FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460180
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC5985513.006866878178105No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA137322.9840497196749096No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG77821.6910774044938939No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48261.0487200660611065No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG40400.8779173366943369No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC31050.6747359728801773No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30180.6558303272632449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.5530444608631405No Hit
AGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCT22820.495892911469425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.4615585205789039No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC19900.4324394802033987No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC17590.382241731496371No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16260.35333999739232475No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15650.3400843148333261No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC15250.3313920639749663No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14380.3124864183580338No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13750.29879612325611715No Hit
GTATAAATGGGAATGTAGCCCCGACTTCCCAAGGTGCGAAGGGCATACTG13360.29032117866921636No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12900.28032509018210267No Hit
ACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC12690.2757616584814638No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC12530.27228475813811986No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12010.2609848320222522No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA11960.25989830066495717No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10660.23164848537528793No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT10170.2210004780737972No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC10110.21969664044504325No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG9750.21187361467251944No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA9380.20383328262853664No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9350.2031813638141597No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9140.1986179321135208No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC8810.19144682515537398No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8540.18557955582598112No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8380.1821026554826372No Hit
ATTCCTGAGGACGCCATCCCTGAGGAGAGTGGCGATGAGGACGAAGACGA8250.1792776739536703No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC8150.17710461123908036No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8060.1751488547959494No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8040.17471424225303142No Hit
GGCACAGTGGCTCACGCCTGTAATCCTAGCACTTTGTGAGGCCAGGGCGG8020.17427962971011343No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT7940.17254117953844147No Hit
GGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTG7920.17210656699552349No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC7860.17080272936676952No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7800.16949889173801555No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT7750.16841236038072058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7700.1673258290234256No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7590.1649354600373767No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7450.16189317223695077No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG7180.1560259029075579No Hit
GTACATGGGACCAAGGCCAGTCCTGAGCAGGCCCAACTCCAGTGCAGCTG7110.15450475900734495No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6970.15146247120691902Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG6940.15081055239254204No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC6840.1486374896779521No Hit
CATCATCTCAGGCGGGCTGGCCGGGGCCCTCGCCGCGGCCGCCACGACCC6260.13603372593333043No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6180.13429527576165848No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC6080.13212221304706853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5420.11777999913077492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.1175626928593159No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG5380.11691077404493892No Hit
GTTTAAAGCAGCTGCCAGCTCGATATCTCATTAGCGTGACTAGGATTTTA5370.11669346777347994No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5030.10930505454387414No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC5030.10930505454387414No Hit
GAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAG4890.10626276674344823No Hit
GAGTACATGGGGGAAGAAAAAAGGCTGAAAGAAAAGGGAGGGGCTTAGGG4750.10322047894302229No Hit
ATGTGGGCCAGACTGTCGACGATCCCTACGCCACCTTTGTCAAGATGCTG4750.10322047894302229No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG4730.10278586640010431No Hit
GGAGGGGTACTTTCCTGTGGCAATGGCGTTAAAAAGATCCCGGATGCCAT4660.10126472249989134No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG4640.10083010995697336No Hit
TTGCTGGGCCTCAGCCTGCTCTTCCTGGTGGCCCATCTCGCCCTCCAGAT4640.10083010995697336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)