FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073712
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT545025.076035286929828No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA209111.9475427302665893No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC157861.4702266529572177No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCT109901.0235519394399988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97420.9073196536873946No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT94330.878540986782303No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA86800.8084104489844577No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84590.787827648382434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83930.7816807486551329No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC80850.7529952165943941No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA77470.7215156392030637No Hit
TCTCTGAGCTGGTCAGCACAGCCTGCGGTTTCCGGCTGCACCGCGGCATG75410.7023298612663358No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG64430.600067802166689No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC54580.5083299804789366No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC53400.497340068845277No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC52290.4870021011220886No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG48160.44853741040427975No Hit
GGTCTACACACAGTGACTTTATTACTCTATGGATGCTGGTGAACTGCCCT45250.42143517069754277No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC40470.37691671509678576No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG37110.3456234073941616No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT36500.3399421818886256No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.32215342661719343Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG31880.29691388379751743No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30060.27996334212526264No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC30050.2798702072809096No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC29450.27428211661972673No Hit
GTCTTTTGGGACACGAGGTCCTCAAAGGTGGTGGAAGATGAACAAAACCA29230.27223315004395965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27600.2570521704144128No Hit
CCTTGGAGCCGTGCGCGCGCAACTGCGCGGACCCCGGGTGCAGGTCGAAG26010.24224373016227813No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25470.23721444856721355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG23950.22305795222555022No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA21550.2007055895808187No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC21030.19586257767446022No Hit
TCTCCAATCCATTGAACTTCGGTGCAGACATCGTTGTCCACTCCGCTACA20050.18673536292786147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19750.18394131759727003No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA19640.18291683430938652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.1693191470338415No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA17660.16447613512748296No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16550.15413816740429465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16250.15134412207370318No Hit
GTCCTGGGTTAGTTCTGATTCCTCACAAGCCCTAAGACAAGATTTTGAAA15960.1486432115874648No Hit
TTGCCATGGTGGCCCCGCAGACCCAGGCCCCTGCTCAGCAGCAGCCTAAG15810.14724618892216906No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15760.14678051470040382No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG15240.14193750279404535No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG14250.13271715320309357No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT14030.1306681866273265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG13840.1288986245846186No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13830.12880548974026554No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA13820.12871235489591248No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT13550.1261977140983802No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13270.12358993845649487No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT11940.11120300415753946No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA11870.11055106024706811No Hit
CTTCCACATAGGGGAACTGTGGCTCTGGGGGCAGCTGGCTTAGGGAAAGG11360.10580118318506265No Hit
GTTCTCACGTTGAGGTCTGTGGAAGAGATGTCCATTGGAGAAATGGCTGG11230.10459043020847303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)