FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760259
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA385405.06932505896017No Hit
ACATGGGGTACTTCCTCTTTCCCTCGGAGCGGGCGACGGCGTTGGCGGCT181372.3856343693399222No Hit
GGACAGGGCTCTCCGGGGAGCGGAAGCGCCTTCAATGCAGGCCTGTGGGC148901.9585430754519184No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA97921.2879821218821481No Hit
GTCAAGGACTGGGCAGATGGCCGTGTGGGGCTCAGACCAGGCCCTGGGAG89591.178414198319257No Hit
CAATAGCAGTCTTCAGCAGCTCCAGGCCTTCTTTATTCTCCAGGATGTTG73990.9732209681174442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.6705609535697703No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG47780.6284700345540138No Hit
GTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGT47620.626365488603226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.5979541182675904No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA35990.4733913048053361No Hit
GTGGAGCATGCCTGTAGTCCCAGCCACTCAGGAGGCTAGGAAGGAGAATC31460.41380634757365575No Hit
GTTTTAGACAATTACTTAACATCCCCCCTCCCAGAAGAAGTGGATGAAAC30900.4064404367458984No Hit
GTTCGGCAGTAACCTACTTTGTTCCTTCGCCTCAGCAGCAAATCTTGCTA29930.39368162691924724No Hit
GGCCACCAGCCTCCGCAGTATGGCTTCCTTCTCTGTCGATGGGGGACCCT28680.3772398616787174No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA27030.35553673156121796No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC26990.355010595073521No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26880.35356371973235434No Hit
CCCCGGAACACGCCTCCGCCTGAGCCTCGGGCCCGTCGGGATCTGCCTTT22840.30042393447496185No Hit
GAGATGGTTGAGCTCATGTTCCCGCTGTTGCTCCTCCTTCTGCCCTTCCT22760.2993716614995679No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT21510.282929896259038No Hit
TGACCCAGATCAGCCCTGGCATGATCAAAGACTGCGGAGCCACGTGGGTG21330.28056228206440176No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC20530.2700395523104626No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA20360.2678034722377506No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG19640.25833301545920534No Hit
GTTCTACGCCTTTGGACGAGTCTTCTCGGGGCTGGTCTCCACTGGCCTGA19620.25806994721535687No Hit
GGGTAAGGGATGAATCCATGATGTCATCTGCCACCAGGAAGAAAGCTTGC19580.2575438107276599No Hit
GCTAAATACTTTGACGCCGGTGGGGATAGCGATGATTATGGTAGCGGAGG18920.24886255868066015No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC18700.2459688079983269No Hit
GCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCA18160.23886596541441799No Hit
ATTTTGGTACTTCTTGCAAGGGATATGCATTAGCACATACTCAAGAAGGG18140.2386028971705695No Hit
TGGAACCCCTTCCGCTCCAAGCTAGCAGCAGCAATCCTGGGTGGTGTGGA17850.23478840763476658No Hit
GTGTAGAACTTGCAGGTAGGAATCAAAGTGATCCCATACAGGGCTCCCAA17840.2346568735128423No Hit
GGCGGGCAGCGGTGGTGCCCCCGTGCTGCGGGTGGGCCATGACCACGGCT17730.2332099981716757No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16870.22189806368619114No Hit
GTACTTCTGAAGATCCAAAATATAATCATCTCCCATTTCCAGCTCAAGAT16560.21782050590653973No Hit
GTGTCAGGGTAAGGAAAGCTGCCTTGGTGGAGGAAGAGAAGTCAGAGAGG15700.20650857142105516No Hit
AGATGGGGTACTTCCTCTTTCCCTCGGAGCGGGCGACGGCGTTGGCGGCT15080.19835345586175238No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.19348669335055554Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14670.1929605568628586No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC14270.18769919198588902No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA14080.18520004366932846No Hit
GCGGCGACCGCGGCGGCTGCTGCTCCGAGGGGCGACACGAGGGAGCGCGC14000.18414777069393456No Hit
GTGAGCGCCCTGTGTTGGGGCACCGGCGGCGAGACCCAGATGCTGGTGGG13750.18085941764582858No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC12750.1677060054534047No Hit
GATTGACTACCCTGAGATCGCCGAGGGCATCATGCCACGTCACCGCTTCA12570.16533839125876837No Hit
GCATGGGGTACTTCCTCTTTCCCTCGGAGCGGGCGACGGCGTTGGCGGCT12430.16349691355182905No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12360.1625761746983594No Hit
CTACAGAAATTGTTGCTTTTAGTGACAAAGCTAACGAATTTCACGATGTG12310.16191850408873817No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11980.1575778780652383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11840.15573640035829894No Hit
GCAGACGACGAGCTGAGCTTCAAAAGGGGGGACATCCTCAAGGTTTTGAA11670.15350032028558688No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11550.15192191082249604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.1474497506770719No Hit
CTTTCACCTGCTGCACAAAATTCTGTGTTTATTCATCTGCTTTCCTTCCA10670.140346908093163No Hit
CGTGAGAGCTGGTACGCGATGTCCTCTGCAGCTTCCAGCTTGCGCAGCTC10670.140346908093163No Hit
CTTGGCTTTCTGGGGCTGAGCGTTGGACCTGGGAAGAGGCCTTTATTGCC10180.1339017361188753No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10100.13284946314348137No Hit
TCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCAGCGTAGGGTAGGC9910.13035031482692083No Hit
CTGTAGTGGGTGTTGGACAAGTTGGTATGGCGTGTGCTATCAGCATTCTG9670.12719349590073908No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9560.12574662055957245No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9530.12535201819379974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9070.11930144858528476No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9060.1191699144633605No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC9040.11890684621951204No Hit
ATACAAAGCATAGCGACAATCTTTTTCAGGAAGCATTCCCACAAAATGCT8970.11798610736604236No Hit
TTCTATGGAAGTATGTGCAGATTTTCCTCATGGAGCTGGGCGTGATGGTG8790.11561849317140607No Hit
ATTCTGTTCAGGTGGGGTCTCTACTGGTTTGGATTTGAAGTAATTGACCA8730.11482928843986062No Hit
GACTGTGGCTCCACCGCAATCCTACCAGTGTCCGCCCAGCCAGATAGGGG8660.11390854958639096No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8630.11351394722061825No Hit
ATCAACGCAGAGTACATGGGCGCCGGGACGCTTCTTCTGTAAGTTACTGC8440.1110147989040577No Hit
AGATGGGCAGATGGCCGTGTGGGGCTCAGACCAGGCCCTGGGAGGGGACT8400.11048866241636074No Hit
TATCTTTACTGTTGTTTGGATGTTGTAAATTCACCCAAGTCCTGACTTCT8100.10654263875863358No Hit
TGCAGAGAAGGCCAGGAGACAATAGATAAAAGTTATTATGACACTGCAAC8050.10588496814901238No Hit
TCCACTAGCAGCAGAGAGAGATTCTGAATCAAAGCCTGGGCCTTGGCTCT7940.10443809280784576No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7740.10180741036936096No Hit
GCAATCGCAGTCTTCAGCAGCTCCAGGCCTTCTTTATTCTCCAGGATGTT7610.10009746678434586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)