FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552062
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT478138.660802591013328No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA126822.297205748629683No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61011.105129496324688No Hit
CAGTTGATCCAGGAAGGTCTCAAAAGTGGTTTTCTTTATCCACTTTTTGA54620.9893816274259051No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA47530.8609540232799939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33570.608083874637269No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG29850.5407001387525314No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC28710.5200502842072086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27900.5053780191355318No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25870.4686067869188606No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25320.4586441377961171No Hit
CTGCAGGCCGAAAGAGCCGTGGCCTTGGAAAGGGCCACAAGTTCCACCAC22770.4124536736815792No Hit
AAATAGTATTTCAAACTGTACAGTCACCAGAAGTACACAGTTATCAAAAA22740.4119102564567024No Hit
CCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTA22680.4108234220069485No Hit
GCCGCCATGTCTCTGACCAAGACTGAGAGGACCATCATTGTGTCCATGTG21040.3811166137136771No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC20920.37894294481416946No Hit
GTCAAAGGCGTTTGAACCACAGCAACTCCATCTTGATTAGGAGCTGGCTA20530.3718785208907695No Hit
TGGTGGAACTGTCCAAGTCTCAGGATGATGAAATTGGAGATGGAACCACA18450.3342015932993033No Hit
GCTGGAAGGCCAGGTTTTCATCTCAGGGTGTCGAATGAATAACGAATGCT18360.3325713416246726No Hit
GAATGCAGGTGTTCATTCCGGTGGGCTCGCTTTATTCTTCCAGACTGCAT17960.3253257786263137No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT15850.2871054338099706No Hit
GCATGAAGCTCCAGCCTTTTCGTGGTGACTCAATAGAGCAATTGTACCTT15270.27659936746235025No Hit
CAGCGGTACTTCTCGGTCAGGACAGAGGATACGACCGATAGGAACTTGTC15230.2758748111625144No Hit
CCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCC14960.27098405613862214No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT14860.2691726653890324No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA14470.2621082414656325No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT14140.2561306519919864No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14070.2548626784672736No Hit
GTCATAAAGATAAACCTGGTATAGTAGGACAAACAGGCAATCTATTGGGA13990.25341356586760183No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13550.24544344656940706No Hit
CGTGATGGACTCCGGTGACGGGGTCACCCACACTGTGCCCATCTACGAGG13340.24163952599526867No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13110.2374733272712123No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG12970.23493738022178667No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11780.21338183030166902No Hit
GGCAGAGGACGATAAGCACTGCTTCCCGCAGGCATTTTAAAAATAAAGTT11760.21301955215175106No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11380.2061362673033101No Hit
ATGGTGAGGTGGCTGGTTCTGTTTGTTTTCCTCTTAATTGTCTTCTACCA11200.20287576395404866No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10920.1978038698551974No Hit
AGCCAGGACTGTCTCTTAACAGTGCTGCCGAGGGAGTATGGAGTGTGAGG10690.19363767113114105No Hit
GACCAAAGCCATTAAAGACCAATTGCAGAAATACATCAGAGAGCTGGAGC10650.1929131148313052No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.18947147240708473Clontech SMART CDS Primer II A (100% over 21bp)
GCTCCTTGATGACCGCGCAGGCCTGCGCCGCGAAGGCTTCGTTCGACTCG10390.18820349888237192No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT10330.18711666443261807No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10080.18258818755864376No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9530.1726255384359003No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9350.1693650350866388No Hit
GTACATGGGAGAGAGTGGGGACGTCCGGCTTCGGAGCGGGAGTGTTCGTT9310.16864047878680294No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9250.16755364433704908No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAG9020.16338744561299273No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9000.1630251674630748No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8950.16211947208827995No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8940.16193833301332097No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8670.15704757798942873No Hit
ACAGTGGCAACTCCACCGATTATAATCCAAGGGTTGACCACCGTTTGCCC8490.15378707464016722No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8360.1514322666657006No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8340.15106998851578265No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8160.14780948516652115No Hit
GGGATCGGTGGTGTGTAATGTGGCCCTTCCTTCTCAAAGGGGAACAGGTT7810.14146961754295712No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA7630.13820911419369564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.13712227974394178No Hit
GAGTTGATTTCCCGGTCCCCTTTTCACCTGTACAAAGACAACGAAAGTTC7480.13549202806931107No Hit
GATCAGGAGAATCCTCAAATCCAGGCAAAGGGGTAGGTAATGACATCTCT7410.13422405454459826No Hit
GTTCACGGGGTCGTTTGTCATTGGTTCTAGCAAATTAATGGCTTCCTTGT7380.13368063731972135No Hit
GGAAGAACTGCATTGACATCACGGGAGTGCGCCTGCCCACCGGCTACTAC7260.13150696842021367No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7250.13132582934525472No Hit
GCGGAGGTGGTGGCGGCGGAGGCTTTGGCAGCTCGGGACTGAGTGCAAGG7050.12770304784607525No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7010.12697849154623939No Hit
ACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAA6910.12516710079664967No Hit
ACCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTA6870.12444254449681376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6820.1235368491220189No Hit
AGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAAATGGCGAGACTACC6810.12335571004705992No Hit
ACTCGGATCTTGGCTGACATTTCCAGAGGCCTGTGCTTATCTGCAACACA6720.12172545837242918No Hit
TCTCTGTACAGCTCATCCACTTCCTCATCTGTAAACCGATCCCCCATGGT6510.11792153779829077No Hit
GGCCAGATGGCTGGTCTTGAACTCCTGACTTCAAGTGATTCACTTGCCTT6510.11792153779829077No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC6500.1177403987233318No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT6380.11556672982382414No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6370.11538559074886516No Hit
CCTCAAGATGTGAGTGAATTGCTGCAATCTCATGATAAAACTTGAACAGA6360.1152044516739062No Hit
GAATTTGTCCCTTCTAGATCAACTGAGGAAACTCCAGGCCATGGTGATTG6330.11466103444902928No Hit
GGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA6160.11158167017472674No Hit
CAGCACTTTTGGAGGCTGAGGTGGGCGGATCATTTTTGCTCAGGGCTTCA6150.11140053109976779No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT6150.11140053109976779No Hit
TCTCAAAGTCCACCATGGCAAGCTCTGAATAGCTGTCAGTTTATGTCTTA6140.11121939202480881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6070.10995141850009602No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6050.10958914035017807No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT5970.1081400277505063No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG5960.10795888867554732No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT5840.10578521977603966No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC5820.1054229416261217No Hit
GTACATGGGCAGGTAGATGGAGCCTTTGGGGAGGGAGGGGTGGTCAGTGA5660.10252471642677814No Hit
CAACAAAACAAGCCTCAAAGCCAGAACAAAACAAAACAAAACAAAATCGA5650.10234357735181918No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC5600.10143788197702433No Hit
AGATGGGCCCGAGCGCCCAAGCTACCTCCACACGGTGGCCTGAGTCACCG5530.10016990845231151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)