FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141354
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1009528.84493329852088No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA382303.3495304699506026No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA136621.1969993533995587No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC124831.0937009902273966No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG112130.9824296405847791No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC94630.8291029776914087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90020.7887123539234979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79980.7007466570406727No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT72070.6314430054128694No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT66950.5865840046120661No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA64990.5694114183680086No Hit
ACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGC64270.5631031213803955No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC59490.5212230386015206No Hit
GGGCCTGCCAGCCAGCACAACAGAGCAGGTTTTTGCAGTAATGATAGATC58780.5150023568498469No Hit
AACCTGTACCACCATGCCTAAAGATTAACCCTTGTAGGCCGGATGCGGTG58060.5086940598622338No Hit
GTACATGGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGT52920.4636598285895524No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC52490.4598923734441725No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52350.4586657601410255No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG47810.4188884430246882No Hit
CAAATACCCTTTATTTCTTTCTCTTGCCTGACTGCCCTAGCCAGAAATTC46730.4094259975432688No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC38730.3393338087920137No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC35190.3083180152695833No Hit
AGGCTGCAGTCAGCTGCTACTACCGCACAGCAGAACTCTCTCTCAAAAAC33190.2907949680817696No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC32250.28255913590349707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.28045637024095943Clontech SMART CDS Primer II A (100% over 21bp)
ACCTGGGACTCCCCTTGGACATGGTAGAGCTGATGCTGGAGGAGAAAGGG29680.2600420202671564No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG28100.2461988129887835No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC27280.23901436364177986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27270.2389267484058408No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA26990.23647352179954687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24770.21702293942107356No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC24660.21605917182574383No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT24360.21343071474757175No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG23460.20554534351305553No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC22780.19958750746919884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG21430.18775945061742458No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA21380.18732137443772923No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA21190.1856566849548869No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC19530.17111255578900147No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC18780.16454141309357131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18700.16384049120605876No Hit
AGAGACTTCCTCCACTGCCTCTGCCTGTCTCTTATACACATCTCCGAGCC16940.14842020968078265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.14710598114169662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG15900.13930822514311947No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA15560.13632930712119115No Hit
ATGCCACTGCTGTAACACCAATGTTCCTCCGCAAATGGACTGAGACGGCT14910.13063431678515167No Hit
GTTCAAAGACCCAGAAGCCGGCAAACCAGGGCTGACCCGCGCCGTCCAGC14620.12809347494291867No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT14470.12677924640383265No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14400.12616593975225915No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA14350.1257278635725638No Hit
CTGCAAGCCAGTGAGCACATACTGGCCAGGTGTGGTCGTGCTCTCCCGTA14290.1252021721569294No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14050.12309940649439174No Hit
GCCCTGCTGGGCCTGAGCCCGACGTTCTTGCTTCAGGGCCAGGTGCAGCT13850.12134710177561037No Hit
ACTTCGCCCCGGACGAGCTGACGGTCAAGACCAAGGATGGCGTGGTGGAG13710.1201204884724634No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC13310.11661587903490066No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13080.11460072860830206No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC12970.11363696101297231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG12370.10838004685662819No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT12250.10732866402535936No Hit
GTATGAAGAGTCTGGTCATCAAACACATTTACCACCAGCCTTCAGAGTAC12240.10724104878942028No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC11660.1021593651049543No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA11550.10119559750962455No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)