FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724751
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC715909.877875297860921No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA179682.4791963032820927No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG86691.1961349484167665No Hit
CCAGCAAAGATCAGTCTCTGCTGATCAGGAGGAATTCCTTCCTTATCCTG73791.0181427828316207No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA60600.8361492429813825No Hit
GCACGGAGCCGCCTCCCTGGGGAGCAAGATGTTTGTCTGCGGGGGCTACG45940.6338728749598138No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG43700.6029657082225481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.5939971107318237No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC39000.538115849443464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.5110720785483567No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG36110.49824008521547397No Hit
TTCCTTCCTTCCTTCTTTTTTTAAACTCCTCAGCTTGCTATTGACTGGTA28970.3997234912404398No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA28410.3919966995561234No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC26570.36660866973622663No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC24690.340668726224593No Hit
CTGCAGTAGAGTCAACATTCTCCCAGGCAGCTGCTCCACCAAGCACATCA23190.3199719627844598No Hit
TCAGCAAAGATCAGTCTCTGCTGATCAGGAGGAATTCCTTCCTTATCCTG22300.307691883143314No Hit
GTCCAGGGCCTGGGTGAACTGGTTAATCGCAGGAGGCACTTTCAGCCGCT21310.29403201927282613No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG20960.2892027744701283No Hit
ATTTACAAATGTTGGAGTAATAAAGAAGGCAGAACAAAATGAGCTGGGCT20630.2846494865132991No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA19790.2730592989868244No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18440.25443221189070453No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18030.24877509655040145No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17830.246015528091717No Hit
GTTGATAAGGTCATGAAGGTTCAACAGATATTTATGGAGTGCCTAGTATG17430.24049639117434815No Hit
ACTTCCCCTGAAGGCTCCTCTCATAATTAAAGTTGCCCAAATTGCTGCTA16650.22973407418547886No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16470.22725046257266288No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA16330.22531876465158376No Hit
CTGTGCGGGAGGCCATAGTGATGGACAGGAAGGACCATCGATATGGCGAT16110.2222832393470309No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15830.2184198435048727No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15790.21786792981313582No Hit
ATCATGGGACGGGCAGGAGGTCTCATCATTGGGGGCCCAGGCATCATTGG15360.21193485762696426No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC14370.19827499375647634No Hit
GTCAGGTTGCTGGTCAAATTCCCTGAGTTGAATTATCAGCTTAAAATTAA13890.19165202945563373No Hit
AGCGAGGATTCAGCTTGATGGCATCTGTGAATAAGTCAATGGCTTTCTGG13320.1837872593483831No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC13130.1811656693126329No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT13120.18102769088969867No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC13020.17964790666035646No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12390.17095526601550048No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12370.17067930916963206No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.1690235680944214Clontech SMART CDS Primer II A (100% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT12070.1665399564816054No Hit
GCTCATTAATGATCTGAAAGAAGAAGATGGGGAAAAGGGGATTCCACCAC11850.16350443117705254No Hit
AGATCCTTGTTGCCGTCCCTTCGCCTCCTTCACCGCCGCAGACCCCTTCA11520.15895114322022322No Hit
CATCCAGAAAGAGTCCACCTTGCACCTGGTGCTCCGTCTCAGAGGTGGGA11320.15619157476153878No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11080.15288009261111749No Hit
GCAGCAAAGATCAGTCTCTGCTGATCAGGAGGAATTCCTTCCTTATCCTG10560.14570521461853794No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA10350.1428076677369193No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC10210.14087596981584022No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10120.1396341640094322No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC9900.13659863870487932No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC9850.1359087465902082No Hit
CCTGTGTGTCTGGGCCAGTCCCATGCCTGCCCCAAGCCCTGAGGCCCACA9780.13494289762966866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.13245928601685267No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9400.12969971755816825No Hit
GCGTGACCCAATGTGTGCCCCACAGCAGAGCCCACAGCCACGCCAGCTGC9250.12763004121415494No Hit
TCTCATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAAC9180.1266641922536154No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA9100.1255603648701416No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG9070.12514642960133895No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9060.12500845117840473No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9010.12431855906373361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9000.12418058064079938No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC8930.12321473168025983No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8930.12321473168025983No Hit
GCATGGAGCCGCCTCCCTGGGGAGCAAGATGTTTGTCTGCGGGGGCTACG8910.12293877483439139No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8700.12004122795277275No Hit
TCCCTGTCCCCTTCCTTCATGAAGGCATCTCCAGGCAAGGAAAACTGAAG8670.11962729268397009No Hit
GCCTCTGGTAGTTTCTGCATCTCCTTCAACATCATTCCAGCTTGCTCATA8590.11852346530049632No Hit
GAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA8450.11659176737941719No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT8320.11479804788127232No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8310.1146600694583381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8190.11300432838312745No Hit
GGCGCAGCCCGCTTCCTCAGGTGCCCTATCCCGCGCAAGAAGACCACGGC8010.11052071677031146No Hit
GCCTAGGAGGCATAGTGAGATCTTGTTTCTGCTAAAAATGAAAAAAATGA7970.10996880307857457No Hit
CTCCTGTCCCCACTGGTTTGATATAAATGATTCTAAAGTCCAGCCAATCA7910.10914093254096925No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7740.10679529935108747No Hit
GGGCTCATGGTCTGCCCTCGAGGCCCCTTTAACAAACGCGCACTCTAGCC7590.10472562300707416No Hit
CCTTAAGACCATGGCTACTACTGGTGTCCTGGTCCTTTCCTTATTTCCTG7490.10334583877773194No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC7390.10196605454838971No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)