FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529547
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA229554.334837134380896No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA59791.1290782499003864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42410.8008731991683458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.7632939097001777No Hit
GTCCTGAACAACCACACCAGCCCAGAGCAGTCGCCCTCCACTTTTCTCAG33640.6352599485975748No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC31980.6039124005990025No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26560.5015607679771579No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26130.49344062000162403No Hit
TCTCTGCTGAACTGCCCTCACCCCATGGGCCAACTTCTGCCCCCCTTACC22400.4230030573301331No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA20020.3780589824888064No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA19970.3771147792358374No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT19260.36370709304367693No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT17990.33972433041826317No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16870.3185741775517565No Hit
GCCCAGGACCCACCCTCGCTTCTTGGCGTAGTCTGTCATCTTTTTGGGTG16610.31366432063631744No Hit
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGG16300.3078102604679094No Hit
GCTCCAAGGGGTGGGGGACTTTGTAGCCAGCAAATAGCACTTGCGGGTCT15700.2964798214322808No Hit
GCAGTAATTCCAGGGTCATAGGAATATTCTTAAGCTCCTTTAGCAAATCC15560.2938360523239675No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15340.2896815580109036No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14790.27929532222824416No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC14390.27174169620449173No Hit
ATTCTTCCCGGGCGTAGGCATTGCTCAAGTACCGATGCACTCCCCGGAAG14270.26947560839736606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.2405829888565132Clontech SMART CDS Primer II A (100% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12650.2388834230011689No Hit
GGTGAGAAACCCTATGCATGTCCGGAATGTGGGAAAGCCTTCATTTCTCT11990.2264199400619775No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11920.22509805550782083No Hit
GTACATGGGGATGTTTTCTAGACACAACAAAATGTGGGAGGCACATGTTA11890.22453153355603941No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG11700.22094356119475703No Hit
GAAATATTCTTTGCTCTTTTGGACATCAGGCTTGATGGTATCACTGCCAG11330.21395645712278608No Hit
ATCCAGTCCATCATGGCCATTGTCAAAGCCATGGGCAACCTGCAGATCGA11110.20980196280972227No Hit
AGATAGCACTGTGGGACCAGCTCTTGGAAGGAAGGATCAAACTACAAAAA11070.20904660020734703No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11040.2084800782555656No Hit
GAGCACCGTGTCCTCTGTGTGGCTGGAGGTGGTGGAGAGAGCGACCAGGT10830.20451442459309563No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10770.20338138068953276No Hit
CGGCATGGACGAGCTGTACAAGTAATCTAGGGGGCCCGTTTAAACCCGGG10390.196205435966968No Hit
CTCCTTGCCAAAGCCCTTTTTGGCATAGGCTCGAAGTCTCAGCTCCTGGC10270.1939393481598423No Hit
CAGTCAGCCCGCGGAGGTGCAGGCAGGGTGGGCCCTCACTGGGGCAGCTG10210.19280630425627943No Hit
ACCCAAGGTGTTGTGTTTGAATCCCCTGAGGAAAGTGCTGATCAATCTCA10050.18978485384677846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.18770760669024655No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA9790.18487499693133944No Hit
GCTCTATTACTACCAAAGCCATCAGTCGGTGGTCATCTTTGGCTTGCAAC9770.18449731563015181No Hit
GTTGTTGACAGCGTCCCCGAATCCCGGCGTGTCCACGATGGTGAGCTTCA9710.18336427172658895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9710.18336427172658895No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC9490.17920977741352515No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9210.17392223919689848No Hit
GAGTGGGGCCTGGGATGAGAGTGGGGTATTTATCTATACCACAAGCAACC8990.16976774488383467No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8960.16920122293205325No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT8820.1665574538237399No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT8730.16485788796839562No Hit
GCTTCCTCCAAAGGGTCAGCCACACCTGAGGACACTAGACCAGACACAAG8520.16089223430592564No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8480.1601368717035504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8460.15975919040236278No Hit
GGGTAGAGCCGAGGTAGCAGCACAGGAAGCAATAATCCAGAACTCGTTAC8290.156548899342268No Hit
GTCCTGGCAGGAGTTGCAGAAAGCATCCACCTGTGGGAGGTCTCCACCGG8140.1537162895833609No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7690.14521846030663946No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG7680.14502961965604563No Hit
ACCAGGGAAATCACTTCCTTTAGGACTGAAAGCTAGGCGTCCTCTCCCAC7520.1420081692465447No Hit
AGGTAGGCCTTCAGGGTCCTAGAAGACAGGCCTTGAAATCCAGGCATCCC7210.13615410907813658No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT6930.13086657086150993No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACT6900.1303000489097285No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG6890.1301112082591347No Hit
CTAATCAGGAGGCCAGGCCACCCTGCCTCTACCCAACCAGGGCCCCGGGG6880.12992236760854087No Hit
CTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTC6770.12784512045200896No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAG6710.12671207654844613No Hit
GTGCCAGCCCAGAACACTGGTCTCGGGCCCGAGAAGACCTCCTTTTTCCA6520.12312410418716375No Hit
CTTTGGCTGGATGAAGCACTAGCCATCTCTTGCTTTGACAGGGCTTTTCT6350.11991381312706899No Hit
CTACAATCCTGGCCTTTGAGAGAGACACCCACTCAGCACAAGATTTCACA6320.11934729117528756No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG6300.11896960987409994No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC6200.11708120336816186No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA6200.11708120336816186No Hit
ACTGGAGGCTATATGAGTAAACAGCAAACCGTTTCTCATGAAAGCAGGAT6150.1161370001151928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6090.11500395621162993No Hit
ACGTGGTATGCAGCACCTCTCGGGCCTTGGGGGAGTTGATCCCCTCCAGC6000.11330439035628564No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5950.1123601871033166No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC5940.1121713464527228No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA5850.11047178059737851No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG5810.10971641799500328No Hit
CAGACCATGGGGTTTGCTTTTTATCAAGTCGTACTGTTTACATAAAGAAG5800.10952757734440947No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC5790.10933873669381566No Hit
GCATAGTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGA5780.10914989604322185No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGTGGGCGCCGGCGGCTTTGG5690.10745033018787756No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA5650.10669496758550231No Hit
GGACAAAGGTGGAGTTGGTCCGTACAGCAGTCTGGATGTCAGTCACCATC5640.1065061269349085No Hit
GGCACAGGGCAGGCACGTGCCTTGAAGTTTAGTGAAGGTCCCAACAGAGC5550.10480656107956424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5450.10291815457362614No Hit
GGATTACAGGCACCTGCCACCACGACCAGCTAATTTTTGTATTTTTAGTA5430.10254047327243852No Hit
TCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG5410.1021627919712509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)