FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664228
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA192582.899305660104663No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA58380.8789150713309286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.7182774589448202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.6332163052445847No Hit
GTGCAGAAGCGCTTCGGGTGAATTCATACCAGAGCCACCGGGTGTGACTC41130.6192150887948114No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC33560.5052481979079473No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA33040.49741956075323535No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG24350.36659098984083777No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT21280.32037192048513463No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA20350.3063707040353613No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18950.2852936040034446No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA18520.2788199232793559No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC18350.27626056113262315No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT18140.2730989961278356No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC15500.23335360749622117No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14830.22326670962380385No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC13560.2041467688805651No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.19917859530161328Clontech SMART CDS Primer II A (100% over 21bp)
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12810.1928554652920383No Hit
ATACTACGTCGATGACCCTCCCCGCATAGTCCTGGACAAGCTGGAACGCA12430.18713453814051803No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC12400.18668288599697694No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11920.17945645170031976No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC11870.1787036981277513No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11660.1755421331229638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.1669607423956834No Hit
GAGCCACTTCTTCCGCGAACTGGCCGAGGAGAAGCGCGAGGGCTACGAGC11030.16605743810860127No Hit
GAACCAAAGGCCTCCTGTCCAGCTGCTGCACCCTTGATGGAGAGAAAATT11000.16560578596506018No Hit
GCACCTCAGGGGTGAATTCTTTGCCGAGATGGATTGCCAAAACGGTCACC10790.16244422096027267No Hit
GCCTCAGCCTCCCAAGTAGCTGGGATTACAAGCATGTGCCACCACTGCCA10480.15777714881034824No Hit
GTGCAAAGATGTTCCCTTTCAAGTTAAGTGTGTGGCCTGCCACAAGACAC10260.15446503309104706No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10080.15175512022980062No Hit
GTGACTGTCTGGAACCGCACTGCAGAGAAATGTGATTTGTTCATCCAGGA10080.15175512022980062No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9880.1487441059395268No Hit
GTCTAAGGAGTTCATAGAATTTCTCAAGACCTTCCACAAGACAGGCCAAG9720.14633529450730773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9710.14618474379279406No Hit
GCCCCCTCAGGGCACTGTGACTGCAGGGGCAGGAATCGGCAGCAGGCCCC9600.14452868593314344No Hit
CTATTGATGTAAACCGAGAAGCCATTATTTTGGAAGCCAAACTCCTTTGC9580.14422758450411605No Hit
GCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGAC9390.14136712092835593No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC9110.13715170092197257No Hit
ATATTAAACTATTTCAGCACCAGAAAAAACTCAATGGGTATTAAGAGACC8790.13233407805753447No Hit
GTATACAACATGGGTTCTACAAAAAGTGTCACCAATCATCTTATGTACGA8720.13128022305593864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8640.1300758173398291No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT8330.12540874518990466No Hit
GAGGAGAAGGACCCTCTGAGCTATTTTGCGGCATACGGGAGCAGCAGCTC8270.12450544090282253No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8270.12450544090282253No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8210.12360213661574038No Hit
TGTTGGGGTCAAGTCGCCGCACGATCTCCTCGATGCTCTTGTGCTTCTGG8210.12360213661574038No Hit
GGGAGGAGCCTCTGCAGACACGGCTTCCTGCCGAGTATCCAGGACCGGGC8070.1214944266125487No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA8050.12119332518352131No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7870.11848341232227488No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT7810.11758010803519274No Hit
GATCCGCTCGGCGTCGAAGTCGATACGAGTGTGATCGGCACGGGCGGAAG7740.1165262530335969No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7640.11502074588846No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT7620.1147196444594326No Hit
GAGTGCAAGATAGAATATGATGATTTCGTAGAGTGTTTGCTTCGGCAGAA7410.11155807945464509No Hit
ATCCAATTCCGACACACGACTTGTCACTACTCCTCTCCCCTTGAAAAAAG7370.11095587659659031No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7280.10960092016596712No Hit
GGACTAGATAGGTCTTGTCAACTGTATGCTCAGTCAGGAATGTCTCTGGA7240.10899871730791234No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA7210.10854706516437128No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC7190.1082459637353439No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG7040.10598770301763852No Hit
CTCCAGACCTTTAGGCCGAGGCCTGCCAGTCTCTGGACGGCTACGGCGTA7040.10598770301763852No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG6830.10282613801285102No Hit
TCCCGGATTTACCTAAGATTCCAGCCTACCACCTTAAACTTGGACAACCA6790.10222393515479625No Hit
GGTCACGTGGTCACCCAATTCTTTGATGGCTTTCACCTGCTCATTCAGGT6650.10011622515160457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)