FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531785
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT310895.846159632182179No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA91581.722124542813355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.7717404590200927No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38520.7243528869749993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.6611694575815414No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC32390.6090807375161015No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA30650.5763607472944893No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25710.4834660624124411No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22590.4247957351185159No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA20600.3873745968765573No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC20100.3779723008358641No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18580.34938932087215696No Hit
GCAATCTCCTTGCAGGATACAAATCATTTCATAATCTTACTCCTGCTTAA18230.3428077136436718No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16780.31554105512566166No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT16410.30858335605554876No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15450.29053094765741794No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15310.28789830476602385No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15100.2839493404289328No Hit
CAATAGCATAGAAGGAGGATATAACTGAATCCATAGCTATTGTCATCCCA13030.24502383482046317No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.24107487048337203Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTGGAGGACGGGTCGGTCCTGCGGGGCCAACCCTTTGGGGCCGCCGT12410.23336498773000366No Hit
CATCCGTGATGTGGCCAAGCAAGTGAAGGCCAGGTTGCGGGAGGCAGGCC11940.2245268294517521No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11760.2211420028771026No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11520.2166289007775699No Hit
CTTCCCAGAGAGCATCACAAGAAGGCGTCGCACCACTCTCGAAGGCATCC11180.21023533946989856No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT10800.2030895944789718No Hit
GAGTGCAGTGGCGTGATCTCGGCTCTCGGCTCACTACAACCTCTGCCTCC10620.19970476790432223No Hit
CTCCAGGGCCTTGACAAGCAGCTCATTCAAGCGGCCCACCATGGCCCTAG10420.195943849488045No Hit
GCAATCGCAGGCCACTCTGCTGAGCATCTTCTCCCAGGAGTACCAGAAAC10000.18804592081386276No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9850.1852252320016548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9830.18484914016002707No Hit
GAATAGATGTCTTCATCACTTCTTCCACTGTGTGTTGACACTGTTTTCCT9390.17657511964421713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.1756348900401478No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG9260.1741305226736369No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9150.17206201754468442No Hit
CTTCTGACCCCTGTGGCGTGCGTCCTGGCGCGCCGCACCCGCCTGGTGCC9070.1705576501781735No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9030.16980546649491807No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA9010.16942937465329033No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8650.16265972150399127No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC8570.16115535413748036No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC8560.1609673082166665No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8550.16077926229585263No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT8420.15833466532527243No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8230.15476179282980904No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG8120.15269328770085655No Hit
TCCCCGGAGTCACCCTTGCAGCTGTCCCGGCGATTGCTCTCCGCGCACAT8030.15100087441353177No Hit
CTGTAAGCTTTCCCCATTGTTCGTAGTAGTTCCTCAAACTTTCTTCTGTG7820.14705191007644067No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC7630.14347903758097727No Hit
CCCCCGGAGTCACCCTTGCAGCTGTCCCGGCGATTGCTCTCCGCGCACAT7360.138401797719003No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG7310.13746156811493365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6990.13144409864889006No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC6980.1312560527280762No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6980.1312560527280762No Hit
GGAAGGACAAGGACATATTCAGAGCACACAGAGGGAAGGACGAGGGACTT6960.13087996088644846No Hit
GAGAAACTCTTGGGACCTGGTGTGAATTATTCAGGGTGTCAGATCACATG6960.13087996088644846No Hit
GTTTCATCCTCGCCTTAGCATGATTTATCCTACACTCCAACTCATGAGAC6810.12805927207424053No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6750.12693099654935736No Hit
GAGCACCAGGTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCAC6670.12542662918284647No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6610.12429835365796327No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG6570.12354616997470783No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC6490.12204180260819693No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC6420.12072548116249988No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC6410.12053743524168602No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC6350.11940915971680284No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6170.11602433314215331No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6100.11470801169645628No Hit
GGAAACTTGGTCTAGCACAGGCAACTCAGCAGCATGAAAATCAACTGGAG6080.11433191985482855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5900.11094709328017902No Hit
CAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCT5740.10793835854715722No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA5620.10568180749739085No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT5590.10511766973494928No Hit
CTCCAAAGACGGCAGACTCACAGGAGACCAAGGAATCCCAGAAAGTGGAG5590.10511766973494928No Hit
GATGATAACTGCGCGGCATTCTTTGAGGGACCCCCATTCAAGTTCAAGTT5590.10511766973494928No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC5580.10492962381413543No Hit
GATTTGTCTTGCAAAGGATCAGGCTCTTCCTTTTTTTCCGGAGTTCCCTG5530.1039893942100661No Hit
GTGCCTTGTTAGAAGAAGCGGAGCAATTGCTAGACCGAGGCATTCACCCA5530.1039893942100661No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT5360.10079261355623044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)