FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769171
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT432735.625927134538354No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA118071.535029271774417No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG57600.748858186281074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49630.6452401351585018No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA46550.60519702380875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.5448723365805523No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA41410.5383718314913069No Hit
CACCAAACCCTCTGTGACTTTACTGAATTCTTCCAAAATGTTTTCTTTAG37200.4836375786398603No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA31770.4130420933706549No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA29880.3884701841333072No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27990.36389827489595944No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC25840.33594610301220407No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC25250.3282755070068944No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22830.29681306237494653No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC21130.27471134507151207No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18720.24337891054134905No Hit
GAAAGATGGAAAACCAGTGCAGCCTGTCAAGCGAGAGCTTTTACGGCATA18170.2362283549431791No Hit
AAGGACTACGGCAACTCACCCCTGCACCGCTTCAAGAAGCTGGGCTCCAA16510.21464667804688425No Hit
GCAGTAACTGCAGTGGGAGAGAGAACCTCCTCTTCTTTCTGGCACCAAAA15840.20593600122729536No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA15800.2054159608201557No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA15410.20034556685054428No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT15180.1973553345094914No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC14400.1872145465702685No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT13850.18006399097209852No Hit
GCTCAATATTTGCATGTACAGTCAACCTCAGGCAAATCAGGCAGAGGCCT13710.1782438495471098No Hit
GAGTTGGTGCTGGTGGTTGTGGTGGCAGATGATATTACCTGAAGAAGGGA13670.17772380913997018No Hit
GTCTTGAAGCCCCACTTCCACAAGGACTGGCAGCGGCGCGTGGCCACGTG13580.176553718223906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.1738235060864229Clontech SMART CDS Primer II A (100% over 21bp)
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13360.17369349598463799No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC13150.17096328384715492No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13060.16979319293109074No Hit
GTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA12780.1661529100811133No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT12630.16420275855433966No Hit
GTACATGGGACGGTGAAACTCCGTCTGTACCAAAAATACAAAAAAATTAG12400.16121252621328677No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11810.15354193020797716No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT11380.14795149583122608No Hit
GTTGTGCTCAAACACCTTGGCTGTGCTCAGATATTTAGGCCTATCTGGGT11290.1467814049151619No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11220.14587133420266754No Hit
CTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAAT11120.14457123318481846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.14418120287946373No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10960.14249107155625992No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT10660.13859076850271265No Hit
CTTCTGACCCCTGTGGCGTGCGTCCTGGCGCGCCGCACCCGCCTGGTGCC10520.13677062707772394No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10500.13651060687415412No Hit
GTAATATGCTTTGTGGCATCTGGAGCACGTTTTGGGGCCTAAACAGCCAC10400.13521050585630504No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG10390.13508049575452014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10330.13430043514381068No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10160.13209026341346722No Hit
GGCTTGGAGCTCATGCGCCCTGGCTTCCTGTTGAAGTCGACCTGCTGGAA9990.12988009168312378No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT9850.12805995025813505No Hit
GAAAGTTCCTATCTGCCATCTAGATCAAGAGCCTGAACTACAGTATTTTC9840.12792994015635015No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9770.1270198694438558No Hit
GATTGTTACTGCTCATTCATGGAGAGTGTTGGGAAAAGCAGTTTTTTTTT9730.12649982903671617No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9450.12285954618673871No Hit
GTCTGGGGGAGCAGAGCCAGGGGCAGGTGTGTCTTCAGGAGTCGTGGGGC9370.12181946537245944No Hit
AGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACA9370.12181946537245944No Hit
CAGTGAAGCCAGCTGCTTCCATTGGTGGGTCATTTTTGCTGTCACCAGCA9350.12155944516888963No Hit
GTGTGGACCGTCACACCAGGAAGTTCAAGGACAACACTGAGCCTGGCCAC9300.12090939465996507No Hit
GCTTCGCGGACCCCGGGTGCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTC9300.12090939465996507No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC9290.12077938455818016No Hit
TACCAAACCCTCTGTGACTTTACTGAATTCTTCCAAAATGTTTTCTTTAG9240.12012933404925562No Hit
AGCATGGCCGTCTCCCCCTCGTTGATGTGTTCTGACGACTTCACAGCCTT9210.1197393037439009No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9150.11895924313319146No Hit
GCATGAAGGCTGTGTTCCAGACTCCTCCTGCCCCCAATCCTCTGTGCCCC9110.11843920272605181No Hit
GTTTTTTCCTCACTGAACATGTTTTCCTCACTCACACTGAACATTTGTCT8990.11687908150463291No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG8920.11596901079213855No Hit
AGTGGGGAGAGATAAGGTACCTTTAAAAAGAAATCAAGGAAAAAGGGAGA8880.11544897038499892No Hit
ATTACATTGCTTCCAGCTCTTTGAAAATCGTTAATTCAACTGCAGGTAAG8730.11349881885822528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8720.11336880875644037No Hit
CCTGAGTAATTCCCACATTCGTACTGGAATTTTGCATATCTGTAGAGTGT8690.11297877845108564No Hit
ACTCGGGAGGCTGAGGCAGGACAATCGCTTGAACCCGGGAGGCAGAGGTT8640.1123287279421611No Hit
GGCATAGCCACCGTGCCTGACCAGGGCTCTTTTAGCAAGGAAAACGTGAG8500.11050858651717238No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC8450.10985853600824784No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT8230.10699831376897984No Hit
GGATTACAGGAACCCACTACCACACCCAGCTAATTTTTAAATTTTTAGTA8190.10647827336184021No Hit
AAGCAGGAGGCAACAGTGAAAGTATTGAATCCTGGCTTTCTTCTTTCATC8130.10569821275113077No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8040.10452812183506657No Hit
ACTTCTACCTGGTCCCCCTTCATTACAGGTTGGCCGTTGTCCAATGTGTT8020.10426810163149677No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8010.10413809152971186No Hit
ATCCTATACAATGAAAGCTATAGGTTTGCTTTTGTTTACTTGTTTTTGCT8010.10413809152971186No Hit
CTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTA7980.10374806122435713No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG7950.10335803091900242No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC7890.10257797030829297No Hit
CGTCTACTCCTGAGGACAAGGAGAGTGGGCAGGAGGGGGTCGACAGTATG7880.10244796020650805No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG7850.10205792990115331No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7770.10101784908687404No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT7760.10088783898508913No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7760.10088783898508913No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC7730.10049780867973442No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7720.1003677985779495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)