FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963967
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT458984.761366312332268No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA119941.2442334644235746No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA64950.6737782517451324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59780.6201457103822019No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT57950.601161658023563No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG54130.5615337454497924No Hit
GAGCGGGTCCGGAGCAGGCTCCCGGACCCAAAACTGAAGGGGGCGTCTGA51480.5340431778266269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.5021956145801671No Hit
TTGCTGGCCCAGTTGCTGTCCTGATATAGAAATTGCCCAGTAACCGGATG46760.485078846060083No Hit
CTTTTTTGAAGGTCTCCTTCCTGAAATCAAGACGTACTAGGTCAGCCCAT44280.45935182428444127No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA39200.40665292484078813No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA38270.3970052916749225No Hit
ACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT33910.3517755275854879No Hit
GGGTGGCCTTATTGGTCAGCTTTTTGGCACCTGTGAGGATGTTTTCGGAC31160.3232475800520142No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC30410.31546723072470323No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC29020.30104764997142014No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26830.2783290299356721No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC25600.265569257038882No Hit
CTGCATGGTTGGGCTTTCCACTGGAAGAGGGACTTAAAGCCAGCATTTTG25220.2616272133797111No Hit
CATTGAGGCTGGGAGCTCCCACTTTGTCCCCGTCTTCGTGGCGGTGTTAC23520.24399175490447286No Hit
CCCTTAGCAAGGCTGAAAATTTCAGTCTCTGGTATTTGGAATTTAGGCTG23090.2395310212901479No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22960.23818242740674733No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22240.23071329205252877No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG22130.2295721741511898No Hit
GTCCAGCCTTCTGCTGGAGCACTTGCTCAGCTCCTGGGAGCAGATTCCCA19070.19782834889576095No Hit
TCTTAGGGCTCTGGGCTTGGCTGAAACATGACGGTATTGCTTGGTTTCAG18740.19440499519174412No Hit
GGCTTCAACTGCGGCAGCTACGGCTAGGGCGACGGAAGAACTCCCACCAG18400.19087790349669648No Hit
GTACATGGGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTC18020.18693585983752556No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17290.1793629864922762No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG17060.17697701269856747No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT16970.17604337077929016No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG16690.17313870703042739No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16230.16836675944300997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.16349107386456177Clontech SMART CDS Primer II A (100% over 21bp)
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT15700.16286864591837688No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC15330.15903034025023677No Hit
GTACATGGGTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCT14970.1552957725731275No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14850.15405091668075777No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA14840.15394717868972693No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14480.15021261101261765No Hit
AACTAAGCCCAGAGAGGGTAAGATATTTACCAAAGATCACACAGTCAGTG14100.14627056735344673No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14080.14606309137138512No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13180.13672667217861192No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT13070.13558555427727298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.13392574642077998No Hit
GTCCCCGCTTCTTTTGTGCCTAAGCCTGGGTCTCTGAAGAGAGGCCTCAA12790.1326808905284102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12690.13164351061810206No Hit
GCCCAGGGAAGACAGGGCGACCTGGAAGTCCAACTACTTCCTTAAGATCA12640.131124820662948No Hit
GATCAGAGAAAGGGATCAAGGGGGTTGGGAGGGGGGAAAGAGACCAGCCT12550.13019117874367067No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA12530.12998370276160906No Hit
CTAGAAACACCTGAGAAGCCTGAATGCATGCAGGCTTGCAAAGCTAAGGC12390.12853137088717767No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC12340.1280126809320236No Hit
TACCACACCTGGCCCCACGTGCCTCTTTCCCAGACGCTAGAATGCGTGGC12280.12739025298583873No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12110.12562670713831492No Hit
GTGTCCTTCTCTCTTCCAGGGATATATCCTCATCTGGATGGATCAACTTA12060.12510801718316084No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG12040.1249005412010992No Hit
GTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATT12010.12458932722800677No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT11640.12075102155986667No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAG11420.11846878575718879No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT11340.11763888182894228No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11110.11525290803523357No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG10980.113904314151833No Hit
GCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCG10850.11255572026843243No Hit
GTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT10780.11182955433121672No Hit
GGGGATATACAGTGGCTGCACCAAATTGGAGGTGTGGGTTCCTCCAACAC10530.10923610455544641No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG10450.1084062006271999No Hit
ATCCAACCACTCAGTCTTGGCAGTGCAGATGAAAAACTGGGAACCATTTG10310.10695386875276851No Hit
GACATGCCTCGTATGTGTTAGAGGGTGGAATGGATGTGTTTGGCGCTGCA10250.10633144080658362No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10160.10539779888730631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10100.10477537094112142No Hit
GTGCCACTGTTTGCCCCACTTCAAGATGAACAGCGCAGCTCCCAGCAACA9950.10321930107565923No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC9810.10176696920122784No Hit
GTTCTCCTCTGCTTTGGCAAAAACTCCAGAATGGTGTTGATGCTTGCCTC9770.1013520172371046No Hit
CCACGGAAATGCCACGGCCAAGAGCCCAGGTCCTTCACGCTGGCCTCTTC9720.10083332728195052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)