FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192643
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA175479.108558317717229No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA72383.7572089305087655No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC70023.634702532664047No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG66973.4763785862969327No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT51392.6676287225593454No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA31821.6517599912792054No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC23371.2131247956063806No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC21681.1253977564718158No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC21061.0932138722922713No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC15350.7968106808967883No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT15040.7807187388070161No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC14130.7334811023499427No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC14130.7334811023499427No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG11520.5979973318521825No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT11320.5876154337297488No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG10750.5580270240808127No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT10740.5575079291746909No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC10020.5201330959339296No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA8770.4552462326687188No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA8400.43603972114221645No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA8360.4339633415177297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.3934739388402382No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT7280.3779010916565876No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC7270.37738199675046585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.37738199675046585No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCT6940.36025186484845023No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA6730.3493508718198948No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCT6580.34156444822806953No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6400.3322207399178792No Hit
GTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGC6390.33170164501175753No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTC5770.29951776083221293No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT5530.2870594830852925No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCTGTCTC5440.2823876289301973No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC5430.28186853402407563No Hit
GATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCG5300.2751203002444937No Hit
TCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC5280.27408211043225034No Hit
ACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAAT5230.2714866359016419No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA5210.27044844608939855No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT4910.25487559890574796No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA4900.2543565039996263No Hit
CGTGGTTTTTCTGCCTCCCGAACCAGAGCTGTCTCTTATACACATCTCCG4900.2543565039996263No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG4860.2522801243751395No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA4790.2486464600322877No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT4640.2408600364404624No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCT4610.23930275172209733No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA4560.23670727719148893No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCT4410.22892085359966363No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA4360.2263253790690552No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT4300.22321080963232506No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC4240.22009624019559498No Hit
GGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGG4150.2154243860404998No Hit
CCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACC4060.21075253188540458No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC3990.2071188675425528No Hit
GCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCA3910.2029661082935793No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA3870.20088972866909258No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT3630.1884314509221721No Hit
GATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTC3580.18583597639156366No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC3560.1847977865793203No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCCTGTC3450.17908774261198177No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCCT3160.16403399033445284No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT3140.1629958005222095No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC3080.15988123108547936No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCT3050.15832394636711428No Hit
CAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGT3030.15728575655487093No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACCTGTCTCT2970.15417118711814082No Hit
GCAATCGCAGGCACTGACGACTCTCACCCTGTCTCTTATACACATCTCCG2920.15157571258753239No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT2870.14898023805692395No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCT2870.14898023805692395No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2870.14898023805692395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGC2860.14846114315080228No Hit
GGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTT2840.14742295333855887No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG2790.14482747880795044No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCT2720.14119381446509865No Hit
AATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTT2690.1396365297467336No Hit
GTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCT2670.13859833993449022No Hit
CAGACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC2670.13859833993449022No Hit
GGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTC2600.1349646755916384No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC2520.13081191634266492No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTG2500.12977372653042157No Hit
GTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTA2430.12614006218756976No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCTG2400.1245827774692047No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCTTATAC2370.12302549275083963No Hit
AGGGTATTTGGTTTCCCGAACCAGAGCTGTCTCTTATACACATCTCCGAG2370.12302549275083963No Hit
ATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTT2340.12146820803247457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2340.12146820803247457No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA2300.11939182840798783No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG2290.11887273350186615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.11471997425289265Clontech SMART CDS Primer II A (100% over 21bp)
GTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGCCC2170.11264359462840592No Hit
ATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA2150.11160540481616253No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTC2140.11108630991004086No Hit
TTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTG2120.11004812009779749No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGC2100.10900993028555411No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT2060.10693355066106736No Hit
GCGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGG2060.10693355066106736No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT2020.10485717103658063No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT2020.10485717103658063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.10433807613045892No Hit
GAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGG2000.10381898122433725No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG1950.10122350669372882No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1950.10122350669372882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1950.10122350669372882No Hit
GTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCACCTGTCTCT1950.10122350669372882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)