FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634166
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT6816710.74907831703371No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA170232.684312940145009No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG81501.2851524679658008No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA61030.9623663204902186No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG44190.6968207062504139No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC42520.6704869072135686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.5979506942977075No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG35080.5531674671931325No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA31830.5019190558938826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.4771621310508605No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC27020.4260714071709931No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA21570.34013176360763586No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT20350.32089389844299443No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT18610.29345628747047303No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18440.2907756013409738No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC18360.2895141019859154No Hit
GAATCATGTCTGGTCGCGGCAAAGGCGGAAAAGGCTTGGGGAAGGGTGGT17000.2680686129499216No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16380.2582919929482186No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA16370.2581343055288363No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT15960.2516691213341617No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC14510.22880444552372722No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14090.22218157390967033No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT13730.2165048268119073No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13520.21319339100487883No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12820.20215527164811736No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC12640.19931689809923586No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC12000.1892249032587682No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11940.18827877874247437No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11630.18339046874162285No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11470.18086747003150597No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC11410.1799213455152121No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.1794482832570652Clontech SMART CDS Primer II A (100% over 21bp)
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT11140.17566378519188983No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11060.17440228583683137No Hit
TGCTAAGGGTGGGAAAAGGTTTGGCTAGATATTGAAAGATATTGACTGGC11060.17440228583683137No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA10570.16667560228709832No Hit
AAACTGGGGAGAAGCATGCCTCCTGTCCCCAGGGAGAAGGCAGGAGCGAA10390.16383722873821682No Hit
ATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGG10270.16194497970562913No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10130.15973735583427684No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10000.15768741938230682No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC9390.1480684867999861No Hit
TCTGAATCCCACTCAAGTCACCATTTGCCCATAAATCAGTGAAACCCGAA9290.14649161260616306No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9160.14444167615419307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8520.13434968131372543No Hit
GCCAAAGGTGGGCACAACGTTGACAAAGCGCCGGTTGTACTGCATCCGCC8310.131038245506697No Hit
ATCTCGTCGCTTACTGGGAGTCTGCTATATTGTTGTTAAGGTCTCTTTGT8230.12977674615163853No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8190.1291459964741093No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8030.12662299776399238No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC7970.12567687324769855No Hit
GGTTCTTCAAAGACATTTCCAACAAGGAAAGTTGCTAAAGAAGGTGGACC7820.12331156195696394No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7720.12173468776314088No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7700.12141931292437627No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC7660.12078856324684703No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC7410.11684637776228936No Hit
TTCTTGTAGCTGCTCTCCAATTTCCTATTACCCAAGGTAATTAGCCTCTG7390.11653100292352477No Hit
TCATCATCTTCTCCAGTTCCTAATCCAAGTGATCCAATTTTATCACTGAT7320.11542719098784861No Hit
GAGAGGCATCCTCCAGACACGGTCCCCTGTTTCAATGCTGGCCTCGAAGA7250.11432337905217246No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT7220.11385031679402553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7050.11116963066452631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7030.11085425582576171No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7020.1106965684063794No Hit
GTTCTCGTTTGGAAAACCTTGGCATTCCTGAAGAAGAGTTATTGAGACAG6940.10943506905132096No Hit
CTCCTCCAGGGCCCGCAGCAACTGCTTCATGGTGATCGTGCGCTCCACCA6930.10927738163193865No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT6930.10927738163193865No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6850.10801588227688018No Hit
TCCACGCCCTGCGGATCGGCGAGCCGATCCAGCAGCGCGTCGCCATCCTG6840.10785819485749788No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG6820.10754282001873326No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT6520.10281219743726407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.10249682259849945No Hit
TACCATATGACCAGAGCCCAGGCCCAAAGCATCTTGCTTGGTTGCTACAT6480.10218144775973484No Hit
GTACATGGGGCTGCCAACTTCAGACGAACAGAAGAAACAGGAGACTCTGA6410.10107763582405868No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC6390.10076226098529406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)