FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306368
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG863956.613373873211836No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA280432.1466386194395457No Hit
TCTCTGAGCAATGGACATAGCTCTCATATGAACCTTTTCAGCTAAAACCT178871.3692160248873213No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC158861.2160432588673329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121390.9292174946110131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101060.7735951891044484No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG100200.767012051734274No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA98050.7505542083088379No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT96900.7417511757789536No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA88260.6756136096413875No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84550.6472142612188908No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC78700.6024336174799138No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC69950.535454022143837No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC67540.5170059278855575No Hit
GTGTAAATTTTAGAATGAGGCAGACAAAAGTTGGAAGGCCGGTTAATTTT62940.48179379776602No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC57490.44007507838526355No Hit
GTAGCAATCTTCTTACCATCGACAATGATGTGCTTGTCATCGTGGGAAAC55600.425607485792671No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG49930.3822047080148932No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46780.3580920536939055No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC40420.30940745639819717No Hit
ACGCAGAGTACATGGGAGATGCAAATAACTCCTTCACCAGGCAGTTAGTG40350.3088716196355085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37240.2850651577503429Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG36230.2773337987458358No Hit
GTCAAGGCCCATGGCAAGAAGGTGCTGACTTCCTTTGGAGATGCTATTAA35920.27496080736821477No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT35050.26830112188908484No Hit
GGGCAGCTGGAAGATTTGGTCTTGAACTTGGGGGGTGGGTAAGTGATGAT33700.2579671271800902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33130.2536038849696257No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG32570.2493171908681168No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC29270.2240563149127964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27550.21089004017244756No Hit
ATAAAATTCAAACTGCTTAGTCTGCTTTCAAATTCTTCCAAAAGTGAACC27130.2076750195963159No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25380.19427910052910052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23600.1806535371350186No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC22930.17552481383499902No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA22900.17529516950813248No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC22760.17422349598275524No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA22180.16978370566333528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21420.16396604938271603No Hit
GCATCCACGTCCGAGGCCACGAAGAGAAGGACCCTCTGGCTCTGGACCAC20240.15493337252596512No Hit
GTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCC20100.15386169900058788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19320.1478909465020576No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA18860.14436973349010387No Hit
CTGATGGGCTGTCCCATCAAATCCTGGCCATTGAGTCCCTCCATAGCAGC18640.1426856750930825No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA18600.14237948265726044No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC17930.13725075935724085No Hit
GAGGGAGGTAGGTGGTAGTTTGTGTTTAATATTTTTAGTTGGGTGATGAG17640.13503086419753085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG17510.13403573878110916No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG16580.12691676464824614No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16470.12607473544973544No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15990.12240042621987067No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15410.11796063590045072No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14990.11474561532431903No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA14490.1109182098765432No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG14150.10831557417205565No Hit
GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTC13870.10617222712130119No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT13770.10540674603174603No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13710.10494745737801295No Hit
GTTCTTGAACAGACAGAAGGATGTAAAGGATGGAAAATACAGCCAGGTCC13690.10479436116010189No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA13130.10050766705859299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)