FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651477
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC603619.265254183954307No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA147762.268077000415978No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG77771.1937489734864009No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA54350.8342581549310261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.6018631509631193No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38780.5952627644567652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.48735411994590755No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31490.4833631885699725No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC31350.48121422552139215No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC22360.34322009833040923No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21070.32341893881134715No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC20190.30991117107741334No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19760.3033107845710593No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC19050.29241247196754455No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18150.27859770951238494No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT16360.2511216819626787No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG16000.2455957769806148No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15320.2351579564589387No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC14500.2225711728886822No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14430.22149669136439198No Hit
GTTCTTGGTCTTACTGACTTAAAGAATAGAAGCCGCTGACCCTCGCGGTG13680.20998438931842567No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12960.198932579354298No Hit
GCTCCGAACAGTTTCAGTTGCAAGAGTCAGAGCACTGACTGATACCAACA12840.1970906110269434No Hit
GGTGCAGGCTGAAGCAGCGCCTCAACAGCCAGGGACATGTAGGCAACACG12340.18941574299629918No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12300.18880175355384765No Hit
TTGCATAGTTAGGGTCAGGCTGGGCAACTTTGACGCCTGGCTTTGGGACA11920.18296885385055803No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11790.18097338816259056No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC11710.17974540927768748No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC11510.17667546206542978No Hit
GATTTGGTCGTATTGGGCGCCTGGTCACCAGGGCTGCTTTTAACTCTGGT11470.17606147262297825No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11090.17022857291968865No Hit
CTTGACAAAGGTGGCGTAGGGATCGTCGACAGTCTGGCCCACATCGCCCA10490.1610187312829156No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC10390.15948375767678674No Hit
TCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAA10370.15917676295556096No Hit
GATCAGGGGTTAGAAGACATAAACCAAGTCTGCCCAGTTCCCTTCCCAAT10330.15856277351310946No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC10300.15810228143127078No Hit
GTGCATCGCGGTGATTGCCAAGGAGAATTACCCCCTCTACATTCGCAGCA9970.1530368685310456No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9900.1519623870067554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.15134839756430388Clontech SMART CDS Primer II A (100% over 21bp)
CCTTGAGGCCTCCTAAAGCCTTGGGTCCCTCCGCCCCACCCCTGCCCAGG9780.15012041867940082No Hit
GAGTACATGGGTATCCCGGCCCCTAAGCTGCCCAATTTCGTTCACCCTCG9630.14781795827020755No Hit
CTCTCTGCCATTCATTCTGCCTGATGCTAACAACACGCAGCTGATTTAGG9610.14751096354898177No Hit
CCTCAGGGAAGGGGCAGCACATGCATTTGCCCAGTATAACATGGATCAGT9300.14275254536998236No Hit
TTGTCAGAATCCTGGTGACCGATCAGATCTTCTACCCAGTGAATATAGTT9300.14275254536998236No Hit
GAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGAT9140.14029658760017621No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9080.1393756034364989No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG8970.13768713246975717No Hit
CTCCAATTATTCCAAGTTTGACTGGAATAACCCTTCCCATCAATACATCC8970.13768713246975717No Hit
GATAAGGAGCTGCCCATCTCGGGCTTCATGGGGCCCCTGGACTTCCACAT8960.1375336351091443No Hit
GTTTGAAGACCCTGGAGCAGGGGGAGCCCTGGAGAAGTGAAAGATCACTT8930.13707314302730564No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC8910.1367661483060799No Hit
TAGCAGGACAGTAGTGGACACTGCGGACGACTTTGGGACGAACTAGAGAA8800.13507767733933815No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8650.13277521693014488No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8610.13216122748769335No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8540.13108674596340317No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8440.1295517723572743No Hit
GTACATGGGAAAAGAGTCTCTACCCCTTTCTGTTCGAAGTTCATTTTTAT8310.1275563066693068No Hit
GGTCATGACAGTGAATTACAACACGCATGGGGAGCTGGGCGAGGGCGCCA8290.12724931194808103No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8250.12663532250562953No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT8240.12648182514501663No Hit
GACCTCATAAGGCCACGTCCTGAGAGCCAAGTGGTGCTCAGAACTCGACC8240.12648182514501663No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8080.12402586737521049No Hit
GAACAGGCCTAAAAGGCTCATCAGATTCTTTCCACCTAGAAAACAGGAGA8080.12402586737521049No Hit
GCTGGGACTACAGGCGCTTGCCACCACGCCTGGCTGATTTTGTATTTTTA8070.12387237001459761No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8040.12341187793275894No Hit
GAGCGCGAAAAAGCACGTGGGCAGAGCTGGCAGCGGAAGGGGCGCTGCCC7970.12233739640846876No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC7920.12156990960540434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.1209559201629528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7840.12034193072050126No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7730.11865345975375953No Hit
GCTCACTGCAGCCTGGAACTACTGAGCTCAAGTGATCCTTTGGCCTCAAA7710.11834646503253377No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC7700.11819296767192089No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7510.11527651782027608No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC7500.1151230204596632No Hit
AATTTCACGAGTACCTGATCACTGAGGGAAAATGCACACTGGAGACTAGT7470.11466252837782454No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7290.11189957588679261No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG7280.11174607852617975No Hit
GCCTCAGGCCCCGGCTCCCTCCTGAGCTGCGCCGTGCCAGGCCGCCCGCC7200.11051809964127667No Hit
AGAAAAGGGCTGGCTGTAGTTGTATAAGTTGAGGTACCTTTGGCAGTTGT7140.10959711547759937No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG7130.10944361811698648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7020.10775514715024474No Hit
GGCCTGGCCTGATCTCAGGCTGGACTGTGGTGTGGGCCCCTTTGAGTGAC7020.10775514715024474No Hit
GGCCCGGTCCTAAGCTGGACTCCTCCTACACAATTTATTTGACGTTTTAT7010.10760164978963188No Hit
TCTTCCATGACTTTGGCAATCTGGGCTGCAGTGGTGGAGATCTGCAAACC7000.10744815242901898No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG7000.10744815242901898No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG6960.10683416298656744No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6940.10652716826534168No Hit
CAACCAAGCTGTGATCCGGGGGTGTCCGAATGGGGAAACCCAGCTGGAGT6860.10529918938043861No Hit
GTCTGCAATTAAAGCGTTCTTGGCATCCCAGTCTTCTCATCTCCTTGAAG6850.10514569201982571No Hit
CTTTATGAAAGGTTATTTCAGGAACAGCGGAAGGGTTACTGATCCCGGGA6790.10422470785614842No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6750.10361071841369687No Hit
CCATCTAGAGTGAGCAGGCTCAGAACAAATGCATCCAGTCCTTTAACCTC6720.10315022633185823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)