FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041889
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT478174.5894524272739226No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA182781.7543135593139No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC145911.4004370907073593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96530.9264902499210569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83680.8031565742607898No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT79440.7624612602686083No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT75960.729060389350497No Hit
ATATAATCGACCGTTTCACCAACACTTCTCAACTCATCAGCCACTTTGTC75300.7227257414177518No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA75120.7209981101633668No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC69000.6622586475142745No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA65160.6254025140873932No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC53220.510802974213184No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG52580.5046602853087038No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52060.49966935057381356No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC47010.45119969593689924No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT46920.4503358803097067No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC46150.4429454577215039No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC37480.3597312189686233No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC35410.3398634595431951No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.30895805599252896Clontech SMART CDS Primer II A (100% over 21bp)
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT32150.30857413793599897No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31320.30060783826300114No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG30730.29494504692918344No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA30320.29100988684975077No Hit
TCCCAAATGTTATTTCTTGGCATAGGTGATCTTCATAGCATGGGACGGTG27670.2655753156046374No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG26990.2590487086436271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26460.2539617943946044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25110.2410045599867164No Hit
TGATGCATCAGCAGCCTCCTTCTCAGCAATACAATATGCCACAGGGAGGC24190.23217444468652612No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA23250.22315237035807076No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG23220.22286443181567328No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA22660.2174895790242531No Hit
CCCAGGTGGTGTCGGGTTTGACATCAACTGTGGTGTCCGCTTGCTAAGAA22200.2130745213741579No Hit
GCCCACTCTGGAGTTCTCCCACCCTCACAGGTTCTGGCCTGCCATTGACG19100.18332087199308178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19060.1829369539365518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18830.1807294251115042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16640.15970991151648592No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16550.15884609588929338No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA16330.1567345465783783No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC16080.15433505872506573No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14520.13936225452039516No Hit
GTGGTGCCCAAGGTGAACCCAACTTTCAGCCAGTCAGGTTTGGCATTCGG14370.13792256180840762No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT14360.1378265822942751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG14290.1371547256953476No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC14240.13667482812468507No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13080.12554120448531467No Hit
AGAGACTTCCTCCACTGCCTCTGCCTGTCTCTTATACACATCTCCGAGCC12450.11949449509496693No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12260.11767088432644937No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT11830.1135437652187517No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11800.11325582667635418No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT11580.11114427736543912No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11330.10874478951212654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11250.1079769533990665No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10900.10461767040442888No Hit
TATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACA10760.10327395720657383No Hit
CGAGTAAACAAGCCAGAAAGTGGAGTTCTGTCCGCTGCGAGCCTGGAGAT10750.10317797769244133No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10510.10087446935326125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)