FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495058
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC308296.227351138654461No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA87621.7698936286253328No Hit
CTTCTGGGACCTGCCAGCACCGTTTTTGTGGTTAGCTCCTTCTTGCCAAC51261.0354342319485799No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG41180.8318217259391828No Hit
GTACATGGGACACTCGCTTCTGGAACGTCTGAGGTTATCAATAAGCTCCT35250.7120377814316706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.6352790986106679No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA30580.6177054001753329No Hit
GAGTTAATCGGACCAGTCGTCCTCATCAAATTCAGAGGAGTCATCTTCTG29080.5874059201144108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.5851839582432765No Hit
CTGCTGCCCGCGGCCGCGGGAAAGATACCATTGCGCTTATCGGGCAGAAC26750.5403407277531118No Hit
GTGACTATGAGCTCAAATACTGTTTTGTGGACAAATACAAAGGGACAGCC26590.53710878321328No Hit
GGGTTGGAGCTGTGAAGGACCTTGTTGAAGACCTCTACAATCAGTGGGGC24310.4910535735206784No Hit
GTCTAGATTTTAGTCGAATGATGGTATTGTCTTTTTCTTTACTGCTTGTT21410.43247457873622885No Hit
GCACAATGAAGATCAAGATCATTGCTCCTCCTGAGCGCAAGTACTCCGTG21350.43126259953379203No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC20330.41065895309236494No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC20000.4039930674789621No Hit
GTCTAAGGGACTTGCTCCTGATCTTCCTGAAGATCTCTACCATTTAATTA19920.40237709520904624No Hit
GGCCGAAGGAGGACGATTGAAGTAGGGCAGCACACTGGGGGGCTTATCCA18570.3751075631542163No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18400.3716736220806451No Hit
GCCCGCATGGTTGGGCAGTTGGTTGAGGCCATGAACAGAACTTACGGTAA17790.35935183352253675No Hit
CCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCC17080.3450100796270336No Hit
GCTTGTTTCTCCTGGGAGCGCTCTTCCGAGGATATTTGGGCTGTCTCCGG17000.3433941073571178No Hit
CTTTGAAGGCGCGGCGAGCGGGAACAGCTCTTGAGGAGTGAGACTGCAGG16880.340970148952244No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15830.3197605129095985No Hit
GGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGC15770.3185485337071616No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA14560.2941069531246844No Hit
GGGACAGACCAGGTGGTGGGACTCGGCCTCGTCGCCGTTAGCCTGATCAT13680.27633125815561005No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12810.2587575597202752No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT12350.24946571916825908No Hit
ACGCAGAGTACATGGGGATGCGCGAGCGCCTAGCTGCGCGCAGCCACCCA12180.24603177809468788No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG12070.24380981622355358No Hit
TTCATATCCTGTAGTTCTTTCTCGTCGTCGACTGCAGGGCTGAGCAAGCT12040.24320382662233517No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11330.228862072726832No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11310.22845807965935302No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11030.22280217671464758No Hit
GTTGTGTATTTGGAAACAGAAACACTAGTGACTCGAGAAGCAGTTACAGA10990.22199419057968967No Hit
CTGGAGGTCTCCAGAGAGCTAGCTGCCGTGGTAACAGGGGCTCCACTGGT10610.21431832229758938No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10170.2054304748130522No Hit
GGGTACGTCGCGCGGAGGTCAGAAGAGTCGCTGAGGACCGGGTGCGGCGG10040.20280451987443895No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT9750.196946620395994No Hit
GAGCCACCCTTCTCCCACTTGAGCTCAATCTCCTCCTCAAAGAGCTGCTC9710.1961386342610361No Hit
AACCTGGACATCAAGGTGACGGAGTTGCGGGGCCTGGGCTCGCTGGCTGT9690.1957346411935571No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC9580.19351267932242283No Hit
ATCAACCTCTGCTGATCAGGAGGGATGCCTTCCTTATCTTGGATCTTTGC9290.18765477984397788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.17876693235944072Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8320.1680611160712482No Hit
TCCCTCAACCGGGACGAGGGTAGAATACGGCAACGTGAACGAGCTTCGAG8160.16482917153141652No Hit
TGCCGAAGGAGGACGATTGAAGTAGGGCAGCACACTGGGGGGCTTATCCA8090.16341519579524016No Hit
