FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833988
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT498605.978503287817091No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA188462.2597447445286982No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA81820.9810692719799325No Hit
TCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCA78080.9362245020312043No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG74440.8925787901024955No Hit
ATGCTGAGGAGGTGCCTGTGTGCACCCAGGAGCAGACATCCAGGAAATCA66390.7960546194909278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57860.693774970383267No Hit
GCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGCTCACTC56030.6718322086169105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52180.6256684748461608No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC44930.5387367683947492No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG33570.40252377732053696No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC32640.3913725377343559No Hit
GACAAAATCTCTCAGCATTTGCTTGTCTGTAAAGGATTTTATTTCTCCTT28800.34532870976560814No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27750.33273860055540366No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC27710.3322589773473959No Hit
CATTAACTTTCTTCTGCACATAGAGAATATTAACCCTTAATGATATACGT26510.3178702811071622No Hit
CACTGAAGGCCTGAGGAAGACGAGATGTCACAGAGGTCCACTCAGTGACA24880.2983256353808448No Hit
GCAGGCGGATCACGAGGTCAGGAATTCGAGACCATCCTGGCCAACATGGT22890.27446438078245733No Hit
CCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT22230.2665505978503288No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21430.256958133690173No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.23405612550780108Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT19260.23093857465575043No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA19010.22794092960570175No Hit
GCAATGGTGATCTTCTTGCTGGTCTTGCCATTCCTGGACCCAAAGCGCTC18850.2260224367736706No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA17360.20815647227538045No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17250.20683750845335905No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT16830.20180146476927724No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16750.2008422183532617No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16680.20000287773924802No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA16280.19520664565917017No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT16180.19400758763915069No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG15170.181897101636954No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC15130.1814174784289462No Hit
TTGTTGCTCTAGCAGAGAGAGACTAAACATTGTCGTTAGTTTACCAGATC14320.1717051084667885No Hit
GTGCACCTGTAGTCCCAGCTGCTCGGGAGGCTGAGGCAGGAGAATCGCTT13940.1671486879907145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13710.1643908545446697No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC13580.1628320791186444No Hit
TGGCTGCTGAGCATGATGTTCTCGGGCTTGAGGTCCCGGTAGATGATGCC13450.16127330369261908No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13170.15791594123656458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.15431876717650614No Hit
CCTGAAGGCGACCCACGGGGGCCGCATCGACTACATCGCAGGCCTAGACT12420.1489230060864185No Hit
GAATGATGACTTTGAAAACCACTGGCTTACGCCCACCATTTCCGAGGTCC12350.14808366547240487No Hit
CTCCATCTCTACAAAAAATTAAAAAATTACCTGGACGTGATAGTGCATGC12210.14640498424437762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12070.14472630301635034No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA11210.1344144040441829No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11070.13273572281615562No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT10950.13129685319213227No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10740.12877883135009136No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC9960.11942617879393948No Hit
GCCCCCGCCCCCAGGCCCCGCTCCACCGACCGCGGGCCGCGCTCACTCGC9850.11810721497191806No Hit
GCCTGGCCATGCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGAGCTCA9730.11666834534789468No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9530.11427022930785576No Hit
GAGTACATGGGTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAA9510.11403041770385186No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9390.11259154807982848No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT9220.11055314944579539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9210.11043324364379344No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC8920.10695597538573696No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8820.10575691736571749No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8810.10563701156371555No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC8470.10156021429564935No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)