FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864210
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT9631411.144744911537705No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA264413.0595572835306233No Hit
GTGTCTACATATCCAGTATAGTCCAGAGGGAGCCGTCCGGTCTCGATGAG134461.5558718367063562No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG131131.5173395355295587No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA91231.0556461971048703No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61130.7073512225037896No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59060.6833987109614561No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57970.6707860358014834No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC57870.6696289096400181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51280.5933742955994492No Hit
GTACATGGGAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC46340.5362122632230593No Hit
GTTTCGAGAAGCCCATGAGCACGGCGGACACGAAGGCCAGCAGGTTCATC43900.5079783848833038No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT31740.3672718436491131No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG28970.3352194489765219No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA28220.32654100276553155No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA25300.2927529188507423No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC24850.28754585112414804No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC24750.2863887249626827No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA23500.2719246479443654No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA21500.24878212471505767No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG21130.24450075791763573No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC20730.23987225327177422No Hit
GTGTGTGGACAGCCTGTGGGAGACTCGCACGCCCACACTCACCCCGCCTG19400.2244824753242846No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.21233265062889808Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC16320.18884298955115078No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15980.18490876060216846No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15930.18433019752143578No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15510.17947026764328114No Hit
GAGAAAGACCGCATGAAGATACTTGTAAGCACACATGATCCCTCTGAATT15090.17461033776512652No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT15030.1739160620682473No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14970.17322178637136806No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14690.16998183311926499No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC14660.16963469527082536No Hit
GCCTGAAGAATACCAAGGAGGATCTTTTAGTATTTCCAACTTGGGGATGT14470.16743615556404115No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA14230.1646590527765242No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14180.16408048969579153No Hit
CTATGAAGTTCTAGGCGTGCAGAGACATGCCTCACCCGAGGATATTAAAA14020.16222908783744694No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13460.15574918133324075No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13060.15112067668737922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12400.14348364402170768No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA12140.1404751160018977No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC12090.139896552921165No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT11980.13862371414355307No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11950.13827657629511345No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG11840.13700373751750153No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG11810.13665659966906193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11790.13642517443676885No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11780.1363094618206223No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11710.13549947350759653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.13434234734613115No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG11500.13306950856851923No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10470.12115110910542576No Hit
GCGTAGCTGTGTGGTTGCTCATGATGGGTCTTCGTCGGGGCTCATCAATA10310.11929970724708115No Hit
CCCTGATCTCTCAGTGCACCCCCAAGGTGGACTTTCCCCAGGACCAGCTG10100.11686974230800384No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9920.11478691521736614No Hit
GATCCAAAGAAAACATTCCCCAGGGAAGACAAAAGCAACAAGCTCAGGGC9910.11467120260121962No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT9570.11073697365223731No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG9200.10645560685481538No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC9120.10552990592564307No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8860.10252137790583307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)