FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445430
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC408999.181914105471117No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA130432.9281817569539546No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG55001.2347619154524843No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA42490.9539097052286555No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG29230.6562198325213838No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC27540.6182789663920256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.5224165413196237No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20970.4707810430370653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.4422692678984352No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC18830.4227375794176414No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA16170.36302000314303035No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15120.3394472756662102No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC13180.29589385537570434No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA12830.2880362795500977No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11680.26221853040881843No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11450.2570549805805626No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC11300.253687448083874No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT10680.2397683137642278No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA10450.23460476393597196No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC9860.2213591361156635No Hit
AAACCAGACTGTGATGACTGGGAGAGCGGGCTGAATGCAATGGAGTGTGC9840.2209101317827717No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA9640.2164200884538536No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT9610.21574658195451588No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9350.20990952562692228No Hit
GGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTC9260.20788900612890912No Hit
GTCACATTGCCCAAGTCTCCAACATGCCTCTCTTCATCCTTTGGCCCACC9000.2020519498013156No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT8780.19711290213950566No Hit
GTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTGCCTCCGAGGGA8750.19643939564016794No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8700.1953168848079384No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC8180.18364277215275127No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC7990.17937723099027905No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT7700.17286666816334778No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC7550.16949913566665917No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7550.16949913566665917No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7470.16770311833509194No Hit
GCTTCTCCCCAATGCAGGCGATTACTCCGAGTCCCTCTGCCAGAGCATGG7290.16366207933906562No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC7180.16119255550816064No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7060.15849852951080978No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7030.15782502301147208No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.1553554991805671Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6900.15490649484767527No Hit
GTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC6730.15108995801809486No Hit
CCTCATAATCCTTCTCAAGGGCAGCCATGTCCTCACGGGCCTCTGAAAAC6650.14929394068652763No Hit
GACTTTATCAAAAACATGATTACAGGGACATCTCAGGCTGACTGTGCTGT6610.148395932020744No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA6440.1445793951911636No Hit
GTGTACCTGGGATGCGCACGACCTCCAACTGCTGGGGCCCATAGTGCAGA5890.13223177603663877No Hit
CAACAGAGAAGCACCGTGGCGCGGCGAGGGAATGCAGACGGCACCCAGCG5870.13178277170374694No Hit
CTCTCATGGTGTCCAAATCCACTAAATAATGGCATTTACTGATATCAATA5740.12886424353995016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.12886424353995016No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT5650.126843724041937No Hit
GTCGTAGAGCGCGTGTGAGACAGACAGGAGCGAGAAGGAGGAGTCCACGG5570.12504770671036977No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC5550.12459870237747794No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT5500.1234761915452484No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT5370.12055766338145163No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG5310.11921065038277619No Hit
GAGTACATGGGCATCATTAGATGGCAGGTATTTTTGGAACCGTCAGGACT5290.11876164604988437No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC5280.11853714388343847No Hit
GAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAA5260.11808813955054666No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5190.11651662438542532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5110.11472060705385806No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5070.11382259838807446No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC5030.11292458972229083No Hit
CTGTGTGGGCGTAGTGGCGGGCGGCAGTGCTATACTCCACATGAGCCGCA5030.11292458972229083No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGC4830.10843454639337269No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC4790.10753653772758906No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC4790.10753653772758906No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA4700.1055160182295759No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG4670.1048425117302382No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC4620.10372000089800867No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG4590.10304649439867096No Hit
GGCCTTGCCGAAATGCCGGTACACCTCTCGGCCCTTGCGAGGACCGGAGA4570.10259749006577915No Hit
GGATGAAGACTACATCCAGGACAAATTTAATCTTACTGGACTCAATGAGC4540.1019239835664414No Hit
GAACTTTAGCTGCAGGATGTTCTACCTCCAGTAACTTCAGGATGGTTGCA4520.10147497923354959No Hit
GTTTATGAAATGCATATGGCTTGTCAGAGCTCATTCGACAGTTAAAGCCA4460.10012796623487417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)