FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501341
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA242574.838423348579111No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA73641.468860516095831No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA35470.7075024783530571No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33320.6646174958760604No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31240.6231287686425008No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA26380.5261887617410106No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA26160.5218005309759226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25130.5012556323939196No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC18670.3724012199281527No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18410.36721512902395775No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC17760.3542499017634704No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC15200.3031868528606278No Hit
TAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG13600.2712724472963512No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12830.2559136396185431No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12820.25571417458376633No Hit
GTTTATAACGTGTGTAGTGTATTTGGAGTGAGGGTGCATGCTCCCTTTTC12510.24953075850568776No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12440.24813450326225064No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12120.24175162214939533No Hit
GAAGGAGCCCGTGCCAGCTGATACTGGAGTAGGAGTGGGTGAAGACCCTC11840.23616660117564692No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11270.22479709419337338No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT11260.22459762915859666No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10550.21043561168944888No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC9520.18989071310744585No Hit
ATACGGCGCTATCCGCGACATCGACCTCAAGAATCGCCGCGGGGGACCGC9500.18949178303789238No Hit
GCATCATTCCCTAGGAGGTCAGTATGGTGTTCAGGGATTTCCTACCATTA9410.18769659772490183No Hit
GTTTCACCGTCACCAGATGCACCACAGTCACCAGGGGGCATGCCACACTT9390.18729766765534836No Hit
GTAGCCTCAAACTCCTGGGCTCAAGGAACTCTCCAACCTCAGCCTCCTGA9320.18590141241191124No Hit
TCCATCTGCAGTTTCTGCACCTCAGCACGCACGTTGGTGCCCCCGATACA9300.1855024823423578No Hit
GTGTCAATACTTTATTTTGGTGTAAAGACAGGAAGCTGGAAAATACACTG9260.1847046222032509No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9060.1807153215077163No Hit
GTTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAACTTGATG8900.17752388095128863No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT8800.17552923060352135No Hit
CCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA8730.17413297536008426No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA8420.16794955928200567No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGA8370.16695223410812202No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA8240.16435918865602453No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8180.16316239844736416No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA7960.15877416768227615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.15638058726495538Clontech SMART CDS Primer II A (100% over 21bp)
GCAGCGTCAGATGTGTATAAGAGACAGCCATTATTCCTAGCTGCGGTATC7510.14979824111732334No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7480.14919984601299316No Hit
GTGATGATGAGGAGTGCTGATACAGGAGCTTAGATGATTCTATCCAGGTG7390.14740466070000258No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT7240.14441268517835165No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7220.1440137551087982No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA7120.1420191047610309No Hit
GAGTCTTGCTCTTTCACCCAGGCCGGACTGCAGTAGCACTATCTCGGCTC7060.14082231455237054No Hit
GTACTAAGGGTACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCT7010.1398249893784869No Hit
GAGTACATGGGCAGGCGGATCACGAGGTCAGTAGATCAAGACCATCCTGG7000.13962552434371017No Hit
ACGCAGAGTACATGGGGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCAT6930.13822926910027308No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT6840.1364340837872825No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC6810.13583568868295232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6800.1356362236481756No Hit
GTTCCTACCAGTTCCTCTTCGCCTTGTTCTGAGGGAGGTTTGGAAGTTTC6790.13543675861339885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.13443943343951523No Hit
GTGCAGCAGGGACAGGTGCGTCACTGAGGTGTTTCCTGCCAGCCCCTCCA6620.1320458530221945No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC6620.1320458530221945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6590.1314474579178643No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC6540.13045013274398065No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT6500.12965227260487372No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA6460.12885441246576682No Hit
GAAATGAGTTCAAAAGCTGCCGCTCAGCTTTGATGGAACAACGCTTATTT6380.12725869218755298No Hit
CTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAACACAATGGGG6360.1268597621179995No Hit
GTACATGGGAGAGTTCTTCCTTCCCCATCTGGGCCTTCATAAAATGCAGG6330.12626136701366933No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA6310.12586243694411586No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT6150.12267099638768822No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC5960.11888116072693038No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG5770.11509132506617252No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG5740.11449292996184235No Hit
AGGTTGGTGTCTTCAAAAAGGCCAACCAGATAGGCCTCACTTGCCTCCTG5660.11289720968362851No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT5620.1120993495445216No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC5510.10990523416197757No Hit
TTCCCCAGTGGCAGGCTGCCCCTGCAACTGGTCCTGCTATTAGAGAGAGG5480.10930683905764739No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC5470.10910737402287066No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT5450.1087084439533172No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5440.10850897891854047No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5330.10631486353599646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5300.10571646843166628No Hit
CTTCGGCATACGGGCAAAAAGAGCCAAGGAAGCCGCAGAACAGGATGTTG5240.1045196782230059No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG5230.10432021318822918No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC5160.10292395794479206No Hit
GTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATT5120.10212609780568516No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT5100.10172716773613169No Hit
ACGCAGAGTACATGGGACGGACAGGATTGACAGATTGATAGCTCTTTCTC5070.1011287726318015No Hit
ATGTGTTATCTCATTTAATTCTTACAAGAACCTTTTAAGATAGGTATTCT5040.10053037752747132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)