FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663979
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7298910.99266693675553No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA192452.8984350408672563No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG90661.3654046287608494No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA63510.9565061545621171No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG47400.7138779991535877No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC41050.6182424444146577No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG37530.5652287195829988No Hit
GGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAATGCTGTCAA37180.5599574685343964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34480.519293531873749No Hit
GTCATTTCCTGAATGCTCTTCATCCTCCTCTTCATCCCTGAAGATGTCTT34390.5179380673183941No Hit
GGCCAGTCCTGAGCAGGCCCAACTCCAGTGCAGCTGCCCACCCTGCCGCC33970.5116125660600712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27700.41718186870367885No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC25720.3873616484858708No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT24280.36567421560019214No Hit
TGGGAATAGGGAAGGATGAAGAGAAGGGGTGGATGGGATGGGGGCACCCC23500.3539268561204496No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21850.3290766726056095No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC20830.31371474097825386No Hit
TATTGCAAGAGCTTGACTCCTGGTTGCCTTTTCCCAGCCAGTTTTCAGTT18990.2860030211798867No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18200.2741050545273269No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC18130.2730508043176064No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA16430.2474475849386803No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15890.23931479760655078No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC15530.23389293938513114No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14900.22440468749764675No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT14890.22425408032482955No Hit
GTCTCTGGTGTTTGTAAGAGGTGGTATCGCCTAGCGTCTGATGAGTCTCT14880.22410347315201234No Hit
GAGGAACCAGATGGCTGAACCCCCGCCCCCAGAGCCCCCAGCAGGGCCCT14800.22289861576947462No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14290.21521764995579679No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA14270.21491643561016238No Hit
GGCAGAACCCAGGGCATGAAGATCCAAAAGGGCCTGGTTCAGCTTTTTCT13540.20392211199450586No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC13380.20151239722943048No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT13310.20045814701970996No Hit
GGAAACATGACAGCGGTGCGGCGGACTTGGAGCGGGTCACCGACTATGCA13180.1985002537730862No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12880.19398203858856983No Hit
TGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAATGCTGTCAA12430.18720471581179524No Hit
TCTCAGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCCATTCTCCTGCC12050.18148164324474117No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12010.18087921455347233No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11990.1805780002078379No Hit
GGATAGCTCCAGTCCCCAAGCTGTGAGCGCACGAGGAAACTGCACTGACG11800.17771646392431087No Hit
GCATCACTTCAAATACTTTATCTGTCTTGATGATTCCATTTTTGCGGAGA11550.17395128460388057No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC11390.17154156983880514No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA11350.17093914114753628No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT11130.16762578334555764No Hit
GAGGAGAGGCACCCGATATATGTTCTCTAGGCCTTTTAGAAAACATGGAG10810.1628063538154068No Hit
TGGTGACAAAGACAGTTTGGCTGGGGGAATCCTGGGGGCCAGCACCCCCC10650.1603966390503314No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC10370.15617963821144945No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA10180.15331810192792242No Hit
TGCCAGTCCTGAGCAGGCCCAACTCCAGTGCAGCTGCCCACCCTGCCGCC10080.1518120301997503No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10000.1506071728172126No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC9910.14925170826185769No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9340.14066709941127656No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAT9250.13931163485592166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.13690192009084626Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGCAACCAAGCCCTCCAGCAAGGATTCAGAGTGCCCCTCCGG8910.13419099098013643No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8900.1340403838073192No Hit
GTAGAGATGGGGTTTCGCTGTGTTGGCCAGGCTGGTCTCAAACTCCTGAC8870.13358856228886756No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC8570.1290703471043512No Hit
CAGCAGAGACGGCTACAGCCCCACCAGCAGGTACACTGGCAAGCTTGCCA8550.12876913275871676No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG8520.12831731124026513No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8510.12816670406744793No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8350.1257569893023725No Hit
AGATCAACGCGAGCAAGAATCAGCAGGATGACGGTAAAATGTTTATTGGA8260.12440152474701761No Hit
GATTCAACCCAATGCATTTACCACCAGGTTTTCTGCCTCCTGGACCCCCA8170.12304606019166268No Hit
TAGAACCCAGGGCATGAAGATCCAAAAGGGCCTGGTTCAGCTTTTTCTCC8140.12259423867321106No Hit
GCTGGGATTACAGGCATGTGCCACTGCACCTGGCTAATTTTGTATTTTTA8130.12244363150039385No Hit
AGCTCAGGGTGTGCCTCGGCCGCCAAGTGCACCCCAAGGGGGACTTTGGG7900.11897966652559795No Hit
GCCCCGGGGTCAGGGCCCTCCCACAGCATTCCAGCTCCCTGCAGGTCAGG7840.11807602348869468No Hit
GGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCT7800.11747359479742582No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA7790.11732298762460862No Hit
GGAGACGGGGGCCCGAGGGAGCCTGCCCCACGGCAGGCCCATCACCTGCC7750.11672055893333975No Hit
GGGCTATTGGACTCTCTGTAGCAGATTTGGCAGAGAGTATAATGAAGAAT7620.11476266568671599No Hit
GGCCCTCACTGGGGCAGCTGGAGGAGCACGGACTGCCCCGCTGGCAGGTA7520.11325659395854386No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC7500.11295537961290944No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7500.11295537961290944No Hit
AGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAATGCTGTCAA7300.1099432361565652No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT7160.10783473573712421No Hit
GGAGACGACAGGTTTCCAAAGCTGTCAAAAAATCTCTGGGTCCAGGGGTA7120.10723230704585537No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT7100.10693109270022093No Hit
GGAATGAAGGGGCCTCGAATGCAATAGAATGGAAAGGAATAAGCTCGACT7020.10572623531768324No Hit
TTGCTACCCAGTATATGCAGCACTTGTGAACTCCCAACAGGTTCCCAGCT6860.10331652055260783No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6840.10301530620697341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.10301530620697341No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG6820.102714091861339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6800.10241287751570456No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC6790.10226227034288735No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC6700.10090680578753244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)