FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915667
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA716417.823914152197251No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA219712.3994530762820983No Hit
ACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT117531.2835452189496837No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG103001.1248630779530113No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA82850.9048049127029804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61040.6666178861966195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56520.6172549627757689No Hit
TGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGCGTCTTCACCACCATGG56350.6153983926471086No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC47460.5183106959189312No Hit
GATCAGTCTCATCTGAGAGTAACTTTGTACCCATCACTGATTCCTTCTGA46330.5059699650637186No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG44570.48674900373170593No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA42700.46632673231644256No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG36540.3990533676543984No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA34960.38179818645861435No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC30600.3341826231588558No Hit
TCGCCGTGGTGGCCGAAAACGCCCAGTTCCTAAGGGTGCAACTTACGGCA28590.31223141163763685No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC27510.30043673082026545No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA24730.27007634871629094No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA24570.2683289885951989No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA22330.24386594689991015No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC22190.24233700679395456No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA22040.24069885668043076No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT21730.2373133464458149No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.23228968609767525Clontech SMART CDS Primer II A (100% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC21030.22966864591603717No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC20360.22235157540896416No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT20310.2218055253711229No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC20150.22005816525003086No Hit
ACTTAAGGTCATCAAAGCCCACTACACGTCTTCCATCAAAATGCCTTTTG19880.217109495045688No Hit
TAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG18360.20050957389531346No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA18300.19985431384990396No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA18070.19734248367583412No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA17860.1950490735169008No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA17330.18926094311578337No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC17270.18860568307037384No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16520.1804149325027548No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16280.17779389232111675No Hit
CAACAAATGGAGCCTGGAGCTAGAGGACCAGGAGCGGCACTTCCTCCAGC15970.1744083820865009No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15730.1717873419048628No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA15700.17145971188215803No Hit
GGGGTGCACTGAGAGATCAGGGGGATGATGGTCTTCCCAGCATGGCCACC14920.16294133129183427No Hit
CGTTATGCTAAATAAATGAAGCCAGTCACAAAAGGCACGTATTGTATGAT14700.1605387111253327No Hit
TCTGCTTTTCTTACTCCCCACCTCTGCTGATAATTCCTAGTTCATTGGTT14530.15868214099667238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14390.15715320089071683No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA13800.15070981044418985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13170.1438295799673899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.1412085397857518No Hit
CATCTTGCTCCACAGGCTAGTGACGGCAGCCTTCTCCTCAGCAGTAAAAT12410.13552961939220262No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC12040.13148884911217726No Hit
ACGCAGAGTACATGGGTGGAAAGATGGGCTATAAACACAAGGCTATTTTG11770.1285401789078344No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA11660.12733886882458362No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA11520.12580992871862806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10710.11696391810559952No Hit
CTTGAGGTGTCTGATGATGTGCCTGAATCACTATCACTGTTGCTAGAACT10570.11543497799964397No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10330.11281393781800589No Hit
TCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCA10310.11259551780286939No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG10180.11117578770448208No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC10160.11095736768934558No Hit
GCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTGGCTGGG9760.10658896738661545No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC9730.10626133736391069No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9590.10473239725795513No Hit
GCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA9430.10298503713686306No Hit
GCAATCGCAGGCACTGCTGTGGTCGCCGGTCGGCCGAGGGGCCGCGATAC9370.10232977709145356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)