FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150214
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG65974.391734458838723No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11210.7462686567164178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.7242999986685662No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10320.6870198516782724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.553210752659539No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7050.46933042193137786No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT6530.43471314258324795No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC6420.42739025656729734No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6050.4027587308772817No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC5070.3375184736442675No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA4830.32154126779128445No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT4700.3128869479542519No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC3880.2582981612898931No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT3880.2582981612898931No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.2523067090950244Clontech SMART CDS Primer II A (100% over 21bp)
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA3360.22368088194176308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.21169797755202577No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG2940.1957207716990427No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT2890.1923921871463379No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG2880.19172647023579692No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG2780.18506930113038733No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC2760.18373786730930539No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT2700.1797435658460596No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC2640.17574926438281385No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC2460.16376635999307654No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC2450.16310064308253558No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG2440.16243492617199462No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2290.1524491725138802No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC2260.15045202178225733No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA2150.14312913576630673No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC2140.14246341885576577No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG2070.13780340048197903No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT2040.13580624975035616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2010.13380909901873325No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC1930.12848336373440558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1840.12249191153953694No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT1830.12182619462899597No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1670.11117472406034058No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT1590.10584898877601288No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG1590.10584898877601288No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA1580.10518327186547193No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAA1510.10052325349168519No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC1510.10052325349168519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)