FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595770
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC6088210.219044262047435No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA169172.8395186061735234No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG72531.2174161169578863No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA59200.993672054651963No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC39010.6547828860130587No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG39000.654615036003827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.5955318327542508No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30870.5181529784984138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29780.499857327492153No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26380.44278832435335785No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC22200.37262702049448615No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC20040.336371418500428No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17170.28819846585091563No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA16880.28333081558319484No Hit
CTCTACGAGGAGGCCGAGGAGGCCAATGACCTTTCCCAGCAGGTGGCCAA16860.28299511556473134No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15990.2683921647615691No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA15400.2584890142168958No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14910.25026436376454003No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT14250.23918626315524447No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13870.23280796280443794No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13690.22978666263826644No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG13490.22642966245363144No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13480.2262618124443997No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12990.21803716199204393No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC12970.2177014619735804No Hit
AGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCGGAC12230.2052805612904309No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11070.18580996021954782No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT10680.17926380985950954No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10480.17590680967487451No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10440.17523540963794754No Hit
GGCACTGAGTGCTTCTTGGGATGCCAAGGGAAGGCCAAAGCCACTTAGAG10430.17506755962871579No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.16600365913020124Clontech SMART CDS Primer II A (100% over 21bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9790.16432515903788375No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC9670.16231095892710273No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9070.1522399583731977No Hit
GCCTTGCTGAAGCCCACTTTGCCACTGGGCAGTCGCATAAGCTCGCTCTG9070.1522399583731977No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8990.1508971582993437No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8890.1492186582070262No Hit
GTCAAAGAACCTCTGGGTCCATGGGTAGACAACCAGGAGCCTTCCCAGGG8880.14905080819779445No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA8820.14804370814240395No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8800.14770800812394044No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8720.14636520805008643No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8610.1445188579485372No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG8610.1445188579485372No Hit
AGACAGCCCCATCACCGTGACCATCAACATGAAGCCCCTTCAGCTATACC8590.1441831579300737No Hit
GTACATGGGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAA8530.14317605787468318No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8220.13797270758849892No Hit
ACCTTGACCAGGCTGATCTTGAACTCCTGACCTCGTGATCCACTCACCTC8130.13646205750541318No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA7960.1336086073484734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7840.1315944072376924No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7650.12840525706228914No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTAT7610.12773385702536214No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA7570.12706245698843516No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7560.12689460697920338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7440.12488040686842238No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7340.12320190677610487No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7340.12320190677610487No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC7330.12303405676687311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.12303405676687311No Hit
TCTCACCACTCACATTTTATTGAGAACAGTGGGCAGGGGGGCTGGGAGAG7200.12085200664686037No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7100.11917350655454287No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6310.10591335582523456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6290.10557765580677107No Hit
GAGCCACCGCGCCCGGCCCCCCACTCCCATTTCTTAGCAAACTTGAAGCC6080.10205280561290432No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6000.10071000553905031No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)