FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589508
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA6589411.17779572117766No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA207223.515134654661175No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG97001.6454399261757264No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA81841.388276325342489No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC53820.9129647095544081No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG48860.828826750442742No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG43650.7404479667790769No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42530.72144907278612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.6444356989218127No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC22900.38845952896313535No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA22630.38387943844697614No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA22590.38320090651865624No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC19140.32467752770106595No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC18610.3156869796508275No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC18430.312633585973388No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA17530.29736661758619054No Hit
TTTCAGGAGCCCCTCAGGTACTCTCACACGGCAGTATCTGTAGACGTCGG15090.2559761699586774No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14840.25173534540667813No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.2507175475141983Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA14680.24902121769339855No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT14620.24800341980091875No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14120.23952177069692013No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG14090.23901287175068023No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC13500.2290045258079619No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13410.22747782896924215No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA11490.19490829640988755No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11090.1881229771266887No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10950.1857481153775691No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10920.18523921643132918No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10730.18201618977180972No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10120.17166857786493145No Hit
GCACAATGGTTTAGTCACTAAAAAATTCAAATGGGATCTTGAAGAATGTA9990.1694633490978918No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC9980.16929371611581184No Hit
GTATAGTTCTTCTCCAGGAACTCGCGCACTGACTTGGCACCCCGGCCTAT9960.1689544501516519No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC9700.16454399261757263No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9190.15589271053149406No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8920.15131262001533483No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8810.14944665721245515No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT8730.14808959335581537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8730.14808959335581537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.14266133792925625No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8390.14232207196509633No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8170.13859014635933695No Hit
GAAACCGATCGACCGCGAGAAGACATGCCCACTGTTGCTACGGGTCTTCA8020.13604565162813737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8000.1357063856639774No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC7600.12892106638077855No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7450.126376571649579No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAAT7410.1256980397212591No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7150.12128758218717982No Hit
GAGCAACCACTCCATGGTTTCTTAGCACCAGGATCTTGCAGGTGGGTCCA7130.12094831622301988No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC7000.11874308745598024No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC6940.11772528956350041No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6860.11636822570686063No Hit
CGTGGTTTTTCTGCCTCCCGAACCAGAGCTGTCTCTTATACACATCTCCG6800.11535042781438079No Hit
GCCAGGGTGACCAGCAGGCAGTGGGACAGGAGCTTGAAGTTGACCGGGTC6620.11229703413694131No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6240.1058509808179024No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT6000.10177978924798307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)