FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008277
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC610656.056371413807912No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA147941.4672555260112052No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG71520.7093288848203421No Hit
GATAAAAACTTTGTGGTTAAAGGTTTCTCCTTAGGACTAGCGACAAAGTT67910.6735252316575703No Hit
CAGTGGGCACCTTCTTTCCATGCTGTTCATCAAATTCTGAGTGCAAATCT63320.6280020272206943No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA61950.6144144912558751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58570.5808919572696789No Hit
GTATAACATTCACCTGGCTGTCAACTTCTTGGTGTCATTGCTCAAGAAAA55100.5464768114317792No Hit
CTGATAGGTCTCAGGCTACAGGAACGGGGGACACGGGCAGCTCAGGTCAG52190.5176156948933676No Hit
CAGCTGCATGATCCTGCAGGAGCCGGCCAGGCCCCCTGCCTCTCTCTGGT51600.5117641283099783No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48990.4858783846105783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.48478741456960733No Hit
TTCCCAAGCAGCAGCGGTGTGGAGAAGTAGGTACATTCCGTTTCCTGTGA40580.40246876602362247No Hit
CAGTGTCATAGATACCGAAGTAGGCGGCTCGGTAGATGATAATACCCTGC36230.3593258598579557No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG35490.35198660685506067No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA34500.3421678764863227No Hit
GTCCTGGTCCGCGCCAGAGCCCAGCGCGCCTCGTCGCCATGCCTCGGAAA34370.3408785482560844No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC32470.3220345202756782No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31320.31062892439280076No Hit
CGGTTCCCAACCCCAAGGGGGAATTAGGGGATTAGATTAAGAGTAGGTAA30660.3040831041469755No Hit
CTGTATCCCGGTACTGAAGAGGTACGCCAAAGAAGGGAGAGAATTTGATT30150.29902497032065595No Hit
ACCCAGGCTGGAGTGCAGTGGCGCAATCTTGGCTCACTGCAACCTCCACC29040.28801609081631335No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC28980.2874210162485111No Hit
GGCAAAGACCGGTCAAGTTTTTACGTGAATGGGCTGACACTTGGGGGCCA28400.2816686287597555No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG25020.24814609477355926No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC24870.2466584083540535No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC22520.22335132111513006No Hit
AGCCTGCAGTGCTACAACTGTCCTAACCCAACTGCTGACTGCAAAACAGC21820.2164087844907699No Hit
GCCGAGGACTTTGATTGCACATTGTTGTTTTTTTAATAGTCATTCCAAAT21480.21303669527322355No Hit
GGACCCGCGCAGCCCAGACCGTTCCCGGGCCGGGCAGCCGGCCACCATGG20480.20311878580985188No Hit
CCCTTTGGGGCCAGTACATTGTATGGGTCCAGTCCCTTCTTTGCAGCCAG20200.2003417711601078No Hit
GAAGATGAGAGAGGGTCCTGCAAAGAATATGGTCAAGCAGAAAGCCTTGC19880.19716804013182884No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19760.19597789099622423No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA19350.19191154811624186No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG19310.191514831737707No Hit
CAAGGACATTTTCCAACGAGGCAAAATCTGCTTCATCCCTCGGTACCGGT19120.1896304289396664No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA18700.18546490696505027No Hit
AGGTACAACTCTTTTGTCGTCCATATCACACTTGTTTCCTAGTAACATTC18380.18229117593677135No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18290.18139856408506788No Hit
TCTTTGAAGAGATGGAAGTCACTGTGGCCTGTCAGGTCCCCAGGACGTAC17440.17296834104120198No Hit
CCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATC17340.17197655009486482No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC17150.17009214729682418No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA16670.16533155075440578No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16480.16344714795636517No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16060.15928162598174905No Hit
CTCCTCTTCATCTTCTGTCTCTGATTCATCCTCACTTCGCTTCTTACGTT16040.15908326779248164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.1583890141300456Clontech SMART CDS Primer II A (100% over 21bp)
TCTGGAACCCGTGTGGACCACCAGACAGGCCCCATTGTGTGGGGGGAGCC15660.15531446219640038No Hit
CTGTTAATGCGCAACTCCACCACGTTTCCATAACTTTGAAAGAAATCTTT15650.1552152831017667No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15630.15501692491249924No Hit
ACTCTGCACAGCAGCCCAGCAGGGATGTCCTCTCTAGAGATGGTTTATCA15420.15293416392519119No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14730.14609080639546473No Hit
CACGCAGGTCGCGCGTGAAAAGGCGCTCAAGAAATTCCGTCATGGCGAGG14270.14152856804231376No Hit
GCCGCAGCTGTCCTCCAAAATCGCCAAGGCCTCGATCTGCTTACTGCTGA14180.14063595619061034No Hit
AAGCAGTACAGGCGCTTGGCAGGGTTTTCAGACTTCTTGTTAGCATTACA13610.13498274779648847No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG13480.13369341956625014No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC13100.1299246139701689No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA13030.1292303603077329No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA12770.12665170384725627No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12610.1250648383331168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12580.12476730104921563No Hit
GTGCAAATCAGTCCATTTCACTTTCACTGTCTGAGATAGGGTCTCTAAGA12420.12318043553507618No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12400.12298207734580875No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12330.12228782368337272No Hit
GAGCACGCGTGTGTGCGGACGCAGTTGCGTGAGGGGTTTGTACTATCCTC12250.12149439092630299No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12110.12010588360143096No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA11750.11653543619461716No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11350.11256827240926849No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11250.11157648146293132No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT11250.11157648146293132No Hit
GTCGTCCTCAACCACAGTCACTGAGCGCACCAGCTTGCGCATGGCCACTT11240.1114773023682976No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC10920.10830357134001865No Hit
GCTCCAAGGTGGTGGCCGCCGTGGGCGACGCGGTGAAGAGCATCGACGAC10860.10770849677221637No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10860.10770849677221637No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC10770.10681588492051292No Hit
GGCCTGGCTTCCTGGCATCACTCGTCATCAAAGTTCTGACTCAGGAGGAA10650.10562573578490832No Hit
CTCCTGCCGCTGCTTCAACGGCTCTTGGGCCAGCCAAGCCTGGACTCCCT10600.10512984031173973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10530.1044355866493037No Hit
CAGGAGACCGCGTATTCTTAGATTCAGCCGCCGAAGCCATACAGAGTGCG10510.10423722846003627No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT10370.10284872113516423No Hit
GGGTAATTTTTTCATTAAAATTCTTCTTCTGCCTGTTTGCTGCGGAACTC10280.1019561092834608No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT10230.10146021381029222No Hit
TCTTGGGAGCGGCGCCGCCTCTCGCCGGGTCACCGGCTGCACTCGCCCGC10190.10106349743175735No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT10120.10036924376932133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)