FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700305
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG373745.3368175295050015No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA239703.4227943538886625No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC95561.3645483039532775No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA94431.3484124774205524No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC63520.9070333640342423No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT57850.8260686415204804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51380.7336803250012495No Hit
GGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCA51290.7323951706756342No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG50580.7222567309957805No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA49370.7049785450625085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48440.6916986170311507No Hit
CCGCGAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCA48420.6914130271810139No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT47730.6815601773512969No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC44580.6365797759547626No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG40060.5720364698238625No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC27460.39211486423772496No Hit
CATGAAGACAGCCAGCTTCAAGGAGTCAACCAGATCTTCTACCAGAAAGA27400.3912580946873148No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC26370.3765502174052734No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC25300.3612711604229586No Hit
ATGAAACTATGCACATATTGAAAGTGAGTTGAAACAAATGAGGGTTGGGT24290.34684887299105394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23760.33928074196243063Clontech SMART CDS Primer II A (100% over 21bp)
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22660.3235733002049107No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG22330.3188610676776547No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC22170.31657634887656094No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT21180.302439651294793No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA19840.2833051313356323No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC18790.2683116642034542No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCACAAAGCACCTGGATG18080.2581732245236004No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG17340.24760640006854157No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA15220.2173338759540486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14820.21162207895131407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14810.21147928402624572No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13040.18620458228914546No Hit
GTATCAACGCAGAGTACATGGGCATGCACCTGTAGTTCTGGCTACTCCAG12640.1804927852864109No Hit
GTACATGGGCATAGCGCTTGCGGCCGCCGCATAGTCAGGAACATCGTATG12280.17535216798394987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11870.169497576056147No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11720.16735565218012152No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT11640.1662132927795746No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC11470.16378577905341243No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC10910.15578926324958411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10490.14979187639671288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.14850672207109758No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT9400.13422722956426128No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8610.1229464304838606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG8510.12151848123317698No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA8470.1209473015329035No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC8180.11680624870592099No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT7990.11409314512962208No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG7740.11052327200291301No Hit
GCGCGAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCA7720.1102376821527763No Hit
TCGCGAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCA7610.1086669379770243No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG7510.10723898872634066No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC7500.1070961938012723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)