FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565299
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5978210.575288475656246No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA133062.353798609231575No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG68861.2181164304200078No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48210.8528230193225178No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG44350.7845405705653113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36850.6518674188349882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.6002133384279824No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30050.5315770945994952No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC29810.5273315537441248No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA29380.5197249597115863No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA24410.4318068844982921No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA20660.3654703086331304No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17480.30921689229947336No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC17290.3058558391223052No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16140.2855126225236556No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC15700.2777291309554767No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT13670.24181893122046916No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA13370.23651200515125625No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC13120.23208956676024545No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12440.22006053433669617No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC11500.20343216598649566No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT11430.20219388323701265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.19387969906191235Clontech SMART CDS Primer II A (100% over 21bp)
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10750.19016485081346332No Hit
GACTCGGAGTAATCGCCTGCATTGGGGAGAAGCTAGATGAAAGGGAAGCT10700.18928036313526117No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10590.1873344902432164No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10540.18645000256501426No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9940.1758361504265884No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT9910.1753054578196671No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9760.17265199478506066No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9570.16929094160789246No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA9360.1655760933594434No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA9190.1625688352535561No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8800.15566983136357926No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT8730.15443154861409628No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8590.15195498311513023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7860.13904146301337875No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG7820.13833387287081705No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC7780.1376262827282553No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7680.135857307371851No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7620.1347959221580084No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC7550.1335576394085254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.1324962541946828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7330.12966589362443592No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7240.12807381580367205No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7090.12542035276906557No Hit
GTGGCTGAGTTGGGCACCAAGGACCTGAAGGAGAAGGTGGAGGAGGAGGC6910.12223619712753782No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT6870.12152860698497608No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGAT6680.11816755380780791No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC6670.11799065627216747No Hit
CTTCCGCAGGGAGAGCCTGGCTGAGGAGCACGAGGGGCTCGTGGGTGAGG6620.1171061685939653No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA6610.11692927105832489No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC6500.11498339816628014No Hit
GTACATGGGGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTAAGGCAGGA6480.11462960309499928No Hit
CATAAAGTGTATCTGGAGGGCCAATAATAAGGACTTCCCATCGGTAGAGA6470.11445270555935885No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6430.11374511541679712No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA6330.11197614006039282No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC6320.11179924252475237No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACT6220.11003026716834809No Hit
CGTGGTTTTTCTGCCTCATCTCCAGAGCTATCTTCCTGAAAGGCTGCATA6030.10666921399117989No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC5920.10472334109913514No Hit
CAGATGATTCAGGCCAAGCTCTTAAAGGCAGATCTTCACGGGGCTATTAT5860.10366195588529256No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT5850.10348505834965212No Hit
GGCAGAACCCAGGGCATGAAGATCCAAAAGGGCCTGGTTCAGCTTTTTCT5750.10171608299324783No Hit
CAATGATAATGAGCCATTCAAGCCTTGGAAGGGATTAAAAACCAACTTTC5700.10083159531504567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)