FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654122
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8089812.367417698839054No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA205093.135347840311135No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG99551.5218873543467426No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA72951.1152353842249612No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG54120.8273685948492789No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43270.6614973965101311No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG42440.6488086320288876No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC42420.6485028786678938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40180.6142585022365858No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC28780.439979086470108No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA24560.3754651273004118No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC24000.36690403319258486No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA23910.3655281430681127No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG22430.34290239435456993No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA21360.32654458954140053No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC20210.30896377128425584No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17420.26631117742561783No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15950.24383830539257204No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15530.23741748481170177No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14140.21616762622263128No Hit
GTTCCTCCTTGGGCACGAACTGTACCAGGCCGCTCACCACAGTCCGTGGT14040.21463885941766214No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC14030.21448598273716524No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA14010.2141802293761714No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT13990.2138744760151776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.20852379219778575Clontech SMART CDS Primer II A (100% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC13330.20378461510238152No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13300.20332598506089078No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT12940.197822424563002No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC12430.19002571385765957No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12180.18620379684523683No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11890.18177037311082642No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA11880.1816174964303295No Hit
TGCCCTCCTCCACCATGCTGCGGTCCAGCGCGTCCTTATAGGAGATCTTG11840.18100598970834186No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11670.1784070861398944No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10430.1594503777582775No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10420.1592975010777806No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG10050.15364106389939491No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC10030.15333531053840108No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG9900.15134791369194125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9830.1502777769284629No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9600.14676161327703396No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9400.14370407966709575No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT9310.14232818954262355No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC9120.13942353261318224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.13835339584970388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8930.13651887568374096No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8830.13499010887877183No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8810.13468435551777805No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT8610.1316268219078398No Hit
ATACTATTTTGTGTTTCTCCCGCTGTACTCCAGAGTGACACTGGTACACT8460.12933367170038618No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8390.1282635349369078No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8380.12811065825641088No Hit
CTATCACCAGGAACATTTCCATCACCCAAACTGTTCCGCTGTGCCTGTTT7890.12061970091206227No Hit
CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGC7860.12016107087057155No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC7600.11618627717765188No Hit
GGGCAGAAGAATGGTGATATTTACTTCCTAGGAACAGAATAAAGAGGTAG7350.11236436016522913No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT7080.10823668979181254No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC7010.10716655302833417No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6690.10227449925243304No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG6610.10105148580845774No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC6560.10028710240597319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)