FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825801
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC620667.515854303881928No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA154821.8747858140157252No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG74230.8988848402944535No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA50710.6140704600745216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.6022031942320244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.5188901442357178No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38750.4692413789762909No Hit
ACGCAGAGTACATGGGGACTCTCTCGCGGTAATTCGAAGTACCGCGCCTG38390.46488197519741437No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA34040.4122058462026566No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC32460.3930729073953652No Hit
GGAGAGCACCCCTCCACCCCATTTGCTCGCAGTATCCTAGAATCTTTGTG31380.3799946960587357No Hit
GAGAAGAATCAAATAGACACAATAAAAAATGATAAAGGGAATATCATCAC31050.37599857592809893No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT30190.36558444467856055No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30040.363768026437362No Hit
CTCCAAGTCAGCATGGGGTGCTGGGGGCCTGCCCGGGTCCCTTCACCAGA29690.3595297172078988No Hit
GTCTGTGACTGGCCTGGCTGGAGGGGGAGTGTTGACAACCCAAAGCTGTT28420.3441507094324177No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC28280.34245538574063245No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT27100.3281662289098705No Hit
GGCCGACACCATCGGCACCGAGACTCTGGAGAGGCTCTTCCTCAGCCACC26320.3187208540556381No Hit
AAGTTACAGAGCGCCTGCCCGGCCGCCAGCGCTCAGAGGGGAGGCGCAGT25690.31109189744260424No Hit
AATCTGGTGGCATCACACAGCAGGAGGCAAGATTAACTTGGTTACTATTT23840.2886894058011555No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA23660.2865097039117172No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC22140.2681033324009053No Hit
GAGAAGAATCAAATAGACACAATAAAAAAATGATAAAGGGAATATCATCA21550.2609587539855243No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG19470.2357710877075712No Hit
GCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCA18860.22838432019336377No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18730.2268100910509917No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA17650.2137318797143622No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC16840.20392322121189002No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16340.1978684937412282No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA15920.19278252266587229No Hit
GAGTAAACAATATGGTAATGAAGTATTTCTGGCCAAGCTTATTGCTCAGG15440.18696998429403694No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC15400.18648560609638398No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC15270.1849113769540119No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14800.17921993313158982Clontech SMART CDS Primer II A (100% over 21bp)
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT14560.17631366394567216No Hit
GACGGACAGTGTGGTGGCAACATTGAAAGCCTCGTACCCTGGCCTGCAGT14500.17558709664919273No Hit
GTGGCGCACAGCCCAGGCATCCTTATCACCATCACCACTGGCAAAGCCTT14140.17122769287031622No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14120.17098550377148974No Hit
TTTTGGAGGTGCTTCTCATGCAAAAGGAATCGTGCTGGAAAAAGTAGGAG14080.1705011255738368No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13700.1658995326961338No Hit
AAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGT13640.1651729653996544No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13400.1622666962137367No Hit
CCTTGGGCTGACCGGGTCCTTCTTGTTTCTTCTGTTCAAGTTTCATCTTC12660.15330569955715723No Hit
GGCAGGCAGAAATGCTCGATGACCTCATGGAGAAGAGGAAAGAGAAGCTT12290.1488252012288675No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12230.14809863393238806No Hit
GCCTGGGACCTCTGGAGCACCCTCTGCATGATCCTCTTCCTGATGATCGA12040.14579783749353656No Hit
GTTTTTACATTTATTTAAACAGAAAACGTGCACATGAGCTGCCTACTCAT12030.14567674294412333No Hit
CTGCAGCAGCGCAAGCAGGCTCTGGTCAGCGACCTGGAGACCATTTGTGG11930.144465797449991No Hit
GAACAAGGAGCGGGAAAAGGAGAAAGAGGAGCCCCCGGGTGAGAAAGTTG11730.14204390646172627No Hit
TTCGGTACCTTCGCCCTCGGGGCCTTCAGCGGAGGCATTTCCACCAATGA11530.1396220154734615No Hit
CTGCACAAGAGAGCAAATATAACCACTCAAGCATATTTCAGGCATCTGCA11080.1341727607498659No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10960.13271962615690705No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10890.1318719643110144No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA10850.13138758611336143No Hit
AAATAAGATCCTCACTTTGGCAGTGCTTCCTCTCCTGTCAATTCCAGGCT10760.13029773516864235No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA10590.12823912782861732No Hit
ATCTAGAAATGGAACCACTGCTCTTTTGACAGGTGCCATCTCAGTGGCAT10590.12823912782861732No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT10510.1272703714333114No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10420.1261805204885923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10070.12194221125912902No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC10000.12109454941323637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.12097345486382313No Hit
TTTTTGTATTTTTAGTAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCT9820.1189148475237981No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9760.11818828022731868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9620.11649295653553338No Hit
CTCACGTAGAGGTGGCCCCATTTTATACATGCAAGAAAATCAACCAGCTT9620.11649295653553338No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC9400.11382887644844218No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9270.11225464730607011No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG9190.11128589091076423No Hit
GAGCATGTCCTGTGTTTAATTAGCATTCAGTGACTTAAGGAGGGCTCCCT8860.10728977078012741No Hit
GTTCTACAGCCATTGGGATCCAGACATCAGAGGGTGTGTGCCTAGCTGTG8820.10680539258247448No Hit
GTGCTGTGCCAGGGCTTTGCCTTTGACATCCCAGGATTCTACGATTCCGG8720.1055944470883421No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8550.10353583974831708No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8490.10280927245183767No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA8280.10026628691415972No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG8260.10002409781533324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)