FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563207
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT384176.821115504601327No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA104371.8531374787600297No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46490.8254513882107289No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA39250.6969018495863866No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA38480.6832301445116982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37480.6654746833757392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.5878833182115989No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26190.4650155271507634No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC22610.4014509762840306No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC21930.3893772627115785No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21320.37854643141864364No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20360.3615011887281231No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGG16310.28959157112748957No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC15790.2803587313367909No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15540.2759198660528012No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15300.2716585553801711No Hit
CTGCATGGCCTTCATGACATGAAGGTTGGGCACATTCTTGTCTGCCAGCT14270.2533704304101334No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14090.2501744474056608No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA13000.2308209947674656No Hit
GAAGATGGCTTTGCGGTAAGACACTCCGTCAACCTGCTCGTAGTAGTCTA12410.22034527269724985No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12360.21945749964045191No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12350.21927994502909232No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12110.21501863435646218No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12090.214663525133743No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11850.21040221446111287No Hit
GTCCCAGGCGGCGTGGGCCTCGGCCGTGAAGTCGGCGGGGAAGCGCGCGG11640.2066735676225615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.20223470233857177Clontech SMART CDS Primer II A (100% over 21bp)
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11170.19832850088866086No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10380.18430168659125332No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT10210.18128325819814028No Hit
AGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC9980.17719950213686975No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9800.17400351913239714No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA9580.1700973176824862No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA8650.1535847388260444No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC8500.1509214196556506No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8260.14666010898302045No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8130.14435189903534582No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC8110.14399678981262662No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC8040.1427539075331095No Hit
GAACTTCATTGACCCCCTCCAGAACCTGTGCGAGAAAGACCTGAAGGAGA8030.14257635292174992No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8030.14257635292174992No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7970.14151102525359238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7930.14080080680815402No Hit
CACAGACCTCGTGCGAGGGACCCTCTGCCCGGCCCTTAAGGCCCTGTTCG7830.13902526069455812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.13849259686047935No Hit
GGCAAGAGGTCCCCTCTGAAGCACATGGGACCAGGACACCCAGGATGGCA7800.13849259686047935No Hit
GAACTCCTCGTACCAGGACATGGCCGCTGGGTGTCGGGGCACGTCGCGGG7660.1360068323014451No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC7440.13210063085153415No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT7220.1281944294016232No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7210.12801687479026364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7110.12624132867666774No Hit
GACGGGGGAGGGGCCGCAGCCTGTCCTGGGGGTCCCGGAGGCGGCGCTCA7090.12588621945394857No Hit
GTAGCTAGCCGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCAC7070.12553111023122937No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC7020.12464333717443143No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA6920.12286779106083554No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG6910.12269023644947595No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6750.11984936266772252No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG6650.11807381655412665No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT6620.11754115272004786No Hit
CTTTTAATGGGAACCAGAGGTATAGTTACAATTACATAGTCCGACACAAA6450.11452272432693486No Hit
AGGTGAAGATGAAGAACAGAACCAGGAGCACAGTGAAGATGATGAAGACG6360.11292473282469855No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG6360.11292473282469855No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT6270.11132674132246226No Hit
GTTCAACCAGGTCCTGGATCAGTTTGGGGAGAAGTTTCAAGCAGATGGGA6240.11079407748838348No Hit
AACTTGCCGATAGTCTTCCTTGTGCAGAAGGAGAGTTTATCTTCCTTCGG6220.11043896826566431No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC5960.10582254837031499No Hit
GGACGGCGACATCCAGCATATCCCCGGCATGGTGAAACTGATCACGTTCT5950.1056449937589554No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT5940.10546743914759582No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5880.10440211147943827No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG5810.10315922919992117No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA5730.10173879230904447No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC5710.10138368308632527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)