FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638203
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC472727.4070476008417385No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA133292.088520423752317No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG54950.8610113083141256No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA46020.7210871775908292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.5723884093305734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.4598850209102746No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG28380.44468609517661306No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC26420.41397486379725573No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG24450.38310694246188126No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24010.37621258439712757No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC20380.3193341303629096No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA18310.28689930946736386No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC15580.24412295147468754No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15170.23769866327798522No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG14690.23017754538916302No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT13540.21215820044719313No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12400.1942955454612404No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12070.18912477691267512No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11980.18771456730852096No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC11560.18113358915580152No Hit
GAGTACATGGGATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCC11330.17752972016740756No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11260.17643289047528765No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10730.1681283228063798No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT10650.16687480315824274No Hit
TGAATAGAACAGCAATGAGAGCCAGTCAGAAGGACTTTGAAAATTCAATA10410.16311424421383167No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10310.16154734465366036No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA10250.1606072049175576No Hit
GGCTGGTAGACAGGGTACACGTCCCCCACCCAGCACATGAATATCATGAA9900.15512305645695806No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT9780.1532427769847525No Hit
GAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGA9740.152616017160684No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9590.15026566782042705No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9530.14932552808432428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9240.1447815193598275No Hit
TCCCCTGCTACTCAAGGCCAAGGTAGGAAACAGTCTTTCCTGTTTTCTTC9150.14337130975567336No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9120.14290123988762196No Hit
CCTAAAACCCGGTTCACACCCATGCCATCAACCCCCAGAGTTCAGCAGGC8890.13929737089922797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.13428329230667985Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8400.13161956305438866No Hit
GTGCAAGAAGAAACACACGCTGCTGTGCCCCGACTTTGCCCGCAGGGGGG8380.1313061831423544No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC8340.13067942331828586No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG8060.12629210454980624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.12472520498963495No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7910.12394175520954931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7560.11845760674894978No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG7510.11767415696886413No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT7180.11250338842029887No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7120.11156324868419609No Hit
GATGTGTGCTAAGTTCAGCCACACATCACTAATATCTGCTGTTGCTTCTC6950.10889951943190489No Hit
TTCTAACTCCGCCTGGAAGCGTGGCAGGCTCTCCTTATGTCGCTCAGGCT6900.10811606965181926No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6740.10560903035554518No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6630.10388544083935675No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC6520.10216185132316832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)