FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541333
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT148792.7485854363210818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42470.7845448180694693No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA39640.7322664607552098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.6408255177497031No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA29180.5390397407880178No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC23340.4311579009592986No Hit
GAGCAGCCCCTGGATGAGGAGCTGAAAGACGCCTTTCAGAACGCCTATTT22190.4099140455135748No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20430.37740171022272795No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC19230.3552342088880596No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16410.3031405807515891No Hit
GACTGAGACTCAGCCTCCTTCATCCCAATCCAGGCTGCAGCTCCAGATAG16010.29575141364003305No Hit
AAATCAAACCTTGATAATAAAGTATGAGGCAGCTGGATGTTTTCAATGGT15820.29224155926204387No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15500.28633022557279897No Hit
CTGACAGAAGTGAGAAAAGGAGGCAGATGAGAAAGTGGGATGGGGAGCAG14800.27339918312757583No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT14650.27062824546074227No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC14560.26896568286064215No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14230.26286961999360836No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT14070.2599139531489859No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13380.24716763988155166No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC13350.24661345234818494No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11960.2209360966355275No Hit
CAACAAAACGTTGGAATAGGGTTCGCGAAAGTATGCTACATGAGTATGAG11930.2203819091021608No Hit
CGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATC11820.21834988814648285No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC11280.2083745125458821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.1996922411898037Clontech SMART CDS Primer II A (100% over 21bp)
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10610.19599765763402563No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10410.19230307407824757No Hit
GTATATTTATGTAGGTGTGAGTGATTGCCTGGTGCTTGCTTGTGCCAAGG10010.1849139069666915No Hit
GATCTTACGGGCCGGCTGGTTGAACCACGTGGCCACGCGCCGCTGCCAGT9770.18048040669975782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.1780789273885021No Hit
GCTATAACCAGCTATGAGAAATCAGATGGTGTTTACACGGGCCTGAGCAC9480.17512326054387964No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA9390.17346069794377952No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9020.16662571836559012No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG8980.1658868016544345No Hit
GAAGTTTACCTGGTCCTCTCCCTCTGGAAAGAAGGCATTGATGATCCGGT8860.1636700515209677No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC8510.1572045302983561No Hit
ATGTTATCAGAATCCCAGGGGGTGAGACCTAGGCAGGAGTGTTTTTCTAA8340.15406413427594476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8320.15369467592036695No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8310.15350994674257804No Hit
CCGCCAGCTCCTGCTTCACTTCGGCACAGTCATTTATTTTCTCTTCCAAC8130.1501848215423778No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8100.14963063400901108No Hit
AGATGGGCAGATGATGTAGATCGCCGGGTGCAATTTGGCATTGAAAGTGG8070.1490764464756444No Hit
TACCGGAGCAGCTGGGACCACAGGCACGAGCCACCACACCCGGCTAATTT7940.14667496716438863No Hit
AGACAGGCCGAACCCAACTCTTCACAGGGCCGAATTCTTTGCCCGCAGCC7680.14187200854187718No Hit
GTTGTTTTACCACTCCCTTGCAATCCAACAAACATAATCACATTTTGTTT7570.13983998758619925No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG7460.1378079666305213No Hit
TCGCCAGCTCCTGCTTCACTTCGGCACAGTCATTTATTTTCTCTTCCAAC7450.13762323745273244No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG7360.1359606748526323No Hit
GAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGAT7330.13540648731926558No Hit
GGCAGGAGGATCGCTTGATGCCAGGAGTTCGAGACCAGCCTGGGCAACAC7310.1350370289636878No Hit
ATCTTACTGTATGACAAATTGCCCAAACTGACATAACACAGTATTTTAGA7250.13392865389695438No Hit
CTAATAACTTTGGCTGTTTATTCTGGTATTTTCATCTGTTTCTAGATATG7240.13374392471916546No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT7210.13318973718579877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7160.13226609129685424No Hit
GTAAAGGGCCCGAACAGCTTCACCACGGCGGTCATGTACTTCTGTCCCCA7140.13189663294127643No Hit
TGCTTCCGGATGACTGAGTGGAGGAGAGGGAAGCAGAACCGCGCCGCGAT7040.1300493411633874No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA7010.1294951536300207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6990.1291256952744429No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6990.1291256952744429No Hit
AGCATCTAGTGAGTAGAGGCCAGGGGGTTTGCTAAACATCTACAATGCAC6910.12764786185213167No Hit
GGGCATGGACAATGCCGCGCTCGCGACCGTCACCGGGATCGAGAACGGCC6790.12543111171866486No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6640.1226601740518313No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6510.12025869474057557No Hit
CTTCCAGCATGGGACGGCTGGGAGGATCCACAGACTGGCGTCTGCCGGCG6390.11804194460710875No Hit
TCATTTTAAGCTGCAGCAAATCATCGAGCATCATCACTGCATCCACCACC6340.11711829871816425No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC6320.11674884036258643No Hit
GGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTT6320.11674884036258643No Hit
CAATGTAGCAGCACAAGGGAAGTACATTGCTATAGTTAGTACAACTGTGG6270.11582519447364192No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT6040.11157642338449716No Hit
ACATCCAGGGGGCTCTGCACAATTACCGCTCGGGCCGCGGGGAGCGCAGG6030.11139169420670825No Hit
CTTCTTCATCGTCCTCTTCAGCCTCCTCGGTGTCATCCTCATCTTCCTCA6000.11083750667334154No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCG5990.11065277749555263No Hit
GCTCGGAGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCG5940.10972913160660813No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT5840.1078818398287191No Hit
GAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTAT5800.10714292311756349No Hit
TAACAATGTCAAAATGTCCTTCCAGTTGAGAGACATCTGTCTCATTATCC5570.10289415202841873No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT5540.10233996449505202No Hit
GTTTGTGTATTCGGCTATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATT5540.10233996449505202No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG5460.10086213107274081No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT5430.10030794353937411No Hit
CATGTGAGCAGGCAGATCTTGCCTTCTCTGCCCTGCCAGTGTGCATGTGT5420.1001232143615852No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA5420.1001232143615852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)