FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525761
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA314735.986180032372124No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA84111.5997763242233638No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38320.7288482789708631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.6156789872204291No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA28820.5481578131508423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28000.5325613729432194No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24280.46180679053790596No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC23210.44145533807186155No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG19950.37944997822204385No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC18570.3532023105555566No Hit
CTCTCCAGGTTTCCAGATGCGAAGCCCTGATAGGCTGAGTCAAAGAAGGG16150.3071737918940355No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15900.30241877963561387No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15390.2927185546284338No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14260.2712258992203682No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13560.2579118648967877No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT12890.2451684320442178No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA12630.24022321929545934No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12330.2345172045853534No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG11140.21188334623526658No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10860.20655773250583437No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10470.19913991338269668No Hit
GTCCACCACCACGCCGGCTAATTTTTGTATTTTTAGTAGAAACGGGGTTT10230.19457510161461197No Hit
GTACAAACACAAGTTGGTGGACTTTGTGATCCACTTCATGGAGGAGATTG10120.19248289622090645No Hit
GTACTGCACAAAGTGGGTAGTTGTAATCCAGTGTGAACACGTCATCTGCC9950.18924948788517978No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.18620628003978995Clontech SMART CDS Primer II A (100% over 21bp)
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC8530.16224101825734505No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8320.15824680796027094No Hit
CTTTGGCGGTAGGATTCATCATCGGCTTCTGGGCGCTGTTCCTCGGAGTA7920.15063878834679637No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7820.14873678344342772No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT7790.14816618197241713No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7620.14493277363669044No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG7560.14379157069466925No Hit
ACGCAGAGTACATGGGAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG7500.14265036775264806No Hit
GAGTACATGGGAACATTAGGTAGAATTCTTAAATCTATTTAAAAGGAAAA7390.14055816235894256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7300.13884635794591077No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7170.13637375157153156No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG6960.1323795412744574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.1323795412744574No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6890.13104813784209937No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6810.12952653391940444No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6710.1276245290160358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6510.12382051920929853No Hit
GCCTTCAGGGATGAAGCTACTCCCTGAGGTCCCAGGGACGGGAGCTGGCC6440.12248911577694048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6340.12058711087357184No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6340.12058711087357184No Hit
TCTCTGGAGACCACCATCTTTACTTTATTCCATCTTTCAGGATTATCCAG6340.12058711087357184No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT6320.12020670989289811No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC6190.11773410351851887No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC5990.11393009371178159No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5890.11202808880841295No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5890.11202808880841295No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC5820.1106966853760549No Hit
CTGCATAACTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGCCTT5590.10632207409830702No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5590.10632207409830702No Hit
CTTCCACCTTCTCCATGAGAGGACGAAAGACTAAGTGGGTGTCAGTGCCT5470.10403966821426465No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5450.10365926723359092No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA5430.10327886625291718No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC5420.10308866576258034No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC5270.10023565840752736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)