FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676080
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7180110.620192876582653No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA171952.543338066501006No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG92191.3635960241391551No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA60910.9009288841557211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58710.8683883564075257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.7641107561235357No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49270.7287599100698142No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC40700.6019997633416164No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG37300.551709856821678No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC28950.4282037628682996No Hit
GTCCTGTCTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCA24950.3690391669624897No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24900.368299609513667No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT24580.36356644184120224No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT20920.3094308365873861No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA20820.30795172168974083No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA20240.2993728552833984No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC19780.29256892675423024No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17540.25943675304697666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.25425985090521835Clontech SMART CDS Primer II A (100% over 21bp)
GTGGTAACACGGAGCTGCTCAGGGACTTTGTTTCCAACCGGGGTGCATCT16860.249378771742989No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA16790.24834339131463734No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC16110.23828541001064962No Hit
GTGCGTGGCTCACGCTTGTAATCTCAGCACTTTGGGAGGCTGAGGCAAGC15920.23547509170512368No Hit
CCCTAGCTCCATCCCCGCCACCAGGATGATCCAGCCACTCACCTGCCACA15390.22763578274760382No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15040.22245888060584548No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14810.2190569163412614No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14430.21343627973020945No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC13440.19879304224352146No Hit
ACCTAGCTCCATCCCCGCCACCAGGATGATCCAGCCACTCACCTGCCACA13380.19790557330493433No Hit
CAGATCCTGACCTCGTGATCTGCCCGCCTCGGCCTCCCAAAGTGCTGGGA13350.19746183883564075No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12840.18991835285765No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12780.18903088391906284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12760.1887350609395338No Hit
AGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACA12560.1857768311442433No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12530.1853330966749497No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC12470.18444562773636256No Hit
TCCTAGCTCCATCCCCGCCACCAGGATGATCCAGCCACTCACCTGCCACA12220.18074784049224943No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG12120.1792687255946042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.17113359365755532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11470.16965447875991008No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11450.16935865578038103No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT11140.16477339959768075No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC11040.16329428470003549No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA10570.15634244468110284No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10500.15530706425275115No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10210.1510176310495799No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC10160.1502780736007573No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10010.14805940125428943No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA9980.14761566678499588No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACT9550.1412554727251213No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAT9500.14051591527629867No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9060.13400780972665954No Hit
CCTGCAGATGGACGGCAGTTGGTGTGCTATAAATGACCGGGTAGGGTCTG9020.13341616376760146No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC9020.13341616376760146No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8980.13282451780854335No Hit
GTGAAGGAGTCAGCCACAGAGGAGAAGCTGACCCCTGTGTTGCTGGCTAA8950.1323807833392498No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8930.13208496035972073No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT8830.13060584546207551No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8720.1289788190746657No Hit
CTCTAAGAAAACCTTAAGCACACCTCGAGTCTCCTCATAGATAAGACCGG8710.1288309075849012No Hit
ACACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG8590.1270559697077269No Hit
GTACATGGGATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGG8270.12232280203526211No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC7940.11744172287303277No Hit
CTTTTCACAGCAGCTAGCCTCTGTCTTTGTTGTATCATTTTCTCCTCCTC7930.11729381138326825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7850.11611051946515205No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC7500.11093361732339369No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC7410.10960241391551297No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG7390.1093065909359839No Hit
GTACATGGGTTTCCTCTGCCCAGAACAGCCTTCCTTCTTTCCCCTATTGC7250.10723583007928056No Hit
GATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTT7110.1051650692225772No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT7100.10501715773281269No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7100.10501715773281269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6980.10324221985563839No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC6900.10205892793752219No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6800.10057981303987693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)