FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986202
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA703037.128661268178324No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA177981.804701268097205No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG88050.8928191181928246No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA58760.5958211400909753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.4902646719434761No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46790.47444641158707856No Hit
ACATCAAGAATTCAAACCCAGCACTCAATGACAATCTGGAGAAGGGACTC46060.4670442769331233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42190.4278028233566754No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC38780.39322572860326793No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG36630.3714249210607969No Hit
GTAGTCTCCTTCGCTCTGTGGCCCGATGCTGTGCATGCTGAATCCGCGGA31560.32001557490250476No Hit
GGATTCACCCTCCTAGCGGAAGGGTATATAACCTGGACTTCAATCCACCT30910.3134246330873391No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA26390.2675922376957256No Hit
CGCGCAACTGCGCGGACCCCGGGTGCAGGTCGAAGTGCGGGAAGTAGGTC21920.22226683782835566No Hit
GCTCTGGCGGGCTGGAAAACGGCTCGATGCACAGGGCCTCAAAGGTGTCA21420.21719688258592054No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG20030.2031024070119509No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCA19370.19641006609193656No Hit
CTCCATGGTGCTGTCATAGGCAGAGCGGAGAGAGGCAAAGTTGATGAGCA19280.1954974741482982No Hit
GGGAGGATCGATGCCACGTGGGCCCCAGCTCACCCGGGTGGAGGCTGGGA18880.19144150995435014No Hit
GTGGTAACAATCTCTGATGAACCAGACATATTATATAAGCGCCTCTCGGT18650.18910933054282997No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18450.18708134844585592No Hit
GAGACAGGGTTTCACCATGCTGGCCATGCTGCTCTCGAAGTCCTAACCTC18330.18586455918767147No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC18080.18332958156645393No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA18050.1830253842519078No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG17590.1783610254288675No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17460.17704283706583437No Hit
ATAGTCACCTCTGCAGCAGCTCATTAATGACTGGTAACACATAGAGGTCA17230.17471065765431423No Hit
AAACTATGAGGACGCAAAGGCATAAGAATGACACAATGGACTTTGGGGAC16170.16396235254035177No Hit
GCTAAGCCCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTA15790.1601091865561011No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15520.15737141072518612No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.144797921723947Clontech SMART CDS Primer II A (100% over 21bp)
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14250.14449372440940092No Hit
CACCAGCTCCTTGCCCTGCGCGGCCAGGCTGCTCCCATAGGCCATGGCCA13970.14165454947363726No Hit
GCCAAAGGCCCGGCCTTCTGATAGACTGAAATTGGGGTGGTCCCCATTTC13650.13840977811847877No Hit
GAAGATAAATGTATCAAAAGAACACACAGGTGGCGGGGGAGGCACAGCCA13120.13303562556149753No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13050.1323258318275566No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA12430.12603908732693708No Hit
ATGTGTGCCCCACAGCAGAGCCCACAGCCACGCCAGCTGCAGTGGTTGCC12200.12370690791541693No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT11930.12096913208450195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11920.12086773297965325No Hit
GTCAGTTCCCGAATCCGCCGGCCCTTCTCACCAAGAACATTCTGTGTTCT11690.1185355535681331No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11340.11498658489842851No Hit
GAGGGAACTTTGCCCACCTTTGAAGATCAAATCAAGAAATCAGGGAAAGC10940.11093062070448043No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10830.10981523055114469No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC10550.10697605561538102No Hit
CTCATGTGTGCATCCAACAAACTGCCCTGGAGCTAGTTGGCCGAGGAGTC10420.10565786725234791No Hit
TTTCTGAACTCATCACAGTCACAGAGGATCAAATTCATGTGCTTGTCAAA10340.10484667441355829No Hit
GGGATAATGAGCCCAGCACGCCAGTGCCCTCACCCCGAACCTGCAACTCT10190.10332568784082775No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10170.10312288963113034No Hit
ATCTTAAGGGGCATGTGCATTCAAACACATACATGTACATGGCAAGATGA10170.10312288963113034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.10221029768749201No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG10050.10190610037294591No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA10040.1018047012680972No Hit
GTAAGACGCCTCCGTACTGCTTGTGAACGTGCTAAGCGTACCCTCTCTTC9970.1010949075341563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9880.10018231559051796No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA9880.10018231559051796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)