FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729551
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC506346.940433225367383No Hit
TTAATGATGTTGTCAGGAGCTCTGTTCATGTTGAGGTTCTTGATTCTCAC199112.7292128994408893No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA139971.9185773167331686No Hit
CACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCATTAC67860.9301611539152164No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG60330.8269469852004864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.6974152595226378No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA44380.6083193635537475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42510.5826871596365435No Hit
GAAGTGCTCCAGAAGCTGCAGGTGGGAAGCCCATCACCATGACACTGGGG33360.4572675522341824No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG32940.4515105866485003No Hit
GGTTTACTCCTTCAGCACCACAGGAAAAGAGTACAAACACATCCCAGACA32720.44849503324647627No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC32150.44068200852305045No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA32130.4404078673046846No Hit
GTTGAGCACATCAATAAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACT31660.433965548673088No Hit
GTCCCAGCACCGGTGCCACCCTCCTAAGCCCCGGGCAGGCAGTACGTACA31140.42683787699557674No Hit
TGCTTCCGGGGCAGGTGTTGGGCCCGCTGGGGCTGGGCCAGGAGTGAGCT29890.40970405084771316No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC29560.40518072074467715No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26530.36364832616225595No Hit
GTGTTTCAAGTGCTCACCCCCATGCCTCTCTCATCCCACAGAGCCACAGT23960.3284211796022485No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23390.32060815487882277No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT23230.3184150251318962No Hit
TACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCATTAC22590.30964250614419003No Hit
CTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGC22400.30703816456971483No Hit
TGTTTCCGGGGCAGGTGTTGGGCCCGCTGGGGCTGGGCCAGGAGTGAGCT19710.27016617069951243No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT19580.2683842527801346No Hit
ATCTTCTTCAGTCGCTCCAGGTCTTCACGGAGCTTGTTGTCCAGACCATT19280.26427213450464737No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18440.2527582033332831No Hit
TACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTAAACGCC18330.2512504266322711No Hit
TCTTAACATTGAAGCCGATTTTGTTATTTTCTGCCCTGAACCAGGGCAGA18000.2467270965292351No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA17920.2456305316557718No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC16990.23288296500176137No Hit
TCTTCAATCTGATCATCGGTCAGTCCTTGCATATTAGGAGGGAGAAATAT16780.23000448220892028No Hit
GTTTTCACCAGTGGCAACAGCCAGTTACAGGCTCCTGCCAGACATCACCC15830.216982774336544No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC15510.21259651484269093No Hit
TGCATCGTGTTGGCAGCAGGTGCCTCTCAGCTGGTGCTGGACAGAAGGGG15300.20971803204984985No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA14930.20464641951008222No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14700.20149379549887536No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14260.19546268869482736No Hit
ATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTTTTATCTCCGTC14200.19464026503972992No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13720.1880608757989503No Hit
ATAAGAGACAGCCTCCGCTCCCGCTCTCTGTCGGTGGGCGCGGGGGAATC13620.1866901697071212No Hit
GCCCAGGAGAGAATGTCCCAGAAAGAACGGAGCCCCATGCTGCTAAGAAT13610.18655309909793832No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13340.1828521926499998Clontech SMART CDS Primer II A (100% over 21bp)
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC13220.1812073453398049No Hit
GTCAGAATACATGGCTGAATACAGAGTTGAAAACCAAAACTGATGAACTT12500.17133826147863548No Hit
AACGCACACCGGCCTTATTCCAAGCGGCTTCGGCCAGTAACGTTAGGGGG12490.17120119086945257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.16434766041030716No Hit
AGACAGTACAGAGGACAAAGACCCCTCACGATGAATGCTCTTCAGCCAGC11980.16421058980112424No Hit
TAGATGTACAGCTTGCAAGTAGAAGTCTCAGGCAACAGATTAAACCAGCA11780.1614691776174661No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11670.15996140091645408No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11630.15941311847972245No Hit
CTCTTATTTTCAGAAGGAAAGAAGGGAGGGCAGAAGGATGAAGGTATTTG11570.158590694824625No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11560.1584536242154421No Hit
CTTCTGTGAGGTCTGCATACCTGCGATCCTTACTGATGTTGCTGTAGAAA11140.15269665862975995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10770.14762504608999233No Hit
GTGCCAAACCAGGAAATAAACAGCAACACTCTAGCGAAGCCACGCCAACC10610.1454319163430658No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10540.14447242207878544No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10510.14406121025123672No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10390.14241636294104182No Hit
TCGCCAAAGTGACAGGCGGTGCGGCCTCCAAGCTCTCTAAGATCCGAGTC10380.1422792923318589No Hit
AATCTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAAC10130.13885252710228618No Hit
GTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACTCCAAT9990.13693353857372548No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9570.13117657298804333No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9570.13117657298804333No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9520.13049121994212878No Hit
CAATCCTAATGTCCCCTGATATGTCTCTAGCGACTTGACCATCTCTTGTT9370.12843516080438516No Hit
CCTCCTTCACCGAGAGAGCTTCTAGCTTTTCCGCCACTTTTTCGGCATGA9200.1261049604482757No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9090.12459718374726372No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA9000.12336354826461755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8960.1228152658278859No Hit
GGCGTGGGTCACGCTCTCGCTCGGTCTTTCTGCCGCCATCTTGGTTCCGC8940.12254112460952012No Hit
ATGCAGGACCTCTGCTCTGGGGCAATTGGGCTGGACACTTTCCGTGGCGT8870.12158163034523975No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8840.12117041851769102No Hit
CCGCAATGCAGCCCCAGGTAGATCTAGGGGCAAACGGTAAAGGCGCTCCG8760.12007385364422776No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC8240.1129461819667165No Hit
GCATGGGAGGGAACAAGGAAAACATGGAACCCAAAGGGAACTGCAGCGAG8030.11006769917387543No Hit
TCGCAATGCAGCCCCAGGTAGATCTAGGGGCAAACGGTAAAGGCGCTCCG7810.10705214577185146No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7740.1060926515075711No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG7580.10389952176064457No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT7520.1030770981055471No Hit
AGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTC7410.10156932140453512No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC7310.10019861531270603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)