FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567296
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT518969.147958032490974No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA144392.5452321186823106No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG65421.153189869133574No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48090.8477056069494585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.6374097472924188No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG35770.63053502933213No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC34260.6039175315884476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31340.5524452842960288No Hit
ATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACC27670.4877524255415162No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26600.46889101985559567No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC24390.42993428474729245No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21330.37599418998194944No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA18030.31782349954873645No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT17780.31341662906137185No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15110.2663512522563177No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14470.25506966380866425No Hit
GCTGTGGCCAGGAGAGCAGCAGTGCCCTGAGCTGCCCTACCGTCTCGGTG14380.253483190433213No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC14220.25066279332129965No Hit
ATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATG13610.23991002933212996No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC13300.23444550992779783No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13280.23409296028880866No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC12830.22616059341155234No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12790.225455494133574No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT12560.22140117328519854No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT12330.2173468524368231No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11650.20536016471119134No Hit
GACGGTAGCCCGAGGACGATGAAACAGGTTACCCACTGCGAGTCCTGGCC11510.20289231723826717No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11070.1951362251805054No Hit
GCAGAGGGTAGGGAGGAAGCAGTTCTTCCAATCCCAGTAGATGCAAAAGT10860.19143445397111913No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10520.18544111010830325No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10200.17980031588447654No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC10180.17944776624548736No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.1764510943140794Clontech SMART CDS Primer II A (100% over 21bp)
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG9890.1743357964801444No Hit
TCACATGAGTCTCCCAAGTAGTTGGAACACAGGTGCCAGCCACCACACCT9870.17398324684115524No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA9520.16781362815884476No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9430.1662271547833935No Hit
GTACATGGGGGGGGCGGACCGGCGCTGGGCAGCCAGGACAGCCGCGGCAG9290.1637593073104693No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9240.16287793321299637No Hit
GGCCAGGCCGCCATTGGGATCGGTCAGGCCCAGCAAGTCCTCTGCCAGGC9160.1614677346570397No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8810.15529811597472923No Hit
GTTTATGCAGCAGGCTTCTTCTCCTCTGTAGTAGGTTTCTTCTCTGCAGG8300.1463081001805054No Hit
GTACATGGGCCACCCCACAACAGTCCCCAGAGTGTGATGTTCCCCTTCCT8210.14472162680505415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.14348770306859207No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8020.14137240523465702No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7850.1383757333032491No Hit
GAGGAAACGGGAGTGAACGGAGAGCGTAGTGACCATCATGAGCCTCCTCA7790.1373180843862816No Hit
TGCTTTGAGTGCAGGGGTCTCTCTTTTTGGCATGTCGGCCCTGTTACTCC7780.137141809566787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7550.13308748871841156No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7540.13291121389891697No Hit
AAGATTACCTTAAGCAACACATGAAAACTCATGCCCCAGAAAGGGATGTA7360.12973826714801445No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG7250.127799244133574No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA7160.12621277075812273No Hit
CTCCAATTTACTGTTGGATCATCCATCTTATTAATTAGCACAATTAGGTG7000.12339237364620938No Hit
GCGTAAGTGTGGTGATTTGGATACCAATCATAAGTCTCCTTCTTCTTGGC6950.12251099954873647No Hit
AGGTGACCCATCTGGCCCGCCGCGATGCTGGCAACACGGCGGCTGCTCGG6940.12233472472924188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6860.12092452617328521No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC6760.11916177797833935No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6620.11669393050541517No Hit
GTACAATGTCTTAGAATCTAGAATATAAAGGTTGCTGGTCCTGATCCCTT6520.11493118231046932No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6520.11493118231046932No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT6480.11422608303249097No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT6360.11211078519855597No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC6260.11034803700361012No Hit
ATTCTCTACTGCCGCTGATGGTCAAACGCAAGTGGAAATTAAAGTGTGTC6130.1080564643501805No Hit
CAGTGACCTTAGCTACATAGCAGACTTTCCCAAATGTATTGATTACAAAT6100.10752763989169677No Hit
GGGCAGTGTCCTGAGCTCTAAAACTTCGAAATCTGACCAGGCACAGTGGC6080.10717509025270759No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6030.10629371615523465No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCAT6030.10629371615523465No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5800.1022393953068592No Hit
GTTGGCCTCATAGTCATTGAATTCTTCCTCCTCGATGTTGTGGGAGACGG5760.10153429602888085No Hit
GTGTTCTAGGCCGAGGAGACAGCTAGAGCAAAGCCTTGTGATGGGACAGT5750.10135802120938629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)