FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565981
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT458788.105925817297754No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA127632.25502269510814No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG58631.0359004984266256No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA43220.7636298745010874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.6351803329087019No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG32030.5659200573870854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29070.5136214819932118No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC27060.4781079223507503No Hit
GTCCTCTCAGCATATGAGTGGCCTCCTTCTGTCTGGAGCACCTCTGTGAC24260.4286362969781671No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG23980.4236891344409088No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC22480.3971864779913107No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21960.3879988904221166No Hit
GGTTTATTGGCGCATTACGCGGTAAGTGGGGCTGGGGAACCTCCAGGACG15350.2712105176675542No Hit
GTGGAGTACGCGCAGGAGGCCGTCAAGAAGGGCTCGACCGCGGTTGGTGT15120.26714677701194917No Hit
GTGCAGGGGGACCTGGAACATGGATGGGAGTGGACAGGCCTTTCTCCTTA14750.260609455087715No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14230.2514218675185209No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC14210.2510684987658596No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT13910.24576796747594No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13880.24523791434694803No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13710.24223427994932695No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13600.2402907518096897No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12650.2235057360582776No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC12430.21961867977900318No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC12400.21908862665001122No Hit
GGATCACTCTGCCAAGCGGCAGGAAGAATTAAGGAAACGACAAGGAGACG11860.2095476703281559No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11030.19488286709271158No Hit
CTGGAAGAAGATGAAGAGGAACTTTTTAAAATGCGGGCAAAACTGTTCCG10850.19170254831875982No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10300.18198490762057384No Hit
GTTGATGAGCAGCTCCTGCTGCAGAAACTGCTCCAGGATGTACTTGGGGG10260.1812781701152512No Hit
GTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCCCCGTC9920.17527090132000897No Hit
GTCATGTTCTGAAGTACTGAGGGTTAGGGCTTCAACTTACGAATTTGGTG9850.17403411068569438No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9780.17279732005137982No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9630.17014705440642No Hit
GTGGTGCTCCTTGACGTGCTCCACCAGCTTCTGGCTGAAGATCTGCCGTC9220.16290299497686317No Hit
ACCACGCCTGGTGCGCACCACCACGCCTGGCTAATTTTTGTATTTCTTGT9160.16184288871887925No Hit
ACTGTGAGGAGGCAGGCATCCCACTGGTGGCTATCATCGGCGAGCAGGAA9000.1590159386975888No Hit
TTCTTACTACATTGGGGAAGTGTTTGGGTGGCCAGCCATAAAGAGAGATT8960.15830920119226619No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.15760246368694356Clontech SMART CDS Primer II A (100% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT8620.15230193239702394No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC8560.15124182613904No Hit
GTCGTGTACCTGTCGGCGATGGAAGAGCAGAAGCACACCGTAGCCCAGGC8420.14876824487041085No Hit
ACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACT8420.14876824487041085No Hit
GAGTTGGCCATGCTGACAGCTCTGTGCCCTCGGCTGATGGCAGTTGGTAG8330.14717808548343495No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8310.14682471673077366No Hit
TCTCAGGATCCACATGCAGCTTGTCACAGTGCAGTTCACTCAGCTGGGCA8150.1439977667094832No Hit
CTCGTGATCTCGCGGCAGTTGCTGACTGATTTTTGCACACATCTTCCTAA7890.13940397292488618No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7870.13905060417222487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7790.13763712916157964No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG7710.1362236541509344No Hit
GCATTTTGCTAGTGCAATTATTGTCCCTTTCAGACAGCAGAACATGAGCA7670.13551691664561177No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7520.13286665100065198No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7490.13233659787166002No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG7470.1319832291189987No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC7460.13180654474266804No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7390.13056975410835345No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC7360.1300397009793615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7250.1280961728397243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.1273894353344017No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7180.12685938220540974No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC6940.12261895717347401No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC6680.118025163388877No Hit
GGATTATGGGGTCTTAAAGGCTGATGAAGGCATCTCGTTCAGGGGCCTTT6590.11643500400190113No Hit
GTGAACTAATTTAGAGCATCCTCCAGAGCTGAAGCAGAACATTCCAGAAC6580.11625831962557047No Hit
GGTATATGGTTAGTGTGTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATG6550.11572826649657851No Hit
GACACACAGCAGCTGCAACACGGAGCTCCAGGCCGCATGGAATTTGTACG6370.11254794772262673No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6180.10919094457234431No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6160.108837575819683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6120.10813083831436036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.10636399455105383No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6010.10618731017472319No Hit
GGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAAT5940.1049505195404086No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA5930.10477383516407796No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAT5820.10283030702444075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)