCTGTGGCTGGGTCAATCACTTTCATCACT8050.1222269290977527No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG8040.12207509440322134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7850.1191902352071253No Hit
GTATTTGCCACTAAATTCCCCAAGTATGCCAACATTACAAAAAAGATAGG7840.11903840051259393No Hit
CAACGCAGAGTACATGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTG7790.11827922703993708No Hit
GTCTTCTACACATCCACAAGTAATTGTGCCAGGTCTACCATTGAATAGAA7780.11812739234540572No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC7770.11797555765087434No Hit
ATACAGTCCAGAAGTCCTGATGAGAGTTCTTCCGTAAATCCAGCACTTGA7680.11660904540009201No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7560.11478702906571557No Hit
GAGGTGAAGATGTCTTCTACCAACTCCATGTCATTGAGCATCAATGCCAA7460.11326868212040188No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT7370.11190216986961955No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC7370.11190216986961955No Hit
CCTCTAAGAAGTTGGGGGACGCAGACCGCTCGGGGGTCGCGTAACTAGTT7350.11159850048055682No Hit
GCATTTAGTGGTCCTGAACAGCAAGTGGAAAGACGCAGCAATTTGCCAGG7210.10947281475711763No Hit
GAGGAAAAGCAGGTGCCGGTGCCTGTCCCCGGGGGCGCCATGGCGACCGG7150.10856180658992941No Hit
GTACATGGGAGTGCTGCCAGTCAACCAAGGGTCTGTGAGTGTCAGCGTGG6900.10476593922664516No Hit
TCTTTCGGTGATCTCAGGAACCAGAGTCAGAGTTGTACTTTTTACCCTTA6740.10233658411414326No Hit
GTCAAGGAAAGTACACTCCGAGCGGTCAGGCTGGGGCTGCTGCCAGCGAG6730.10218474941961186No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA6660.1011219065578923No Hit
GGAAAGAACGTGCCTTAACGGCAGCCCCACCCAGAGCCTGCTGCGTGGCT6640.10081823716882954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)