GTGGAGCACTGATTGTTGCTTGGAAACCTATTGATTGTGGAGAATCCATG8090.16341519579524016No Hit
TGGTGATGCGGCCCAACAGGAAGCAAAGCACAGGGAAGCAGAAATGAGAA8060.1628092061940217No Hit
GCTGGTAATCTGAGAGAAAATATCGAGTTGGGAAATGGTCCACTAGAGGA7920.159981254721669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.15816328591801365No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7800.1575572963167952No Hit
GTTTCTGATCTTCGGATTCTTTCTTGGCTTGCTCCAATGCCAGCTCCTCA7710.15573932751313987No Hit
GGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGG7540.1523053864395687No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT7320.1478614626973001No Hit
GAAGTAGCTGCAGCACTGGCTGAGGCTGGAGTTGCAGTGTTCGCTTGGAA7300.14745746962982115No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC7200.14543750429242636No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC7160.1446295181574684No Hit
GAGAAGGGTGCGGGCTCTTCGCCCTTTGTGTCCTCCTTCTTTCACTAACT7050.14240755628633414No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC6970.14079158401641828No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6890.13917561174650242No Hit
TCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCC6830.13796363254406555No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6830.13796363254406555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6820.13776163601032607No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA6800.13735764294284708No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC6780.13695364987536812No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC6760.13654965680788916No Hit
TCCCTAGGCCCCTCCCCTCTTCAAGGGGTCTACATGGCAACTGTGAGGAG6530.13190373653188112No Hit
TTCATCAGCAGGTTGAGCAGGCTGTGCCTTTCTCTGTGGTCTTTCTTCAA6460.13048976079570476No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC6230.1258438405196967No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC6120.12362187864856239No Hit
CCGGGTGCCCCCTGCGGAGCCCCGAGGCCGAGCTCATTGCAGCCAAACGC6110.1234198821148229No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA6100.12321788558108343No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6030.12180390984490708No Hit
GTCCACTGGGAGCATTGCCAAGCGGCTGCAGTCCATTGGCACCGAGAACA6020.12160191331116758No Hit
AACTCTGCCTCCCGCTCAGAGTCCCCCCAACCCTCACTGTTTCCCGTTGC5970.12059193064247017No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5920.11958194797377278No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAC5880.11877396183881483No Hit
GACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGC5870.11857196530507537No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC5860.11836996877133588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5700.1151380242315042No Hit
TGGTTGGAGCTGTGAAGGACCTTGTTGAAGACCTCTACAATCAGTGGGGC5640.11392604502906731No Hit
ATACTGCTCCACCTGGTCCTCATGCTGGGCCCGCAGTTCCTGCAGCGCAT5600.11311805889410936No Hit
CACAAGAAGCCAACCATCTGCAAGCGAGGAAGACAGCACTCCCTAGGAAA5560.11231007275915145No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT5480.1106941004892356No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5470.11049210395549613No Hit
GATGGGCTCATCCGAGTCCCACTGCTCCACCTCACAGTCTGCCGCCCGGG5430.10968411782053819No Hit
GTTATGGCTTCTGTGAGGCCCATTTTCCCTTTTCCCCAGCTGATGAGGAG5400.10907812821931975No Hit
CTGCAGCTCTTCAGACTGTTTACGGGATCTGCTCACTTGGGTCTCTAGCC5330.10766415248314339No Hit
GCTTCCAGCTGTGCCTCTGCTGCCAGCCAAGTCTTCATTTGGGGCCAAAG5300.10705816288192493No Hit
CTTTCTTCTGTCTGGGGCAGGTTGAAGGCATTCAAGGTGGCCCTCTTGGA5270.1064521732807065No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5180.10463420447705117No Hit
CACACACACTGTGGCCAGGCATTACGCCAGCTCCCGGATGCCTCAGCCTC5180.10463420447705117No Hit
GCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT5120.10342222527461428No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG5040.10180625300469844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)