FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378927
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC4834912.759449709310763No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA129293.4120028395970725No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59291.564681323843379No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA44711.1799106424192523No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC31550.8326141974575578No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31180.8228497837314311No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG23440.6185888047038084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.5008880338429301No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC17580.46394160352785624No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA16560.43702349001258817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15240.40218828428694714No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC14340.37843700765582816No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13330.35178279721423916No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC12710.335420806646135No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12130.32011442837274723No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12070.3185310099306727No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC10450.2757787119946586No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10130.26733381363692743No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10050.26522258904749463No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9870.2604723337212708No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC9700.25598598146872614No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT9580.252819144584577No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9170.2419991185637339No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT8980.23698496016383103No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8900.23487373557439825No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8890.23460983250071912No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8610.22722054643770437No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7760.20478878517498095No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7570.19977462677507807No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA7410.1955521775962125No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC7400.19528827452253336No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7400.19528827452253336No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6930.1828848300596157No Hit
GTGATCACCAGCAGCTTCTCTGGGTTGACCGGGTCTCGGCCCCGGGGCCG6790.17919018702810832No Hit
GTACTGCAATTTCGCCGGGCTCCTGGCCGAGACAGTGGGGAAGTCGTTAC6100.16098087494425048No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC5890.1554389103969894No Hit
GTACAAGCTGCTGGAGCCAGTTCTGCTTCTCGGCAAGGAGCGATTTGCTG5810.1533276858075566No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC5690.1501608489234074No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC5680.14989694584972832No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.14804962433397462Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC5490.14488278744982544No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA5480.1446188843761463No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC5350.14118814441831806No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC5210.13749350138681066No Hit
GTCAGCGCTTCATTATCCATTTTTGTAAGTAGTCCAGTGCCTCCAAACGA5060.13353495528162415No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT5030.13274324606058688No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC4960.13089592454483318No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4860.1282568938080422No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC4860.1282568938080422No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG4810.1269373784396467No Hit
TTCTTAGGTTGTGAGGGAGCTGGGAGTCTCTCATCCGCACTTAGTAAAGC4760.1256178630712512No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4720.12456225077653481No Hit
CCTCTTGGCACCTCAGAATCACAGTGTTACTGATCAGGGATGTGAGGCTG4700.1240344446291766No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC4570.1206037046713483No Hit
TGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGA4480.11822857700823641No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT4460.11770077086087823No Hit
GATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCT4430.11690906163984091No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG4370.11532564319776634No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG4290.11321441860833352No Hit
CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT4190.11057538787154254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.11004758172418434No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4150.10951977557682613No Hit
GATACTTGGTGGTGTGAACAGACACTCATGGCTCCCGTGTGAGGAGCCCG4100.10820026020843065No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT4090.10793635713475155No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC4050.10688074484003515No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG4040.10661684176635605No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG4030.10635293869267697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3990.10529732639796055No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT3980.10503342332428146No Hit
ATTCAAAAGTAAGACGAATAGTCAAAAGGGAGCCTCTTCTACCTGGATGA3950.10424171410324418No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG3850.10160268336645317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)