FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904242
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT422154.668551117952938No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA114751.26901869189885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66690.7375238044682729No Hit
CAAAAAATGTGGAGTATTGAAAATATTGCCTTCGGTTCTGGTGGAGGTTT62240.6883113148913675No Hit
TCACAGGACAGCTGCCTATAAAACTAGACTTCTGACGCTGGGCTCCAGCT59090.6534755076627717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54740.605368916728044No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG50460.5580364548428408No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA44550.49267784509014184No Hit
GTGCTGGACTTGAGCTCTGCCTCCACCGCGTCGTAGTGCGCAGAGAACTT39930.4415853278215345No Hit
CCCCGAGCTCGCTCCTCCGGGGCATTGTCAATCTCCTCGTACTTCTTGAA39740.439484120401397No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA38190.42234269144764347No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC36110.3993399996903484No Hit
CATGCAAGCTGAGGTTCTGAGCCCTGGCACATACTTCATTGAAACTTCAC31540.34880043174282993No Hit
AATTTAGGAAGAGAAATGCAAAAGCAGCTGCAGAATCCTGCCCCTTCCAC31320.34636745473003905No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA30890.3416120905686752No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26410.2920678313991166No Hit
GTCTGAGTGTTAATGATGCCTTTGTGACTGGCGAGTGGGGCCGAGCCCAC25360.28045589565625134No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC24030.2657474437152886No Hit
ACGAGAGAGTTGGCAGTACAGATCTACGAGGAAGCCAGAAAATTTTCATA23740.2625403376529734No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT23590.260881489689707No Hit
GAGTACATGGGTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTT22090.24429301005704224No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG21740.2404223648094205No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA21260.23511405132696778No Hit
GCTCTGACTGGGCTGACTGTGGCTGAATACTTCAGAGACCAAGAAGGTCA21200.23445051214166118No Hit
GTACATGGGGTCTTCTCTGCAGTGGGAGCAGCTCTCCTGCCACAGCTCCT20700.22892101893077296No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC18780.20768776500096212No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC18400.20348535016068708No Hit
GTTCTACAAAGGTATCCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTC18010.19917234545619428No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT17030.1883345387628533No Hit
CTGTCAAGCCAGCCTGCCGAGCCGCTCCTGCTCCAAACCAGCCGCGTCCC16890.1867862806638046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.1864545110711513Clontech SMART CDS Primer II A (100% over 21bp)
GCATAGGCTAGTGGGCAGGTCCGCACAGTCGAAGCCACACCTGGTCTGTT16750.1852380225647559No Hit
GATACGGAGATGGTGGGTCCAGTTTCCAGAGCACCACAGGGCACTGTGTA16520.1826944556877473No Hit
GACTGAAGCGTTCCCGGCTGAAGGTGAGGTTCTGCACCAACGAGTCGCAG15800.17473198546406823No Hit
GTGTAGAGGTATTGCCAAGTGACTCAGAGGCCCCAGACCTAAAGCAGGAG15540.17185664899440636No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA15520.1716354692659708No Hit
ACATCTCAGTGAGACCAACGGAGAACAAGTTTCTGTAGTTCTCCAACATC15170.16776482401834905No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG14970.16555302673399377No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14830.16400476863494506No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14400.15924940447358119No Hit
ACTTACAGTCCCAGCTGTGTGCAGAGCAGGCTCAGCAGCAGGCAAGAGTG14350.15869645515249237No Hit
GGGCTGGGAGAGGCTCATGGGTAGCAGGGCAGCAGGCTTGCCCTGGCAGA14190.15692701732500813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.15692701732500813No Hit
GGCCAGGGTCAGAAAGTGCAGAAGGTTATGGTGCAGCCCATCAACCTCAT14150.1564846578681371No Hit
ATAAATAGCAGTACATCTTGACCTTCTTGGTCTCTGAAGTATTCAGCCAC13780.15239283289207978No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13720.15172929370677318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13530.14962808628663565No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC13230.1463103903601027No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC13180.1457574410390139No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC12890.14255033497669872No Hit
CTTGCGGATCCCGTCAGAGAGCTTCTCCACAGCCATCTGGTCCTCGTTGT12890.14255033497669872No Hit
TCCCGAGCTCGCTCCTCCGGGGCATTGTCAATCTCCTCGTACTTCTTGAA12660.14000676809969012No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12430.13746320122268155No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA12260.13558317353097954No Hit
GGCTAGGTGTGGGGAGCAGCACTGTGGAGAGGGCAGGGAGCATGTCTCTA12090.13370314583927753No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11990.1325972471970999No Hit
ATGTTGGCTACCAGGGTATTTAGCCTAGTTGGCAAGCGAGCAATTTCCAC11710.1295007309990025No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCC11640.12872660194947813No Hit
GGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGCGCTTTCTTCTGAGGG11590.1281736526283893No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC11460.12673598439355835No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11390.125961855344034No Hit
GCCCAGGAGTTCAAGGCTGCAGCAAGCTTTGATTGCGCCACTGCACTCCA11380.12585126547981623No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11370.12574067561559849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11340.1254089060229452No Hit
CCGCGGTTCCCCATCCCAGGGCCACCAGGGCCCCCGGGCCCCCCCGCTGC11280.12474536683763861No Hit
GTCATGGTACTTGATGTTATTGCATGCCCATTGCTGCAAGGCATCACACA10950.12109590131845235No Hit
GAATTAGAAGTGTGCCAGCTTCAAAAACCCGATGTGGTGGGTATCAGGAG10910.1206535418615813No Hit
ATTATACCCTCTGCCAGCCCCCGGCCTATCATAGGGACCTGGCCGCTGCA10790.11932646349096813No Hit
CTATGCAGATCCGGAGTGCGTCCCCAGTGTACACATCTCTCTCAGCCGCA10700.11833115471300824No Hit
TCCCTGTGCTCCTGCACCGGTAGAGTTTTCCTCTTCTCTGGGTCCGGTCC10530.11645112702130625No Hit
GCTGTCAGCAGCAGCTGGCGGTAGAAGCGCCGGTTCTCCTCGGTGTTCTC10430.1153452283791286No Hit
CATCTGTGGTCCTCTTCAGGCCATCCAAAATGGATTCTCGGCAGCAGTAC10090.11158517299572458No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA9910.10959455543980484No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9350.10340152304361No Hit
GTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCA9340.10329093317939225No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG9260.10240621426565012No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9160.10130031562347247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)