FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683517
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT396235.796929703284629No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA166852.441051210138153No Hit
ACACAGAGTAACCACCATGGGCTTTGGCGACATTGTTGATTAACTCCATG88301.2918478984429063No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA62560.9152661894290851No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG57520.8415299107410642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38330.5607761035936194No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC34600.5062054052788738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32080.46933726593486336No Hit
CATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAAC30900.45207361338489027No Hit
AGATTTACAGAATGTCAACATCACACTGCGCATCCTCTTCCGGCCTGTCG27260.3988196343324307No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25780.3771669175748372No Hit
CAACAGATGTCTGGGCTTAAGGGAGTTAAGTCTATGCTGAAAGATGGAAA24860.3637071206714683No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24240.3546363879757197No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC23550.344541540298193No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22400.3277167941689819No Hit
ACTACAACCTCCACCTCCAGAGTTCAAGTGATTGTCCTGTCTCAGCCTCC20950.30650298383215047No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC15940.23320561156489156No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG15550.22749982809498523No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15420.22559790027168306No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC14930.21842909539923658No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13460.19692268078189715No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13370.19560596151961107No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12840.18785194808614855No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT12330.18039053893319407No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT12320.18024423679294005No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12200.17848861110989192No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT11750.1719050147984615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11620.17000308697515937Clontech SMART CDS Primer II A (100% over 21bp)
GTGCCAATCGTGGTCCTGGTGTGGACTACTGTGGGAGCAGGGGTCTCCAC11590.16956418055439731No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11350.1660529291883011No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10970.16049344785864872No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT10770.15756740505356853No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10700.15654329007179046No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG10620.15537287294975838No Hit
CCGCAAGGTCGCTTTGCAGAGCGGGAGCGCGCTTAAGTAACTAGTCCGTA10320.15098380874213807No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10080.14747255737604187No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA10020.14659474453451782No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9920.1451317231319777No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC9850.14410760815019963No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA9790.14322979530867558No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG9220.13489057331419702No Hit
GTGGAGGACCTGGATATGGCAACCAGGGTGGGGGCTACGGAGGTGGTTAT9220.13489057331419702No Hit
GAGCTGCAGGTTGAATCCGGGGTGCCTTTAGGATTCAGCACCATGGCGGA8800.1287458834235286No Hit
TTCCGGGAGGCAGCGGCCGCAGCGGCCTCGCCATGTCCCAGCCCGGCCAG8700.1272828620209885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8680.12699025774048048No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC8510.12450312135616232No Hit
GGTCCCGATCCCCGGTCCTCAGGTGCTTCCACTCAGGCTTGAGGCAGGAG8350.12216228711209817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.12201598497184415No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8270.12099186999006609No Hit
ATATAAAACCAGCTTGCGTTGTTTCAGCTTGTCTTCATTTAAACTTGTGG8030.11748061862396986No Hit
GCAAGGAGTGCATCTCTGCCCGGCCGCCGCACCGTCTGGGAAGTGAGGAG7630.11162853301380947No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7560.11060441803203139No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7450.10899509448923728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7350.10753207308669718No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG7250.10606905168415709No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT7190.10519123884263303No Hit
GCTCGGGACAACAAGAAGACGCGCATCATCCCTCGTCACCTCCAGCTGGC7160.10475233242187099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)