FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559268
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA426507.626039751961493No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA123342.205382750309333No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG47970.8577283162991625No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA46650.8341260361758585No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA37660.673380204123962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34240.6122288419863107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.5385611191772102No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG28330.5065549968887904No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC27730.49582668774183397No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC23860.42662909374396535No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23700.42376821130477693No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20790.37173591194203853No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC20600.36833861404550233No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG14790.2644528204724747No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14150.25300929071572126No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13740.24567827946530107No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT13490.24120815065406925No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13300.23781085275753305No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA13130.23477116516589544No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT12770.22833417967772157No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12710.22726134876302598No Hit
TTTTTGTAGAGACGGCATCAAAATATGTTGCTCAGGCTCACTTTGTTTTC12380.22136077873219995No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11020.19704327799909882No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC10810.1932883697976641No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG10430.18649377400459172No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.18631496885214244Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10370.18542094308989607No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA10230.18291767095560627No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9980.17844754214437442No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9800.17522904940028752No Hit
ATGTCATCAATTTTTTCTTGAGACTGTCTGCGTTTCTGGATTGCCTTATA9400.1680768433023166No Hit
GAGGAGAGAAGAAGTTGTAAATGTCGTTCTCGGTCGCTTTGTACGGCAGG9050.16181866296659206No Hit
GTAAAGTGCCTTGAGCTCCCCCAGACAGAGAAGAAGCAGAGGTGTCACTG8950.16003061144209932No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA8650.15466645686862113No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT8620.15413004141127332No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8570.15323601564902695No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC8400.1501963280573893No Hit
GTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATT8290.14822947138044731No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG8290.14822947138044731No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8270.14787186107554876No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT8210.1467990301608531No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8150.14572619924615748No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC7990.14286531680696912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.13660713647124456No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC7610.13607072101389672No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT7340.13124298189776637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7290.13034895613552No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC7150.12784568400123017No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC7140.1276668788487809No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7070.126415242781636No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA6970.12462719125714326No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT6910.12355436034244764No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6680.11944184183611435No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6630.11854781607386798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6550.1171173748542738No Hit
CCATACACTCTTCTAAGGTTTCAGAATCATTACTAATCATAGAAGGACTC6500.11622334909202744No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT6300.11264724604304198No Hit
GTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT6280.11228963573814343No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6100.10907114299405651No Hit
CACCTCTGCTGAGCCTTCACAGAGGGGGTCCTTGACCTTTGCTCTCAGGA5800.10370698842057834No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC5750.10281296265833195No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5720.10227654720098416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)