FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974321
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT940819.656057911099115No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA237422.436773917425571No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG122711.2594411903263916No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA90110.9248492026755043No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG69180.7100329357573121No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA60440.6203294396815834No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC54950.5639825067919094No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52520.5390420610866439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50700.5203623857024533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.4176241710894048No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA32230.3307944712266286No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT31160.31981246426998905No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA26530.27229219117724035No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC25400.26069437074639673No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC24310.24950709263168916No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC24080.24714647431390682No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA22690.2328801288281788No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC22470.23062214608943046No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC21240.2179979698682467No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG19150.1965471338501377No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18660.19151799047747098No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18640.19131271931940294No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16420.16852762077385175No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT16000.16421692645442312No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC15850.1626773927689129No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT14640.15025848770579717No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14150.14522934433313045No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT13930.14297136159438215No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT13540.13896857401205556No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.13886593843302156Clontech SMART CDS Primer II A (100% over 21bp)
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13480.1383527605378515No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA13400.13753167590557938No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT13260.13609477779910317No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12950.1329130748490487No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC12850.13188671905870858No Hit
CATTACACTGTCAGGGAAGAAGCGCAGAAAACTCCTCCAGCAGATCCGGC12550.12880765168768812No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12070.12388114389405545No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA12060.12377850831502143No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11940.12254688136661326No Hit
CTCAACCTGAGCTGCACCCGGACACTTTAAAGGAGGCATGTGTCCAGAGG11700.12008362746979691No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11630.11936517841655882No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA11300.11597820430843633No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11120.11413076388582406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10950.11238595904224584No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC10830.11115433209383765No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT10630.10910162051315736No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA10420.10694627335344306No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10400.10674100219537504No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC10340.10612518872117095No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT10260.10530410408889884No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG10190.10458565503566072No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT10020.1028408501920825No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAT9990.10253294345498046No Hit
AACAAATACTGTCACTGTCACAGGTATTTTCTAGCTCACCTGCTGGATCC9970.1023276722969124No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT9880.10140395208560629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)