FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331314
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC285348.61237376023953No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA83962.534151892162722No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG36531.1025794261636996No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA33921.0238021936893702No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC22860.6899798982234375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.6679464194087783No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18150.5478186855973488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.5294071485056473No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15830.47779447895350036No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC15500.4678341392153667No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA10380.3132979590358391No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA9900.2988101921440084No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA9860.29760287823635584No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT8870.267721859021955No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC8530.2574596908069082No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC8500.25655420537616885No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8200.24749935106877463No Hit
GATCAAATGCGTGAGGTGGCCAGGATGTCACTTTCACTCAGTGACAGCCC8050.24297192391507755No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT7740.2336152411307702No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC7580.22878598550015997No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA7530.22727684311559426No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7500.22637135768485486No Hit
TCACAGTCCCAGAGACGACAGCTGTTGTCAGCTGAGCCAGTGAGGACATG7310.22063661662350517No Hit
AAGCATCTGTTTTATTCAGATCTGGGTGGCTCAGGGCCTCCAGGCAGCCA7280.21973113119276577No Hit
GGACCAAGATCTTCATCCGCTTCCCCAAGACCCTGTTTGCCACAGAGGAT6820.20584702125476134No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6750.2037342219163694No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC6560.1979994808550197No Hit
CTCTATCTGCTCCCTTTCTCTTCTTCTCACATCCCTTAATTGTGACCTTT6440.19437753913206202No Hit
GGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCA6370.19226473979367004No Hit
GTGCAAAGTTGTGAGGTGTTTTGACGGTCACTGCTAAACCAGGTCTTCCA6180.1865299987323204No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6080.183511713963189No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5830.1759660020403605No Hit
GCTTACTTGATTTGCCAACTCTCTTGTAGGTGCAAGAACCAGTACCTGAG5720.17264588879431597No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA5550.1675148046867926No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5530.1669111477329663No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA5460.16479834839457433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.1644965199176612Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC5330.16087457819470352No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT5080.153328866271875No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC4970.15000875302583047No Hit
GGTCCACGACGAGGGCGTTGCCGGGCACGGTGCCCTCAGTGTCCCCGTGC4880.14729229673361222No Hit
GAGTACATGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCC4830.1457831543490465No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA4800.1448776689183071No Hit
CTTTGGGAGGCCAAGGCAGGAGAATCACTTGAGCTCAGGAATTCGAGACC4770.14397218348756768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.1433685265337414No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4720.14246304110300198No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC4700.1418593841491757No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC4670.14095389871843628No Hit
GAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCC4660.14065207024152315No Hit
AAGCTAACACTGCTCACTTTCTTTTTAACAGGCAAAATATAAATATATGC4620.13944475633387057No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC4560.13763378547239175No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA4540.13703012851856547No Hit
ATGGTGGTCTTCCTCTATCCCTTCCTCAAATGGTGGAGAGACCCCTGCCG4520.1364264715647392No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC4490.13552098613399977No Hit
TTCACATCACAGTGCGGTCATTTGGTTTTTTCCTCTACATTGTGAAAGTG4490.13552098613399977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4440.13401184374943406No Hit
GTGGAGTTGTGCTCATATCTGGAACAGGCTCCAACTGCAGGCTCATCAAC4320.13038990202647638No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC4240.12797527421117127No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC4200.12676796030351872No Hit
GCACTGGGACTCGGATTTCTCTATCTGATAGAGGTATTTTCTGTGGCCCT4200.12676796030351872No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA4170.1258624748727793No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC4080.12314601858056104No Hit
TGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGA4070.1228441901036479No Hit
TAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTC3980.12012773381142965No Hit
ATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAATT3920.1183167629499508No Hit
AAGGATTACATTTCATTGCTTCCCTGTTGAAGTGAAGATATAAATGTTCC3890.11741127751921138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3880.11710944904229825No Hit
CTTCTGTCTTCAGGGAGAGTACAGCTTGTTGTGGGGGATGGAAGAATGGG3880.11710944904229825No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC3850.11620396361155882No Hit
CTTCTTTTCTCTGCTGGGGCTGGGCCGTCCTGCGCACTCCTCCTGTACTC3840.11590213513464567No Hit
CTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCG3770.11378933579625371No Hit
GTACATGGGAGTGCTAAAAAGATGCCTGACAGCAATGTTGATGGGCGAAT3770.11378933579625371No Hit
GAACATTACGCCGCCATGGCCTTGATGAAGCGTTCGCCGAAGATGACGGC3750.11318567884242743No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT3740.11288385036551428No Hit
GCAGTGGTTCAATCTCAGCTCACTACAGCCTCAACCTCCTGGGTTCAAGC3560.10745093778107777No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG3560.10745093778107777No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3540.10684728082725149No Hit
GCACTGAGGAGCAGCAGGAGGAGGTGAGGAAGCGCTGTGAGAGTGCAGAG3530.10654545235033834No Hit
GTCATAGCTCACTGCAGCCTCAAAGTCCTGAGTTCAAGCAATCCTCTTGC3490.10533813844268579No Hit
TCCCGTAGACAGCGGCCCCGAAACGACACTTCGAGTCCTGGAGCGGGAGG3430.10352716758120695No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG3430.10352716758120695No Hit
CACAAGGAGATTGGAAAGAAGCAGCTTCAGTATTTCAGTTGGAAACTAAA3410.10292351062738067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)